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| Other Sizes |
| Targets |
PIPES disodium does not have a specific biological target as a drug. It is a zwitterionic buffer used to maintain pH in biochemical and biological experiments. The disodium salt form provides the buffering capacity of PIPES in aqueous solutions. PIPES buffer is used in various applications including electron microscopy, protein chemistry, and cell biology. It can prevent glutaraldehyde fixation-induced lipid loss and artifacts, making it valuable for sample preparation.
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| ln Vitro |
Dissolve 173 g of 1,4-piperazinediethanesulfonic acid in 1 L of deionized water to create the pH PIPES buffer. Particles of sodium hydroxide are added to the PIPES buffer to bring its pH down to 6.8. As a PIPES buffer, PIPES disodium can be employed to stop lipid loss and fixation-related artifacts [1].
PIPES disodium does not have direct in vitro biological activity as a pharmacological agent. Its value is as a buffer for biochemical and biological research. The compound maintains a stable pH environment for experiments without interfering with the biochemical processes being studied. It is used in buffer solutions for various in vitro applications including protein purification, enzyme assays, and electron microscopy sample preparation. |
| ln Vivo |
PIPES disodium does not have in vivo biological activity as a pharmacological agent. It is not intended for administration to animals or humans as a therapeutic. Its value is as a laboratory reagent for biochemical research. The compound is used in buffer solutions for various in vitro and ex vivo applications.
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| Enzyme Assay |
In vitro biochemical assays using PIPES buffer are performed to maintain pH during experiments. The buffer is prepared at the desired pH (typically around 6.8) by dissolving PIPES acid and adjusting pH with sodium hydroxide. PIPES disodium can be used to prepare the buffer directly. The buffer is used in electron microscopy to prevent lipid loss and artifacts during glutaraldehyde fixation. It is also used in protein chemistry and enzyme assays.
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| Cell Assay |
Cell culture experiments using PIPES buffer are limited as it is primarily used as a buffer in biochemical assays and sample preparation rather than in cell culture media. The buffer may be used in cell lysis buffers or in fixing solutions for electron microscopy. Researchers should consult primary literature for specific applications.
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| Animal Protocol |
In vivo animal experiments are not applicable to PIPES disodium as it is a buffer reagent rather than a therapeutic agent. The compound is not intended for administration to animals. Its value is as a laboratory reagent for biochemical research. Researchers should not attempt in vivo administration of this compound.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not applicable to PIPES disodium as it is a buffer reagent rather than a therapeutic agent. The compound is not intended for administration to animals or humans. Its physicochemical properties include molecular weight 346.33, molecular formula C8H16N2Na2O6S2, and CAS number 76836-02-7. Storage: 4°C, sealed, away from moisture. These properties are relevant for buffer preparation rather than pharmacokinetics.
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| Toxicity/Toxicokinetics |
PIPES disodium is considered a safe buffer for laboratory use with minimal toxicity at typical working concentrations. Good's buffers are designed for minimal interference with biochemical processes. However, as with all chemical reagents, appropriate safety precautions should be followed. The compound is for research use only and is not for human therapeutic use.
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| References | |
| Additional Infomation |
Piperazine disodium salt is an organic salt, specifically the disodium salt of 2,2'-piperazine-1,4-dimethyldiethanesulfonic acid (PIPES), a buffering agent. It possesses buffering properties. It contains 2,2'-piperazine-1,4-dimethyldiethanesulfonic acid.
PIPES disodium has CAS number 76836-02-7, molecular formula C8H16N2Na2O6S2, and molecular weight 346.33. Synonyms: PIPES disodium salt, disodium pipes. It is the disodium salt form of 1,4-Piperazinediethanesulfonic acid (PIPES), a zwitterionic Good's buffer. Purity: ≥99%. Storage: 4°C, sealed, away from moisture. Not for human use; for research purposes only. |
| Molecular Formula |
C8H16N2O6S2-2.2[NA+]
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|---|---|
| Molecular Weight |
346.33204
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| Exact Mass |
346.024
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| CAS # |
76836-02-7
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| Related CAS # |
PIPES;5625-37-6
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| PubChem CID |
173553
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
20
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| Complexity |
370
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
GMHSTJRPSVFLMT-UHFFFAOYSA-L
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| InChi Code |
InChI=1S/C8H18N2O6S2.2Na/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16;;/h1-8H2,(H,11,12,13)(H,14,15,16);;/q;2*+1/p-2
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| Chemical Name |
disodium;2-[4-(2-sulfonatoethyl)piperazin-1-yl]ethanesulfonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~100 mg/mL (~288.74 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.8874 mL | 14.4371 mL | 28.8742 mL | |
| 5 mM | 0.5775 mL | 2.8874 mL | 5.7748 mL | |
| 10 mM | 0.2887 mL | 1.4437 mL | 2.8874 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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