| Size | Price | Stock | Qty |
|---|---|---|---|
| 25g |
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| 50g |
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| Other Sizes |
| Targets |
PIPES does not have a defined pharmacological target; it is a biological buffer. Its mechanism of action is purely chemical, functioning to resist changes in pH. It has a negligible capacity to bind divalent ions, making it suitable for studies involving metal ions. It is also used as a skin desquamation agent and a cream composition in personal care and cosmetic applications.
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|---|---|
| ln Vitro |
173 g of 1,4-piperazinediethanesulfonic acid are dissolved in 1 L of deionized water to create the pH PIPES buffer. Pellets of sodium hydroxide are added to the PIPES buffer to bring its pH down to 6.8. Lipid loss and artifacts caused by glutaraldehyde fixation can be avoided by using PIPES buffer [1].
In vitro, PIPES is used as a buffering agent in a wide array of biological applications, including as a component in media formulations, buffers for cell culture, and diagnostic reagent production. It has been documented to minimize lipid loss when buffering glutaraldehyde histology in plant and animal tissues. It is also used in protein purification and chromatography. |
| ln Vivo |
In vivo data for PIPES as a therapeutic agent are not applicable. Its primary role is as an in vitro reagent for maintaining pH in biochemical experiments. It is not administered for therapeutic purposes and has no established in vivo profile.
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| Enzyme Assay |
For in vitro biochemical assays, PIPES is used as a buffer component. A typical protocol involves preparing a PIPES buffer solution at a desired concentration (e.g., 10-100 mM) and adjusting the pH to the required value (e.g., pH 6.5-7.5). This buffer is then used in applications such as cell culture, protein purification, and histology.
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| Cell Assay |
For in vitro cell-based experiments, PIPES is used as a component of cell culture media to maintain pH. A standard protocol involves preparing a media solution containing PIPES at a concentration of 10-50 mM and using it to culture cells. The buffer helps maintain a stable pH, which is crucial for cell viability and experimental reproducibility.
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| Animal Protocol |
In vivo animal studies for PIPES are not applicable, as the compound is a buffer reagent used exclusively for in vitro applications. No animal studies have been conducted for this compound as a therapeutic agent.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for PIPES are not available, as the compound is not a drug and is not administered to living organisms. It is soluble in water (∼1 g/L at 100°C) and in 0.5 N NaOH (0.1 M).
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| Toxicity/Toxicokinetics |
PIPES is generally considered to be a low-toxicity buffer reagent. As a zwitterionic buffer, it is non-toxic and non-irritating at the concentrations used in biological research. Standard laboratory safety practices should be followed, including the use of personal protective equipment.
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| References | |
| Additional Infomation |
2,2'-piperazine-1,4-dimethylbis(ethanesulfonic acid) is a Goodyear buffer with a pKa of 6.8 at 20 °C. It is the conjugate acid of 2,2'-piperazine-1,4-dimethylbis(ethanesulfonic acid) salt.
PIPES (1,4-Piperazinediethanesulfonic acid) (CAS 5625-37-6) is a research-grade biochemical buffer, not an FDA-approved pharmaceutical drug. Its primary application is as a zwitterionic buffering agent for pH stabilization in biological and biochemical research, including cell culture, protein purification, and histology. |
| Molecular Formula |
C8H18N2O6S2
|
|---|---|
| Molecular Weight |
302.37
|
| Exact Mass |
670.067
|
| CAS # |
5625-37-6
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| Related CAS # |
PIPES disodium;76836-02-7;PIPES-d18;352534-95-3
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| PubChem CID |
79723
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| Appearance |
White to off-white solid powder
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| Density |
1.5±0.1 g/cm3
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| Melting Point |
>300 °C(lit.)
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| Index of Refraction |
1.566
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| LogP |
-4.21
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
18
|
| Complexity |
397
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C1CN(CCN1CCS(=O)(=O)O)CCS(=O)(=O)O
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| InChi Key |
IHPYMWDTONKSCO-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16)
|
| Chemical Name |
2-[4-(2-sulfoethyl)piperazin-1-yl]ethanesulfonic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 5 mg/mL (16.54 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: 2 mg/mL (6.61 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3072 mL | 16.5360 mL | 33.0721 mL | |
| 5 mM | 0.6614 mL | 3.3072 mL | 6.6144 mL | |
| 10 mM | 0.3307 mL | 1.6536 mL | 3.3072 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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