| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
Paclitaxel-d5 targets the same molecular target as paclitaxel: tubulin, the protein subunit of microtubules. Paclitaxel binds to the β-subunit of tubulin and stabilizes microtubule polymers, preventing their depolymerization. This stabilization interferes with the normal dynamic reorganization of the microtubule network essential for cell division, leading to mitotic arrest and apoptosis. As a deuterated internal standard, Paclitaxel-d5 does not exert pharmacological activity in assays but serves as a reference for quantification.
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|---|---|
| ln Vitro |
In vitro, Paclitaxel-d5 is used as an internal standard for the quantification of paclitaxel in biological samples using GC- or LC-MS. It has the same chemical properties as paclitaxel but with a different mass due to deuterium substitution, allowing for accurate quantification. Paclitaxel itself is a potent antineoplastic agent that stabilizes tubulin polymerization and induces mitotic arrest. Paclitaxel-d5 is not typically used for biological activity assays.
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| ln Vivo |
In vivo, Paclitaxel-d5 is used to investigate the metabolic pathways of paclitaxel in cancer treatment, helping researchers understand its efficacy, potential side effects, and interactions with other drugs. As a deuterated internal standard, it is administered alongside paclitaxel in pharmacokinetic studies to accurately measure drug concentrations. The compound is also used for analytical method development and quality control applications.
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| Enzyme Assay |
For in vitro analytical assays, Paclitaxel-d5 is spiked into biological samples (such as plasma, urine, or tissue homogenates) at known concentrations. Samples are extracted using liquid-liquid extraction or solid-phase extraction and analyzed by LC-MS or GC-MS. The ratio of Paclitaxel-d5 to paclitaxel is used to calculate the concentration of paclitaxel in the sample.
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| Cell Assay |
For cell-based assays, Paclitaxel-d5 is not typically used for biological activity studies. Instead, it is used as an internal standard in LC-MS analysis of cell lysates or culture media from cells treated with paclitaxel. The compound's deuterated nature allows for accurate quantification of paclitaxel uptake, metabolism, and efflux in cellular models.
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| Animal Protocol |
For in vivo pharmacokinetic studies, Paclitaxel-d5 is administered to animals alongside paclitaxel or as a separate dose. Blood samples are collected at various time points, and plasma is analyzed by LC-MS using Paclitaxel-d5 as the internal standard. Pharmacokinetic parameters such as half-life, Cmax, AUC, and clearance are calculated.
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| ADME/Pharmacokinetics |
Paclitaxel-d5 has a molecular weight of 858.94 and contains five deuterium atoms. It is soluble in organic solvents such as methanol, ethanol, and DMSO. Storage is recommended at -20°C for long-term stability. The compound is for research use only and is not intended for human or veterinary use.
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| Toxicity/Toxicokinetics |
Toxicological data for Paclitaxel-d5 are not separately reported, as it is a deuterated internal standard used at trace concentrations. Paclitaxel itself has well-characterized toxicity including myelosuppression, neuropathy, and hypersensitivity reactions. As a research compound, standard safety precautions should be followed when handling Paclitaxel-d5.
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| References |
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| Additional Infomation |
Paclitaxel-d5 is a deuterium-labeled form of paclitaxel used as an internal standard for the quantification of paclitaxel by GC- or LC-MS. It contains five deuterium atoms and has a molecular weight of 858.94. Paclitaxel-d5 is used in analytical method development, pharmacokinetic studies, and quality control applications. It is a research tool and is not approved for clinical use.
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| Molecular Formula |
C47H51NO14
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|---|---|
| Molecular Weight |
858.936963319778
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| Exact Mass |
858.362
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| CAS # |
1129540-33-5
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| Related CAS # |
Paclitaxel;33069-62-4
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| PubChem CID |
25191011
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| Appearance |
White to off-white solid powder
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| LogP |
4.31
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
14
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| Rotatable Bond Count |
14
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| Heavy Atom Count |
62
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| Complexity |
1790
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| Defined Atom Stereocenter Count |
11
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| SMILES |
[2H]C1=C(C(=C(C(=C1[2H])[2H])C(=O)N[C@@H](C2=CC=CC=C2)[C@H](C(=O)O[C@H]3C[C@]4([C@H]([C@H]5[C@@]([C@H](C[C@@H]6[C@]5(CO6)OC(=O)C)O)(C(=O)[C@@H](C(=C3C)C4(C)C)OC(=O)C)C)OC(=O)C7=CC=CC=C7)O)O)[2H])[2H]
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| InChi Key |
RCINICONZNJXQF-JQTCHTAZSA-N
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| InChi Code |
InChI=1S/C47H51NO14/c1-25-31(60-43(56)36(52)35(28-16-10-7-11-17-28)48-41(54)29-18-12-8-13-19-29)23-47(57)40(61-42(55)30-20-14-9-15-21-30)38-45(6,32(51)22-33-46(38,24-58-33)62-27(3)50)39(53)37(59-26(2)49)34(25)44(47,4)5/h7-21,31-33,35-38,40,51-52,57H,22-24H2,1-6H3,(H,48,54)/t31-,32-,33+,35-,36+,37+,38-,40-,45+,46-,47+/m0/s1/i8D,12D,13D,18D,19D
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| Chemical Name |
[(1S,2S,3R,4S,7R,9S,10S,12R,15S)-4,12-diacetyloxy-1,9-dihydroxy-15-[(2R,3S)-2-hydroxy-3-[(2,3,4,5,6-pentadeuteriobenzoyl)amino]-3-phenylpropanoyl]oxy-10,14,17,17-tetramethyl-11-oxo-6-oxatetracyclo[11.3.1.03,10.04,7]heptadec-13-en-2-yl] benzoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.1642 mL | 5.8211 mL | 11.6423 mL | |
| 5 mM | 0.2328 mL | 1.1642 mL | 2.3285 mL | |
| 10 mM | 0.1164 mL | 0.5821 mL | 1.1642 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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