| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| Other Sizes |
| Targets |
Moschamine targets mitochondrial superoxide production, inhibiting superoxide overproduction. It also inhibits COX-I and COX-II enzymes, reducing prostaglandin synthesis. Its anti-inflammatory and antioxidant activities contribute to its biological effects.
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|---|---|
| ln Vitro |
In vitro, Moschamine inhibits mitochondrial superoxide overproduction. It inhibits COX-I by 0.012% and COX-II by 54% in enzyme assays. It has antioxidant activity. It is a very potent compound with anti-inflammatory properties.
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| ln Vivo |
In vivo, Moschamine has bioavailability after oral administration. It has anti-inflammatory activity in animal models. Its antioxidant properties may protect against oxidative stress.
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| Enzyme Assay |
COX-I and COX-II inhibition assays use purified enzymes and colorimetric or fluorometric substrates. Moschamine is serially diluted and incubated with enzyme and substrate. Enzyme activity is measured by absorbance or fluorescence.
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| Cell Assay |
For antioxidant assays, cells are treated with Moschamine, and mitochondrial superoxide levels are measured using MitoSOX Red. ROS levels are measured using fluorescent probes.
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| Animal Protocol |
Animal models for inflammation include carrageenan-induced paw edema in rats. Moschamine is administered orally or intraperitoneally. Paw volume is measured by plethysmography.
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| Additional Infomation |
N-Feruloylserotonin is a hydroxyindole compound and an N-feruloyl derivative of serotonin. It is a plant metabolite. It belongs to the hydroxyindole, cinnamamide, phenol, aromatic ether, and secondary carboxamide classes. Its function is related to ferulic acid. It has been reported that thymol is present in Amberboa moschata, Centaurea arenaria, and other organisms with relevant data.
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| Molecular Formula |
C20H20N2O4
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|---|---|
| Exact Mass |
352.142
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| CAS # |
193224-22-5
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| Related CAS # |
N-Feruloylserotonin; 68573-23-9
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| PubChem CID |
5969616
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| Appearance |
White to off-white solid
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| Melting Point |
115 - 117 °C
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| Source |
Originated from plants: Compositae Amberboa moschata (L.) DC.
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| LogP |
3
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
26
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| Complexity |
498
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| Defined Atom Stereocenter Count |
0
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| SMILES |
COC1=C(C=CC(=C1)/C=C/C(=O)NCCC2=CNC3=C2C=C(C=C3)O)O
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| InChi Key |
WGHKJYWENWLOMY-XVNBXDOJSA-N
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| InChi Code |
InChI=1S/C20H20N2O4/c1-26-19-10-13(2-6-18(19)24)3-7-20(25)21-9-8-14-12-22-17-5-4-15(23)11-16(14)17/h2-7,10-12,22-24H,8-9H2,1H3,(H,21,25)/b7-3+
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| Chemical Name |
(E)-N-[2-(5-hydroxy-1H-indol-3-yl)ethyl]-3-(4-hydroxy-3-methoxyphenyl)prop-2-enamide
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| Synonyms |
N-feruloyl Serotonin; N-feruloylserotonin; Moschamine
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~100 mg/mL (~283.8 mM; with ultrasonication)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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