| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
Biological buffer; zwitterionic buffer
MOPSO does not have a specific biological target. It is a zwitterionic buffer used to maintain pH in biochemical and biological experiments. Good's buffers are characterized by high water solubility, low cell membrane permeability, consistent acid-base dissociation constants, low metal chelating capability, and high chemical stability. MOPSO is used in various applications including cell culture, enzyme assays, and protein purification. |
|---|---|
| ln Vitro |
The buffers 4-morpholinepropanesulfonic acid (MOPS) and 3-morpholino-2-hydroxypropanesulfonic acid (MOPSO) are useful biological zwitterionic buffers within the pH range of 6.5 to 7.9 and 6.2 to 7.6, respectively. The solubilities of these buffers were determined in binary mixtures (1,4-dioxane + water) and (ethanol + water) at T = 298.15 K by using the results of density measurements. It has been observed that MOPS induced liquid–liquid phase splitting for the mixtures of 40% to 90% (w/w) 1,4-dioxane in water. The two-liquid phase formation was visualized with disperse orange 25. The phase equilibrium boundaries, including the regions of one liquid, two liquids, (one liquid + one solid) and (two liquids + one solid), for the (MOPS + water + 1,4-dioxane) system have been determined experimentally at T = 298.15 K. The tie lines of the (liquid + liquid) equilibrium were also measured. The Othmer–Tobias and Bancroft equation were used to evaluate the reliability of the tie-line data. The binodal curve was fitted to an empirical equation and the effective excluded volume (EEV) model. The apparent free energies of transfer () of MOPS and MOPSO from water to 1,4-dioxane and ethanol solutions have been calculated from the solubility data. These values were compared with those of some related biological buffers (TRIS, TAPS, TAPSO, and TABS). Furthermore, we also calculated the contribution of transfer free energies () of –OH group from water to 1,4-dioxane and ethanol solutions[1].
MOPSO does not have direct in vitro biological activity as a pharmacological agent. Its value is as a buffer for biochemical and biological research. The compound maintains a stable pH environment for experiments without interfering with the biochemical processes being studied. It is used in buffer solutions for various in vitro applications including enzyme activity assays and protein purification. |
| ln Vivo |
MOPSO does not have in vivo biological activity as a pharmacological agent. It is not intended for administration to animals or humans as a therapeutic. Its value is as a laboratory reagent for biochemical research. The compound is used in buffer solutions for various in vitro and ex vivo applications.
|
| Enzyme Assay |
In vitro biochemical assays using MOPSO buffer are performed to maintain pH during experiments. The buffer is prepared at the desired pH within its effective range (6.2-7.6). MOPSO is used in enzyme activity assays and protein purification experiments. It has been used as a buffer component in charcoal yeast extract media and as a carrier electrolyte in capillary electrophoresis.
|
| Cell Assay |
Cell culture experiments using MOPSO are possible as its pH range (6.2-7.6) is close to physiological pH. It is commonly used in cell culture media. MOPSO serves as an effective buffering agent in cell culture media, contributing to a stable environment for cell growth and recombinant protein production.
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| Animal Protocol |
In vivo animal experiments are not applicable to MOPSO as it is a buffer reagent rather than a therapeutic agent. The compound is not intended for administration to animals. Its value is as a laboratory reagent for biochemical research.
|
| ADME/Pharmacokinetics |
Pharmacokinetic properties are not applicable to MOPSO as it is a buffer reagent rather than a therapeutic agent. The compound has a molecular weight of 225.26, a molecular formula of C7H15NO5S, and a pH range of 6.2-7.6. Solubility: H2O: 100 mg/mL (443.93 mM; Sonication is recommended). Storage: powder at -20°C for 3 years; in solvent at -80°C for 1 year.
|
| Toxicity/Toxicokinetics |
MOPSO is considered a safe buffer for laboratory use with minimal toxicity at typical working concentrations. Good's buffers are designed for minimal interference with biochemical processes. However, as with all chemical reagents, appropriate safety precautions should be followed. The compound is for research use only and is not for human therapeutic use.
|
| References |
[1]. Solubility and phase separation of 4-morpholinepropanesulfonic acid (MOPS), and 3-morpholino-2-hydroxypropanesulfonic acid (MOPSO) in aqueous 1,4-dioxane and ethanol solutions. The Journal of Chemical Thermodynamics. Volume 43, Issue 11, November 2011, Pages 1723-1730.
|
| Additional Infomation |
3-(n-morpholino)-2-hydroxypropanesulfonic acid is a member of the morpholino family of compounds.
MOPSO has CAS number 68399-77-9, molecular formula C7H15NO5S, and molecular weight 225.26. Synonyms: 2-hydroxy-3-morpholinopropane-1-sulfonic acid. It is a zwitterionic Good's buffer with a pH range of 6.2-7.6. Purity: 98%. Not for human use; for research purposes only. |
| Molecular Formula |
C7H15NO5S
|
|---|---|
| Molecular Weight |
225.2627
|
| Exact Mass |
225.067
|
| CAS # |
68399-77-9
|
| Related CAS # |
MOPSO sodium;79803-73-9
|
| PubChem CID |
109333
|
| Appearance |
Typically exists as White to off-white solid at room temperature
|
| Density |
1.4±0.1 g/cm3
|
| Melting Point |
275-280 °C (dec.)
|
| Index of Refraction |
1.540
|
| LogP |
-2.05
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
6
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
14
|
| Complexity |
254
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
S(C([H])([H])C([H])(C([H])([H])N1C([H])([H])C([H])([H])OC([H])([H])C1([H])[H])O[H])(=O)(=O)O[H]
|
| InChi Key |
NUFBIAUZAMHTSP-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C7H15NO5S/c9-7(6-14(10,11)12)5-8-1-3-13-4-2-8/h7,9H,1-6H2,(H,10,11,12)
|
| Chemical Name |
2-hydroxy-3-morpholin-4-ylpropane-1-sulfonic acid
|
| Synonyms |
2-Hydroxy-3-morpholinopropanesulfonic Acid; 2-Hydroxy-3-morpholinopropane-1-sulfonic acid; MOPSO, free acid; 3-Morpholino-2-hydroxypropanesulfonic acid; 3-(N-Morpholino)-2-hydroxypropanesulfonic Acid; 2-Hydroxy-4-morpholinepropanesulphonic acid;
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.4393 mL | 22.1966 mL | 44.3931 mL | |
| 5 mM | 0.8879 mL | 4.4393 mL | 8.8786 mL | |
| 10 mM | 0.4439 mL | 2.2197 mL | 4.4393 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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