| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
Biological buffer; zwitterionic buffer
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|---|---|
| ln Vitro |
The buffers 4-morpholinepropanesulfonic acid (MOPS) and 3-morpholino-2-hydroxypropanesulfonic acid (MOPSO) are useful biological zwitterionic buffers within the pH range of 6.5 to 7.9 and 6.2 to 7.6, respectively. The solubilities of these buffers were determined in binary mixtures (1,4-dioxane + water) and (ethanol + water) at T = 298.15 K by using the results of density measurements. It has been observed that MOPS induced liquid–liquid phase splitting for the mixtures of 40% to 90% (w/w) 1,4-dioxane in water. The two-liquid phase formation was visualized with disperse orange 25. The phase equilibrium boundaries, including the regions of one liquid, two liquids, (one liquid + one solid) and (two liquids + one solid), for the (MOPS + water + 1,4-dioxane) system have been determined experimentally at T = 298.15 K. The tie lines of the (liquid + liquid) equilibrium were also measured. The Othmer–Tobias and Bancroft equation were used to evaluate the reliability of the tie-line data. The binodal curve was fitted to an empirical equation and the effective excluded volume (EEV) model. The apparent free energies of transfer () of MOPS and MOPSO from water to 1,4-dioxane and ethanol solutions have been calculated from the solubility data. These values were compared with those of some related biological buffers (TRIS, TAPS, TAPSO, and TABS). Furthermore, we also calculated the contribution of transfer free energies () of –OH group from water to 1,4-dioxane and ethanol solutions[1].
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| References | |
| Additional Infomation |
3-(n-morpholino)-2-hydroxypropanesulfonic acid is a member of morpholines.
Solubility measurements[1] The values of the solubility of MOPS and MOPSO in water and aqueous 1,4-dioxane and ethanol solutions were determined with the aid of[1] Results and discussion[1] Density values, ρ, of MOPS and MOPSO in water and aqueous 1,4-dioxane and ethanol solutions (up to saturated conditions) at T = 298.15 K are reported in TABLE 1, TABLE 2, and for organic solvent concentrations, at 10% (w/w) intervals from (0 to 80)% w/w 1,4-dioxane or ethanol for MOPSO, from (0 to 90)% (w/w) ethanol for MOPS, and from (0 to 30)% w/w 1,4-dioxane for MOPS. The solubility limits of MOPS and MOPSO in water and in aqueous solutions of 1,4-dioxane and ethanol were obtained from density.[1] Conclusions[1] The values of the solubility of MOPS and MOPSO in the mixed solvents of (1,4-dioxane + water) and (ethanol + water) at T = 298.15 K have been determined by the results of density measurements. The solubilities generally decrease with an increase of the concentrations of 1,4-dioxane and ethanol. The solubilities of MOPS in water and in the binary solutions are higher than those of MOPSO, and decrease with increasing 1,4-dioxane and ethanol concentrations. MOPS show liquid–liquid phase separation at 40% [1]. |
| Molecular Formula |
C7H14NNAO5S
|
|---|---|
| Molecular Weight |
247.2445
|
| Exact Mass |
247.049
|
| CAS # |
79803-73-9
|
| Related CAS # |
MOPSO;68399-77-9
|
| PubChem CID |
23675131
|
| Appearance |
White to off-white solid powder
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
4
|
| Heavy Atom Count |
15
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| Complexity |
259
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
[Na].O=S(CC(CN1CCOCC1)O)(O)=O
|
| InChi Key |
WSFQLUVWDKCYSW-UHFFFAOYSA-M
|
| InChi Code |
InChI=1S/C7H15NO5S.Na/c9-7(6-14(10,11)12)5-8-1-3-13-4-2-8;/h7,9H,1-6H2,(H,10,11,12);/q;+1/p-1
|
| Chemical Name |
sodium;2-hydroxy-3-morpholin-4-ylpropane-1-sulfonate
|
| Synonyms |
MOPSO sodium salt; Sodium 2-hydroxy-3-morpholinopropane-1-sulfonate; 3-Morpholino-2-hydroxypropanesulfonic acid sodium salt; MOPSO (sodium); MFCD00070007; sodium;2-hydroxy-3-morpholin-4-ylpropane-1-sulfonate; sodium 2-hydroxy-3-(morpholin-4-yl)propane-1-sulfonate;
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~25 mg/mL (~101.12 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.0447 mL | 20.2233 mL | 40.4465 mL | |
| 5 mM | 0.8089 mL | 4.0447 mL | 8.0893 mL | |
| 10 mM | 0.4045 mL | 2.0223 mL | 4.0447 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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