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1mg |
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Other Sizes |
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Meclofenamic Acid (INF-4668; CI 583; INF4668; CI-583; Meclonax; Meclomen; Meclodium) is a potent anti-inflammatory agent of the NSAID (nonsteroidal anti-inflammatory drug) class. It acts as a dual COX-1/COX-2 inhibitor with IC50s of 40 nM and 50 nM, respectively. Meclofenamic Acid has been approved for use in the treatment of joint, muscular pain, arthritis and dysmenorrhea.
ln Vitro |
Meclofenamic acid inhibits FTO demethylation in a dose-response manner (0-100 μM, 24 hours) [1]. Meclofenamic acid suppresses prostaglandin synthesis by inhibiting cyclooxygenase, having an IC50 of about 1 μM [2]. Meclofenamic acid counteracts tissue reactions to certain prostaglandins and suppresses the production of 5-HETE and LTB4 from human neutrophils when induced by calcium ionophores [2].
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Cell Assay |
Western Blot Analysis[1]
Cell Types: HeLa Cell Tested Concentrations: 0, 12.5, 25, 50, 100 μM Incubation Duration: 24 h Experimental Results: Inhibited FTO demethylation in a dose-response manner and increased the level of m6A in cellular mRNA Targeting FTO. |
References |
[1]. Huang Y, et al. Meclofenamic acid selectively inhibits FTO demethylation of m6A over ALKBH5. Nucleic Acids Res. 2015 Jan;43(1):373-84.
[2]. Conroy MC, et al. Pharmacology, pharmacokinetics, and therapeutic use of meclofenamate sodium. Clin J Pain. 1991;7 Suppl 1:S44-8. [3]. Lee YT, et al. Inhibition of hKv2.1, a major human neuronal voltage-gated K+ channel, by meclofenamic acid. Eur J Pharmacol. 1999 Aug 13;378(3):349-56. [4]. Eleftheriou CG, et al. Meclofenamic acid improves the signal to noise ratio for visual responses produced by ectopicexpression of human rod opsin. Mol Vis. 2017 Jun 16;23:334-345. eCollection 2017. |
Molecular Formula |
C14H11CL2NO2
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Molecular Weight |
296.15
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Exact Mass |
295.01668
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CAS # |
644-62-2
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Related CAS # |
Meclofenamic acid sodium;6385-02-0;Meclofenamic acid sodium hydrate;67254-91-5;Meclofenamic acid-d4;1185072-18-7;Meclofenamic acid-13C6
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SMILES |
CC1=C(C(=C(C=C1)Cl)NC2=CC=CC=C2C(=O)O)Cl
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InChi Key |
SBDNJUWAMKYJOX-UHFFFAOYSA-N
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InChi Code |
InChI=1S/C14H11Cl2NO2/c1-8-6-7-10(15)13(12(8)16)17-11-5-3-2-4-9(11)14(18)19/h2-7,17H,1H3,(H,18,19)
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Chemical Name |
2-(2,6-dichloro-3-methylanilino)benzoic acid
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Synonyms |
INF-4668CI 583INF4668 CI-583Meclonax Meclomen Meclodium
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vitro) |
MEthanol : ~7.14 mg/mL (~24.11 mM)
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Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.3767 mL | 16.8833 mL | 33.7667 mL | |
5 mM | 0.6753 mL | 3.3767 mL | 6.7533 mL | |
10 mM | 0.3377 mL | 1.6883 mL | 3.3767 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.