| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
Purity: ≥98%
| Targets |
Metabotropic glutamate receptors 2 (mGluR2) and 3 (mGluR3). LY 541850 is a selective orthosteric mGlu2 agonist (IC50 = 0.161 μM) and an mGlu3 antagonist (IC50 = 0.038 μM).
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|---|---|
| ln Vitro |
LY 541850 is a selective orthosteric mGlu2 agonist with an IC50 of 0.161 μM and an mGlu3 antagonist with an IC50 of 0.038 μM. This dual activity allows for the study of the distinct roles of these two closely related glutamate receptors.
|
| ln Vivo |
In CD-1 CA1-SLM mice aged 10-16 weeks (EC50 42 nM), LY 541850 (ip; 10 mg/kg-300 mg/kg; 15 min ago) causes concentration-dependent inhibition of TAP-evoked fEPSP[1]. In male ICR mice, LY 541850 (ip; 10 mg/kg-300 mg/kg; 30 min ago) decreases in a dose-dependent manner the locomotor activity that is induced by amphetamine and phencyclidine [1].
LY 541850 can be used for its ability to improve cognitive function by modulating signaling pathways involved in memory and learning. Its in vivo effects are related to its modulation of glutamatergic neurotransmission. |
| Enzyme Assay |
In vitro assays for LY 541850 measure its ability to activate mGlu2 or inhibit mGlu3. This is typically done using cells expressing the recombinant receptors and measuring downstream signaling, such as calcium mobilization or cAMP accumulation.
|
| Cell Assay |
Cellular assays for LY 541850 involve using cells that recombinantly express human mGlu2 or mGlu3 receptors. The compound's ability to modulate receptor-mediated signaling pathways is measured to confirm its agonist and antagonist activities.
|
| Animal Protocol |
In vivo animal models for LY 541850 would be related to its potential use in improving cognitive function. This could involve using rodent models of learning and memory to assess the compound's effects on cognitive performance.
|
| References |
| Molecular Formula |
C10H15NO4
|
|---|---|
| Molecular Weight |
213.23
|
| Exact Mass |
199.084
|
| CAS # |
852679-76-6
|
| Related CAS # |
(±)-LY395756;852679-66-4
|
| PubChem CID |
11310142
|
| Appearance |
Typically exists as solid at room temperature
|
| LogP |
-2.8
|
| tPSA |
101
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
14
|
| Complexity |
316
|
| Defined Atom Stereocenter Count |
5
|
| SMILES |
C[C@@H]1C[C@]([C@H]2[C@@H]1[C@@H]2C(=O)O)(C(=O)O)N
|
| InChi Key |
BQVZICWQBFTOJX-QNDZYWSYSA-N
|
| InChi Code |
InChI=1S/C9H13NO4/c1-3-2-9(10,8(13)14)6-4(3)5(6)7(11)12/h3-6H,2,10H2,1H3,(H,11,12)(H,13,14)/t3-,4+,5+,6+,9+/m1/s1
|
| Chemical Name |
(1S,2S,4R,5R,6S)-2-Amino-4-methylbicyclo[3.1.0]hexane2,6-dicarboxylic acid
|
| Synonyms |
LY-541850; LY 541850; LY541850
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.6898 mL | 23.4489 mL | 46.8977 mL | |
| 5 mM | 0.9380 mL | 4.6898 mL | 9.3795 mL | |
| 10 mM | 0.4690 mL | 2.3449 mL | 4.6898 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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