| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| 1g | |||
| Other Sizes |
| Targets |
Levomefolate sodium targets folate-dependent metabolic pathways. It is the predominant folate transport form in blood under physiological conditions. As the active form of folic acid, it serves as a cofactor for enzymes involved in one-carbon metabolism, including methionine synthase, which converts homocysteine to methionine, and enzymes involved in DNA synthesis and repair.
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| ln Vitro |
In vitro, Levomefolate sodium is the biologically active form of folate. It is involved in the synthesis of DNA, the cysteine cycle, and the regulation of homocysteine. It is used as a folate supplement in cell culture media to support cell growth and proliferation.
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| ln Vivo |
In vivo, Levomefolate sodium is an orally active and blood-brain barrier-penetrating form of folate. It has been studied as an adjunctive treatment for individuals with major depressive disorder who exhibit partial or no response to traditional antidepressants such as SSRIs or SNRIs. It can be used as a food supplement for folic acid and in the research of neural tube defect diseases.
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| Enzyme Assay |
Levomefolate sodium is evaluated in cell-free assays as a reference standard for folate analysis. Its purity and identity can be confirmed using HPLC and NMR analysis. The compound is used in biochemical assays to study the activity of folate-dependent enzymes, such as methionine synthase and methylenetetrahydrofolate reductase.
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| Cell Assay |
Levomefolate sodium is assessed in cell-based assays using various cell lines. Cells are cultured in media containing Levomefolate sodium, and its effects on cell proliferation, DNA synthesis, and homocysteine levels are measured. It is used to study the role of folate in cellular metabolism and gene expression.
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| Animal Protocol |
Levomefolate sodium is administered orally in animal models and in clinical studies. It is used as a dietary supplement to study the effects of folate on various physiological processes, including neural tube development, cardiovascular health, and cognitive function.
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| ADME/Pharmacokinetics |
Levomefolate sodium is orally active and can cross the blood-brain barrier. It has a molecular weight of 503.42 and a molecular formula of C20H23N7Na2O6. Its CAS number is 1423663-76-6. The compound is soluble in water and should be stored under recommended conditions.
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| Toxicity/Toxicokinetics |
No detailed toxicity data is available for Levomefolate sodium. It is a form of folate and is generally considered safe as a dietary supplement. The compound is for research use only and is not intended for human therapeutic use.
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| Additional Infomation |
Levomefolate sodium is also known as L-5-MTHF sodium and (6S)-5-methyltetrahydrofolate sodium. It is a dietary supplement and a research tool for studying folate metabolism and related disorders. The compound is not approved for clinical use.
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| Molecular Formula |
C20H23N7NA2O6
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|---|---|
| Molecular Weight |
503.419465303421
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| Exact Mass |
503.151
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| CAS # |
1423663-76-6
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| Related CAS # |
31690-09-2 (free acid);151533-22-1 (calcium);419563-18-1 (calcium pentahydrate);1423663-76-6 (sodium); 1429498-11-2 (magnesium);
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| PubChem CID |
135565435
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| Appearance |
Typically exists as solid at room temperature
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
10
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
35
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| Complexity |
854
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| Defined Atom Stereocenter Count |
2
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| SMILES |
[Na+].[Na+].O=C1C2=C(N=C(N)N1)NC[C@H](CNC1C=CC(C(N[C@H](C(=O)[O-])CCC(=O)[O-])=O)=CC=1)N2C
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| InChi Key |
KKIWVYLOTHCGRV-NJHZPMQHSA-L
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| InChi Code |
InChI=1S/C20H25N7O6.2Na/c1-27-12(9-23-16-15(27)18(31)26-20(21)25-16)8-22-11-4-2-10(3-5-11)17(30)24-13(19(32)33)6-7-14(28)29;;/h2-5,12-13,22H,6-9H2,1H3,(H,24,30)(H,28,29)(H,32,33)(H4,21,23,25,26,31);;/q;2*+1/p-2/t12-,13-;;/m0../s1
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| Chemical Name |
disodium;(2S)-2-[[4-[[(6S)-2-amino-5-methyl-4-oxo-3,6,7,8-tetrahydropteridin-6-yl]methylamino]benzoyl]amino]pentanedioate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.9864 mL | 9.9321 mL | 19.8641 mL | |
| 5 mM | 0.3973 mL | 1.9864 mL | 3.9728 mL | |
| 10 mM | 0.1986 mL | 0.9932 mL | 1.9864 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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