| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| Other Sizes |
| Targets |
L-Leucine-d2 targets the same biological pathways as unlabeled L-leucine, an essential branched-chain amino acid (BCAA) that activates the mTOR signaling pathway. mTOR is a key regulator of cell growth, protein synthesis, and metabolism. L-leucine stimulates mTOR activation, promoting protein synthesis and cell growth. The labeled compound is primarily used as a tracer in mass spectrometry and metabolic studies rather than for therapeutic targeting.
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| ln Vitro |
Drug compounds have been modified to include stable heavy isotopes of hydrogen, carbon, and other elements. These isotopes are mostly utilized as quantitative tracers during the drug development process. 1].
L-Leucine-d2 is a deuterium-labeled amino acid used as an internal standard in mass spectrometry. L-leucine, the unlabeled parent compound, is an essential branched-chain amino acid that activates the mTOR signaling pathway. The compound is involved in protein synthesis and various metabolic processes. The labeled compound allows for the quantification and tracking of leucine metabolism in cells and tissues. L-Leucine is also important for muscle protein synthesis and metabolic regulation. |
| ln Vivo |
In vivo, L-leucine is an essential amino acid that is absorbed from the diet and incorporated into proteins. L-Leucine-d2 can be administered to animals or humans as a tracer to study leucine metabolism and protein turnover. The labeled compound is detected by mass spectrometry, allowing for the quantification of leucine flux in various tissues. L-leucine activates the mTOR signaling pathway in vivo, promoting protein synthesis and muscle growth.
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| Enzyme Assay |
In vitro assays for L-Leucine-d2 typically involve its use as an internal standard in mass spectrometry-based metabolic studies. The compound is added to biological samples or cell culture media at known concentrations. Samples are analyzed by LC-MS/MS to quantify leucine and its metabolites. Standard protocols involve protein precipitation, derivatization (for GC-MS), and mass spectrometric analysis. The compound is soluble in water (12.5 mg/mL) and PBS (pH 7.2).
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| Cell Assay |
In vitro cellular assays for L-Leucine-d2 typically involve culturing cells in media containing the labeled amino acid. Cells are incubated with the labeled leucine for various time periods to study leucine metabolism and mTOR signaling. Cell extracts are prepared and analyzed by mass spectrometry to quantify leucine and its metabolites. The labeled compound allows for the tracking of leucine metabolism and the study of mTOR pathway activation.
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| Animal Protocol |
In vivo animal studies for L-Leucine-d2 typically involve administering the labeled compound to mice or rats via oral gavage, intravenous injection, or dietary supplementation. Blood, urine, and tissue samples are collected at various time points and analyzed by mass spectrometry to track leucine metabolism. The labeled compound allows for the quantification of leucine flux and protein synthesis rates in different tissues. Doses are determined based on the specific study objectives.
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| ADME/Pharmacokinetics |
L-Leucine-d2 has a molecular weight of 133.19 g/mol and formula C6H11D2NO2. The compound is soluble in water (12.5 mg/mL) and PBS (pH 7.2). Recommended storage is at -20°C for powder. Detailed PK parameters would be similar to those of unlabeled L-leucine, which is actively transported and metabolized in the body. The compound is stable under recommended storage conditions.
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| Toxicity/Toxicokinetics |
Toxicity data for L-Leucine-d2 are similar to those of unlabeled L-leucine, which is an essential amino acid with low toxicity. L-leucine is generally recognized as safe when consumed as a dietary supplement. The labeled compound is intended for research use only and is not approved for human therapeutic applications. Standard safety precautions should be taken when handling the compound.
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| References |
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| Additional Infomation |
L-Leucine-d2 (CAS 362049-59-0) is a deuterium-labeled form of L-leucine. It has a molecular weight of 133.19 g/mol and formula C6H11D2NO2. L-leucine is an essential branched-chain amino acid that activates the mTOR signaling pathway. The labeled compound is used as an internal standard in mass spectrometry and metabolic studies. It is also known as L-leucine-d2 and 2H-labeled leucine. The compound is not approved for clinical use.
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| Molecular Formula |
C6H11D2NO2
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|---|---|
| Molecular Weight |
133.19
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| Exact Mass |
133.107
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| CAS # |
362049-59-0
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| Related CAS # |
L-Leucine;61-90-5
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| PubChem CID |
87293673
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| Appearance |
White to off-white solid powder
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| Density |
1.0±0.1 g/cm3
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| Boiling Point |
225.8±23.0 °C at 760 mmHg
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| Flash Point |
90.3±22.6 °C
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| Vapour Pressure |
0.0±0.9 mmHg at 25°C
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| Index of Refraction |
1.463
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| LogP |
0.73
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
9
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| Complexity |
101
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| Defined Atom Stereocenter Count |
1
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| SMILES |
[2H]C([2H])([C@@H](C(=O)O)N)C(C)C
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| InChi Key |
ROHFNLRQFUQHCH-YVKXTFNSSA-N
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| InChi Code |
InChI=1S/C6H13NO2/c1-4(2)3-5(7)6(8)9/h4-5H,3,7H2,1-2H3,(H,8,9)/t5-/m0/s1/i3D2
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| Chemical Name |
(2S)-2-amino-3,3-dideuterio-4-methylpentanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 7.5081 mL | 37.5404 mL | 75.0807 mL | |
| 5 mM | 1.5016 mL | 7.5081 mL | 15.0161 mL | |
| 10 mM | 0.7508 mL | 3.7540 mL | 7.5081 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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