| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 50mg | |||
| Other Sizes |
| Targets |
α-glucosidase (from Saccharomyces cerevisiae) – the compound showed binding affinity to the enzyme, with enhancement factors of 9.45%, 12.64%, and 13.76% at α-glucosidase concentrations of 0.75, 1.50, and 2.25 U/mL, respectively (these values represent the specific binding ratio of the compound to the target enzyme). [1]
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|---|---|
| ln Vitro |
In an ultrafiltration LC-ESI-MS affinity assay, Iristectorigenin A demonstrated specific binding to α-glucosidase. The binding capacity, expressed as the enhancement factor (percentage of compound specifically bound relative to total compound in incubation), increased with higher enzyme concentrations: 9.45% at 0.75 U/mL, 12.64% at 1.50 U/mL, and 13.76% at 2.25 U/mL. This binding affinity was comparable to that of tectorigenin and irigenin, but lower than that of irisflorentin (which showed enhancement factors of 28.14%, 31.94%, and 36.75% under the same conditions). The structure-activity relationship analysis suggested that the presence of hydroxyl groups at positions R1, R2, and R3 (referring to the isoflavonoid skeleton) influences the inhibitory activity, and loss of these hydroxyls significantly reduces activity. Iristectorigenin A has similar substitution patterns on ring A but different substitution on ring C compared to tectorigenin and irigenin, leading to almost the same level of binding affinity to α-glucosidase. [1]
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| Enzyme Assay |
The ultrafiltration LC-MS screening for α-glucosidase inhibitors was performed as follows: 20 μL of B. chinensis extract (100 mg/mL) was mixed with 80 μL of α-glucosidase (10 U/mL, dissolved in ammonium acetate buffer at pH 6.8) at varying concentrations (0.75, 1.50, and 2.25 U/mL) and 100 μL of ammonium acetate buffer (pH 6.8) to a total volume of 200 μL. The mixture was incubated at 37°C for 40 minutes. Then, ultrafiltration was carried out using a centrifuge with a 30-kDa molecular weight cutoff ultrafiltration membrane filter at 12,000 rpm for 10 minutes at 25°C. The filter was washed three times by centrifugation with 100 mL aliquots of ammonium acetate buffer (pH 6.8) to remove unbound low-mass molecules. The bound isoflavonoids were released by adding 100 mL of methanol solution (50:50 v/v, pH 3.1), followed by centrifugation at 12,000 rpm for 10 minutes. This release step was repeated three times. A control group experiment with denatured enzymes was also carried out before each screening experiment. The ultrafiltrate samples were directly injected into the LC-MS system for analysis. The mobile phase consisted of water (solvent A) and acetonitrile (solvent B) with a gradient program at a flow rate of 0.5 mL/min. ESI-MS in positive mode was used for data collection. The binding of a ligand to α-glucosidase was calculated using the equation: enhancement factor (%) = (A1/A2) × 100%, where A1 is the amount of compound specifically bound to α-glucosidase and A2 is the total amount of compound in the reaction. [1]
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| References |
:J Sep Sci. 2017 Jun;40(12):2565-2574.
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| Additional Infomation |
It has been reported that iris flowers, ash grass, and other organisms with available data contain irisin A.
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| Molecular Formula |
C17H14O7
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|---|---|
| Molecular Weight |
330.292
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| Exact Mass |
330.074
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| Elemental Analysis |
C, 61.82 H, 4.27 O, 33.91
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| CAS # |
39012-01-6
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| PubChem CID |
5491637
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| Appearance |
White to light yellow solid powder
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| Density |
1.483g/cm3
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| Boiling Point |
619ºC at 760 mmHg
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| Melting Point |
237-238 ºC
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| Flash Point |
232ºC
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| Index of Refraction |
1.67
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| Source |
Originated from plants: Iridaceae Belamcanda chinensis (Linn.) Redouté
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| LogP |
2.594
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
24
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| Complexity |
505
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O1C([H])=C(C2C([H])=C([H])C(=C(C=2[H])OC([H])([H])[H])O[H])C(C2C(=C(C(=C([H])C1=2)O[H])OC([H])([H])[H])O[H])=O
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| InChi Key |
CCRPIWFQMLICCY-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C17H14O7/c1-22-12-5-8(3-4-10(12)18)9-7-24-13-6-11(19)17(23-2)16(21)14(13)15(9)20/h3-7,18-19,21H,1-2H3
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| Chemical Name |
5,7-dihydroxy-3-(4-hydroxy-3-methoxyphenyl)-6-methoxychromen-4-one
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| Synonyms |
Iristectorigenin A
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~125 mg/mL (~378.5 mM; with ultrasonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.08 mg/mL (6.30 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (20.8 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (6.30 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (20.8 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0276 mL | 15.1382 mL | 30.2764 mL | |
| 5 mM | 0.6055 mL | 3.0276 mL | 6.0553 mL | |
| 10 mM | 0.3028 mL | 1.5138 mL | 3.0276 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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