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Purity: ≥98%
Flumequine (formerly R-802; R 802; R802) is a synthetic quinolone, 1st generation and broad-spectrum chemotherapeutic antibiotic that was once used to treat bacterial infections but has been removed from market. It functions by 15 μM inhibiting topoisomerase II. Both bacterial gyrase and eukaryotic topoisomerase II, which catalyzes the double-strand DNA breakage reaction, are inhibited by flumequine. The inhibitory effects of FL on topoisomerase II are greater than the influence on bacterial gyrase. Twelve clinical isolates of A. salmonicida have minimum inhibitory concentrations (MICs) for memequine ranging from 0.06 μg/mL to 32 μg/mL.
| Targets |
Topoisomerase II ( IC50 = 15 μM ); Quinolone
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| Cell Assay |
The Chinese hamster lung cell line CHL/IU is routinely cultured in monolayer form at 37°C in a 5% CO2 environment using Dulbecco's modified MEM medium supplemented with 10% fetal bovine serum. For one hour, exponentially growing cells are exposed to a solution of flumequine (R-802) dissolved in DMSO. The dose range is selected to extract both severely damaged and undamaged cells. After being treated with Flumequine (R-802) the cells are embedded in 1% saline-dissolved GP42 agarose. For every dosage, the number and viability of cells are ascertained.
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| Animal Protocol |
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| References |
| Molecular Formula |
C14H12FNO3
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| Molecular Weight |
261.25
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| Exact Mass |
261.08
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| Elemental Analysis |
C, 64.36; H, 4.63; F, 7.27; N, 5.36; O, 18.37
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| CAS # |
42835-25-6
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| Appearance |
Solid powder
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| SMILES |
CC1CCC2=C3N1C=C(C(=O)C3=CC(=C2)F)C(=O)O
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| InChi Key |
DPSPPJIUMHPXMA-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C14H12FNO3/c1-7-2-3-8-4-9(15)5-10-12(8)16(7)6-11(13(10)17)14(18)19/h4-7H,2-3H2,1H3,(H,18,19)
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| Chemical Name |
7-fluoro-12-methyl-4-oxo-1-azatricyclo[7.3.1.05,13]trideca-2,5,7,9(13)-tetraene-3-carboxylic acid
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| Synonyms |
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.8278 mL | 19.1388 mL | 38.2775 mL | |
| 5 mM | 0.7656 mL | 3.8278 mL | 7.6555 mL | |
| 10 mM | 0.3828 mL | 1.9139 mL | 3.8278 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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