| Size | Price | Stock | Qty |
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| 500mg |
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| 1g |
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| 5g |
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| 10g |
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Purity: ≥98%
Ellagic acid (Elagostasine, Gallogen; Alizarine Yellow; Benzoaric acid; CCRIS-774; HSDB-7574; Lagistase), the dilactone of hexahydroxydiphenic acid, is a naturally occuring phenol and an antioxidant found in numerous fruits and vegetables. It possesses antioxidant and antiproliferative qualities in several in vitro and small animal models. Research on Ellagic acid's possible health benefits has been spurred by its antioxidant and antiproliferative qualities. Because it can directly prevent some carcinogens, such as nitrosamines and polycyclic aromatic hydrocarbons, from binding to DNA, Ellagic acid may have antiproliferative effects.
| Targets |
CK2 ( IC50 = 40 nM )
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| ln Vitro |
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| ln Vivo |
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| Enzyme Assay |
The CK2 and CK1 phosphorylation tests are conducted at 37°C with increasing concentrations of each inhibitor (Ellagic acid) in a final volume of 25 µL that contains 50 mM, pH 7.5 Tris-HCl, 100 mM NaCl, 12 mM MgCl2, and 0.02 mM [33P-ATP] (500-1000 cpm/pmol), unless otherwise specified. For CK1 and CK2, the phosphorylatable substrates are RRKHAAIGDDDDAYSITA (200 µM) and RRRADDSDDDDD (100 µM), respectively, synthetic peptide substrates. The kinase was added first, and after 10 minutes, the reaction was stopped by adding 5 µL of 0.5 M orthophosphoric acid. This was done before aliquots were placed onto phosphocellulose filters. After the radiolabeled samples are separated by SDS-PAGE, filters are cleaned in a 75 mM phosphoric acid substrate. The tyrosine kinase activities of DYRK1A are measured using the peptide RRRFRPASPLRGPPK[1].
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| Cell Assay |
MTT assay is used to determine ALCL cell viability. In short, 12 hours before adding ellagic acid, 0.1 × 105 cells are seeded onto 96-well microculture plates. The cells are cultured in 200 µL of full RPMI-1640 medium for 48 hours, either with or without the medication (elagic acid), following standard tissue-culture procedures. Next, add 20 µL of MTT solution (5 mg/mL) to the cell suspension and let it sit for 4 hours. 150 µL of DMSO is used to dissolve the intracellular formazan crystals, and the optical density—which is determined at 540 nm using a spectrophotometer—represents the average (± SD) of three replicate cultures[1].
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| Animal Protocol |
Fifty adult male Sprague-Dawley rats are split into five groups at random, which are as follows: As a vehicle and standard control, Group (1) is given oral corn oil. Doxorubicin (DOX) injections (5 mg/kg, i.p.) are given to Group (2) twice a week for a duration of 14 days. For a duration of 14 days, Group 3 is administered Ellagic acid (10 mg/kg, p.o.; daily) and DOX (5 mg/kg, i.p.) twice a week. For 14 days, rosmarinic acid (RA; 75 mg/kg, p.o.; daily) and DOX (5 mg/kg, i.p.) are given to Group 4. For a duration of 14 days, Group 5 is administered Ellagic acid (10 mg/kg, p.o.; daily) along with RA (75 mg/kg, p.o.; daily) and a DOX injection (5 mg/kg, i.p.) twice a week[2].
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| References |
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| Molecular Formula |
C14H6O8
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| Molecular Weight |
302.19
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| Exact Mass |
302.01
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| Elemental Analysis |
C, 55.64; H, 2.00; O, 42.35
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| CAS # |
476-66-4
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| Related CAS # |
133039-73-3;314041-08-2
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| Appearance |
Solid powder
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| SMILES |
C1=C2C3=C(C(=C1O)O)OC(=O)C4=CC(=C(C(=C43)OC2=O)O)O
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| InChi Key |
AFSDNFLWKVMVRB-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C14H6O8/c15-5-1-3-7-8-4(14(20)22-11(7)9(5)17)2-6(16)10(18)12(8)21-13(3)19/h1-2,15-18H
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| Chemical Name |
6,7,13,14-tetrahydroxy-2,9-dioxatetracyclo[6.6.2.04,16.011,15]hexadeca-1(15),4,6,8(16),11,13-hexaene-3,10-dione
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| Synonyms |
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
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| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3092 mL | 16.5459 mL | 33.0918 mL | |
| 5 mM | 0.6618 mL | 3.3092 mL | 6.6184 mL | |
| 10 mM | 0.3309 mL | 1.6546 mL | 3.3092 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
| NCT Number | Recruitment | interventions | Conditions | Sponsor/Collaborators | Start Date | Phases |
| NCT04011618 | Active Recruiting |
Drug: Ellagic Acid / Pomegranate Extract Drug: Placebo oral capsule |
Metabolic Syndrome | University of Guadalajara | September 17, 2019 | Phase 2 |
| NCT04066816 | Completed | Other: Walnuts | Colo-rectal Cancer Colon Cancer |
UConn Health | May 20, 2019 | Not Applicable |
| NCT04066816 | Completed | Other: Walnuts | Colo-rectal Cancer Colon Cancer |
UConn Health | May 20, 2019 | Not Applicable |
| NCT03713164 | Completed | Dietary Supplement: Pomegranate Juice Dietary Supplement: Ellagic Acid |
Healthy | University of California, Los Angeles |
February 22, 2018 | Not Applicable |
| NCT02263378 | Completed | Drug: ellagic acid + annona muricata |
HPV Infection | University of Messina | September 2014 | Not Applicable |
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