| Size | Price | Stock | Qty |
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| 100mg |
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| 250mg |
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| 500mg |
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| 1g |
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| 2g |
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| 5g | |||
| Other Sizes |
Purity: ≥98%
| Targets |
No pharmacological target; it is an enzymatic tool. Driselase acts on plant cell wall components: cellulose, hemicellulose (xylan, glucuronoxylan), pectin, and β-1,3-glucan (callose). The combined action of these enzymes breaks down the rigid cell wall, releasing individual cells (protoplasts) while maintaining viability. It is also used to digest fungal cell walls for preparation of protoplasts from filamentous fungi.
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| ln Vitro |
Driselase is a crude lytic enzyme preparation that contains a spectrum of activities, including cellulase, hemicellulase, and pectinase, which work synergistically to degrade plant cell walls. Its primary application is in the generation of protoplasts.
In vitro, Driselase is assessed for its ability to release protoplasts from plant tissues. The enzymatic activity is measured by the release of reducing sugars from substrates like carboxymethylcellulose (cellulase), xylan (xylanase), or pectin (pectinase). One unit typically releases 1 µmol of glucose equivalent per minute at pH 5.0 and 40°C. The optimal pH range is 4.5–5.5, and temperature optimum is 37–45°C. Activity is inhibited by heavy metals and high salt concentrations. |
| ln Vivo |
Not applicable as a therapeutic agent. Driselase is used only in research and industrial applications. In plant protoplast isolation, it is applied at concentrations of 0.5–2% (w/v) in osmotically balanced buffers (e.g., 0.4 M mannitol) for 2–4 hours at 25–30°C. The resulting protoplasts are used for transformation, fusion, and physiological studies. In fungal research, it helps generate protoplasts for genetic manipulation.
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| Enzyme Assay |
Cell-free activity assays: incubate 1 mL of Driselase solution (1 mg/mL in 50 mM sodium acetate buffer, pH 5.0) with 1% substrate (e.g., carboxymethylcellulose, xylan, or pectin) at 40°C for 30 min. Terminate by boiling, then measure reducing sugars using DNS reagent and spectrophotometry at 540 nm. One unit is defined as the amount of enzyme that releases 1 µmol of glucose per minute. Alternatively, use viscometry or turbidimetry to assess polymer degradation.
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| Cell Assay |
Not used in cell-based pharmacological assays. In plant cell biology, tissues (e.g., tobacco leaves) are cut into small pieces, vacuum-infiltrated with Driselase solution (1% w/v in mannitol buffer), and incubated on a shaker for 2–3 h. Protoplasts are released and collected by filtration and centrifugation. Viability is assessed by FDA staining. Protoplasts can then be cultured for transformation or regeneration studies.
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| Animal Protocol |
Not used in animal models. Driselase is a research reagent for plant and fungal studies. No in vivo animal experiments are conducted with this compound, as it is not biologically active in mammalian systems. Its use is confined to in vitro tissue culture and biochemical assays.
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| ADME/Pharmacokinetics |
Driselase is a crude powder with variable composition; exact MW not defined. Appearance: light brown to beige powder. Solubility: soluble in water and dilute buffers. Storage: store at 2-8°C in a dry, airtight container; stable for at least 6 months. Activity may decrease over time; store aliquots at -20°C for long-term. For use, dissolve in buffer with gentle stirring; insoluble residue may be removed by centrifugation.
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| Toxicity/Toxicokinetics |
No toxicity concerns for humans at the concentrations used in the laboratory. As an enzyme preparation, it may contain allergens; avoid inhalation of dust. Standard safety precautions (gloves, lab coat, goggles) should be taken. It is not intended for injection or ingestion.
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| References | |
| Additional Infomation |
This study evaluated the effects of various cell wall-degrading enzymes and protoplast culture media on protoplast production in Fusarium verticillioides. Among the enzymes tested, driselase at a concentration of 12.5 mg/ml produced the highest number of protoplasts in 1 M KCl protoplast medium. Secondly, lyase at a concentration of 10 mg/ml also produced the highest number of protoplasts in 1.2 M MgSO4 protoplast medium. More interestingly, the simultaneous use of driselase at a concentration of 12.5 mg/ml and lyase at a concentration of 10 mg/ml in 1 M KCl medium exhibited a synergistic effect on protoplast formation. Germinating conidia of Fusarium are the most susceptible fungal material for protoplast production. The addition of 1.2 M sucrose to the regeneration medium maximally promoted protoplast regeneration. This study recommends adding driselase (12.5 mg/ml) and lyase (10 mg/ml) to 1 M KCl protoplast medium and using germinating conidia of F. verticillioides to maximize protoplast production; 1.2 M sucrose is the optimal permeabilizer for protoplast regeneration. [1]
Not a drug; it is a research enzyme preparation widely used in plant biotechnology, especially for creating transgenic plants, somatic hybridization, and studying cell wall architecture. It is also used in the food industry for clarification of fruit juices and in waste treatment. No clinical trials are relevant. |
| CAS # |
85186-71-6
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|---|---|
| PubChem CID |
168010183
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| Appearance |
Typically exists as solid at room temperature
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
24
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| Heavy Atom Count |
71
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| Complexity |
2340
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| Defined Atom Stereocenter Count |
0
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
Soluble in water (~1 mg/mL), yielding a clear to slightly turbid solution upon dissolution. Alternatively, dissolve according to specific references.
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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