| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| 100mg | |||
| Other Sizes |
| Targets |
Not applicable; this is an analytical tracer. Unlabeled D-ribose is a pentose sugar that serves as the sugar moiety of ATP, NADH, FADH2, and RNA. It is involved in the pentose phosphate pathway (PPP), where it is produced from glucose-6-phosphate via the oxidative branch.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
D-Ribose-d2 has the same biological activity as unlabeled D-ribose. D-ribose enhances ATP resynthesis in cardiac and skeletal muscle following ischemia or strenuous exercise, promotes energy recovery, and is used as a dietary supplement for chronic fatigue syndrome and myocardial energy metabolism. |
| ln Vivo |
Not applicable as a drug; D-Ribose-d2 is a tracer. Unlabeled D-ribose (5-15 g/day) has been shown to improve diastolic function in patients with coronary artery disease and reduce symptoms of fibromyalgia and chronic fatigue syndrome in small clinical studies.
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| Enzyme Assay |
D-Ribose-d2 is spiked into biological samples (plasma, urine, tissue lysates) at known concentrations (1-50 microM). Samples are deproteinized with methanol/acetonitrile, derivatized if necessary, and analyzed by LC-MS/MS or GC-MS to quantify endogenous D-ribose by isotope dilution.
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| Cell Assay |
Primary cardiomyocytes or myoblasts (C2C12 cells) are treated with D-Ribose-d2 (1-20 mM) for 1-24 hours under normal or ATP-depleting conditions (e.g., 2-deoxyglucose). Cells are harvested, and the 13C/2H enrichment in ATP, ADP, and AMP is measured by LC-MS/MS to determine rates of ATP synthesis via the salvage pathway.
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| Animal Protocol |
Mice or rats are administered D-Ribose-d2 (50-500 mg/kg) by intraperitoneal injection or oral gavage. Blood is collected at multiple time points (0-6 hours), and hearts or skeletal muscles are harvested. The 2H enrichment in ribose-5-phosphate, ATP, and RNA is quantified by LC-MS/MS.
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| ADME/Pharmacokinetics |
Not available for D-Ribose-d2. Unlabeled D-ribose has an oral bioavailability of 20-40% in humans, with a plasma half-life of approximately 1-2 hours. It is rapidly taken up by tissues (especially heart, skeletal muscle, and liver) and is primarily metabolized to ribose-5-phosphate via ribokinase.
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| Toxicity/Toxicokinetics |
No toxicity; D-ribose is generally recognized as safe (GRAS) as a dietary supplement. At high doses (>15 g/day), it may cause mild gastrointestinal discomfort (diarrhea, nausea). The deuterium-labeled version is safe at tracer levels.
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| References | |
| Additional Infomation |
D-Ribose-d2 is for research use only, not FDA-approved. Unlabeled D-ribose is available as a dietary supplement. The d2-labeled version is a valuable analytical internal standard for LC-MS/MS and NMR studies of ribose metabolism, energy metabolism disorders, and for quantifying RNA turnover in cells and tissues.
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| Molecular Formula |
C5H10O5
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|---|---|
| Molecular Weight |
152.14222574234
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| Exact Mass |
152.065
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| CAS # |
478506-32-0
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| Related CAS # |
D-Ribose(mixture of isomers);50-69-1
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| Appearance |
White to off-white solid powder
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| LogP |
-2.5
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| InChi Key |
SRBFZHDQGSBBOR-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C5H10O5/c6-2-1-10-5(9)4(8)3(2)7/h2-9H,1H2
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| Chemical Name |
oxane-2,3,4,5-tetrol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~250 mg/mL (~1643.22 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.5729 mL | 32.8645 mL | 65.7289 mL | |
| 5 mM | 1.3146 mL | 6.5729 mL | 13.1458 mL | |
| 10 mM | 0.6573 mL | 3.2864 mL | 6.5729 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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