| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg | |||
| Other Sizes |
| Targets |
- Deoxycytidine triphosphate (dCTP) acts as a competitive substrate targeting DNA polymerase, interfering with the incorporation of cytarabine triphosphate (Ara-CTP) into DNA [1]
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|---|---|
| ln Vitro |
The study examined the sensitivity of 13 cell lines that were produced from mouse lymphoma, myeloma, mastocytoma, and 1-β-D-arabinofuranosylcytosine (ara-C) to growth inhibition. The results showed that the cell lines varied by a factor of 100 between the most and least susceptible. It was shown that sensitivity and cellular deoxycytidine triphosphate (2′-Deoxycytidine-5′-triphosphate, dCTP) concentration were inversely connected. This suggests that if cells decrease their dCTP content while exposed to ara-C, their sensitivity may also decline. Boost. 1]. Phosphoethanolamine cytidylyltransferase uses deoxycytidine triphosphate trisodium salt as a substrate [2].
- In cultured L1210 mouse leukemia cells, treatment with thymidine (TdR) increased intracellular dCTP levels, which concentration-dependently reduced the cytotoxicity of cytarabine (Ara-C). At a TdR concentration of 10 μM, intracellular dCTP levels were significantly elevated, and the killing effect of Ara-C on L1210 cells was attenuated by approximately 50%. Removal of TdR restored dCTP levels to normal, and the sensitivity to Ara-C was recovered accordingly [1] - In human cells (including normal peripheral blood lymphocytes and leukemia cells), dCTP is a key substrate for DNA synthesis, with its intracellular concentration maintained in dynamic equilibrium. dCTP levels were higher in certain leukemia cells than in normal lymphocytes. Exposure to chemotherapeutic drugs (e.g., antimetabolites) led to a significant decrease in dCTP levels, impairing DNA replication efficiency [3] |
| Cell Assay |
- Assay for L1210 cell sensitivity to Ara-C: L1210 mouse leukemia cells were seeded in culture plates and divided into control group, Ara-C alone group, and TdR (1-100 μM) pretreatment + Ara-C group. After 24-hour TdR pretreatment, different concentrations of Ara-C were added for further 48-hour culture. Cell survival was evaluated by cell counting or viability assay (e.g., trypan blue exclusion method) to analyze the effect of elevated dCTP levels on Ara-C cytotoxicity [1]
- Assay for deoxyribonucleoside triphosphates in human cells: Normal peripheral blood lymphocytes or leukemia cells were collected and lysed with ice-cold extraction buffer. The supernatant was obtained by centrifugation. Deoxyribonucleoside triphosphates in the extract, including dCTP, dATP, dGTP, and dTTP, were separated by high-performance liquid chromatography (HPLC). The intracellular concentration of each deoxyribonucleoside triphosphate was quantified based on retention time and peak area, comparing concentration differences between different cell types or after drug treatment [3] |
| References | |
| Additional Infomation |
DCTP is a 2'-deoxycytidine phosphate with cytosine as its nucleobase. It is a metabolite in humans, as well as in E. coli and mice. It is a 2'-deoxycytidine phosphate, and also a pyrimidine 2'-deoxyribonucleoside 5'-triphosphate. It is the conjugate acid of dCTP(3-). dCTP is a metabolite found or produced in E. coli (K12 strain, MG1655 strain). Deoxycytidine 5'-triphosphate is a metabolite found or produced in E. coli (K12 strain, MG1655 strain). 2'-Deoxycytidine-5'-triphosphate has been reported to exist in Homo sapiens, cattle, and Apis cerana, and there is relevant data. dCTP is a metabolite found or produced in Saccharomyces cerevisiae.
- Deoxycytidine triphosphate (dCTP) is a deoxynucleoside triphosphate substrate essential for DNA replication and repair, providing cytidine residues for DNA chain elongation [3] - In cytarabine (Ara-C) chemotherapeutic drugs, dCTP competes with Ara-CTP (the active metabolite of Ara-C) for DNA polymerase binding sites. Intracellular dCTP levels directly affect the efficiency of Ara-CTP incorporation into DNA, thereby regulating the antitumor activity of Ara-C [1]. The concentration of dCTP in human cells is jointly regulated by deoxynucleoside triphosphate synthase, kinase, and phosphatase. Abnormal dCTP levels (elevation or decrease) may affect cell proliferation, DNA damage repair, and sensitivity to chemotherapeutic drugs [3]. |
| Molecular Formula |
C9H16N3O13P3
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|---|---|
| Molecular Weight |
467.15704
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| Exact Mass |
466.989
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| CAS # |
2056-98-6
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| Related CAS # |
Deoxycytidine triphosphate trisodium salt;109909-44-6
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| PubChem CID |
65091
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| Appearance |
White to off-white solid powder
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| Density |
2.38 g/cm3
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| Boiling Point |
811.5ºC at 760 mmHg
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| Flash Point |
444.6ºC
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| Vapour Pressure |
1.73E-30mmHg at 25°C
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| Index of Refraction |
1.775
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| LogP |
-5.6
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| Hydrogen Bond Donor Count |
6
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| Hydrogen Bond Acceptor Count |
13
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| Rotatable Bond Count |
8
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| Heavy Atom Count |
28
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| Complexity |
823
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| Defined Atom Stereocenter Count |
3
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| SMILES |
C1[C@@H]([C@H](O[C@H]1N2C=CC(=NC2=O)N)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O
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| InChi Key |
RGWHQCVHVJXOKC-SHYZEUOFSA-N
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| InChi Code |
InChI=1S/C9H16N3O13P3/c10-7-1-2-12(9(14)11-7)8-3-5(13)6(23-8)4-22-27(18,19)25-28(20,21)24-26(15,16)17/h1-2,5-6,8,13H,3-4H2,(H,18,19)(H,20,21)(H2,10,11,14)(H2,15,16,17)/t5-,6+,8+/m0/s1
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| Chemical Name |
[[(2R,3S,5R)-5-(4-amino-2-oxopyrimidin-1-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~50 mg/mL (~107.03 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1406 mL | 10.7030 mL | 21.4059 mL | |
| 5 mM | 0.4281 mL | 2.1406 mL | 4.2812 mL | |
| 10 mM | 0.2141 mL | 1.0703 mL | 2.1406 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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