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dCTP

Alias: Cytidine 2deoxy 5(tetrahydrogen triphosphate) trisodium salt; Cytidine 5(tetrahydrogen triphosphate) 2deoxy trisodium salt; dCTP Thymus
Cat No.:V19298 Purity: ≥98%
Deoxycytidine triphosphate trisodium salt (dCTP trisodium salt) is a nucleoside triphosphate that may be utilized in DNA synthesis.
dCTP
dCTP Chemical Structure CAS No.: 109909-44-6
Product category: New1
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
5mg
10mg
50mg
100mg
250mg
500mg
Other Sizes

Other Forms of dCTP:

  • Deoxycytidine triphosphate (dCTP)
Official Supplier of:
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Top Publications Citing lnvivochem Products
Product Description
Deoxycytidine triphosphate trisodium salt (dCTP trisodium salt) is a nucleoside triphosphate that may be utilized in DNA synthesis. Deoxycytidine triphosphate trisodium salt may be utilized in real-time PCR, cDNA synthesis and DNA sequencing research.
Biological Activity I Assay Protocols (From Reference)
ln Vitro
The study conducted growth factor fingerprinting of 1-beta-D-Arabic Syriac glycosyl clock pin (ara-C) and found that it was present in 13 cell lines generated from murine myeloma, myeloid tumor-like, and mastocytoma. The outer 100-fold folds into each other between the most and least sensitive spectra. The amount of cellular deoxycytidine triphosphate (2'-Deoxycytidine-5'-triphosphate, dCTP) was discovered to be inversely linked with obesity[1], indicating that if cells are exposed to ara-C. Phosphoethanolamine glycol ester transferase uses deoxycytidine triphosphate trisodium salt as a substrate [2].
References

[1]. Effect of thymidine on the sensitivity of cultured mouse tumor cells to 1-beta-D-arabinofuranosylcytosine. Cancer Res. 1979 Feb;39(2 Pt 1):538-41.

[2]. CTP:phosphoethanolamine cytidylyltransferase. Biochim Biophys Acta. 1997 Sep 4;1348(1-2):91-9.

[3]. Deoxyribonucleoside triphosphates in human cells: changes in disease and following exposure to drugs. Eur J Clin Invest. 1975 Apr;5(2):191-202.

These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C9H16N3O13P3
Molecular Weight
467.15693
Exact Mass
532.935
CAS #
109909-44-6
Related CAS #
Deoxycytidine triphosphate;2056-98-6
PubChem CID
53486366
Appearance
Colorless to light yellow liquid
LogP
0.713
Hydrogen Bond Donor Count
3
Hydrogen Bond Acceptor Count
13
Rotatable Bond Count
8
Heavy Atom Count
31
Complexity
805
Defined Atom Stereocenter Count
3
SMILES
P(=O)([O-])(OP(=O)([O-])OP(=O)([O-])O[H])OC([H])([H])[C@]1([H])[C@]([H])(C([H])([H])[C@]([H])(N2C(N=C(C([H])=C2[H])N([H])[H])=O)O1)O[H].[Na+].[Na+].[Na+]
InChi Key
WBIPTAOOMJEGQO-MILVPLDLSA-K
InChi Code
InChI=1S/C9H16N3O13P3.3Na/c10-7-1-2-12(9(14)11-7)8-3-5(13)6(23-8)4-22-27(18,19)25-28(20,21)24-26(15,16)17;;;/h1-2,5-6,8,13H,3-4H2,(H,18,19)(H,20,21)(H2,10,11,14)(H2,15,16,17);;;/q;3*+1/p-3/t5-,6+,8+;;;/m0.../s1
Chemical Name
trisodium;[[[(2R,3S,5R)-5-(4-amino-2-oxopyrimidin-1-yl)-3-hydroxyoxolan-2-yl]methoxy-oxidophosphoryl]oxy-oxidophosphoryl] hydrogen phosphate
Synonyms
Cytidine 2deoxy 5(tetrahydrogen triphosphate) trisodium salt; Cytidine 5(tetrahydrogen triphosphate) 2deoxy trisodium salt; dCTP Thymus
HS Tariff Code
2934.99.9001
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
H2O : ~250 mg/mL (~468.96 mM)
DMSO :< 1 mg/mL
Solubility (In Vivo)
Solubility in Formulation 1: 100 mg/mL (187.58 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.

 (Please use freshly prepared in vivo formulations for optimal results.)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 2.1406 mL 10.7030 mL 21.4059 mL
5 mM 0.4281 mL 2.1406 mL 4.2812 mL
10 mM 0.2141 mL 1.0703 mL 2.1406 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

Calculator

Molarity Calculator allows you to calculate the mass, volume, and/or concentration required for a solution, as detailed below:

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An example of molarity calculation using the molarity calculator is shown below:
What is the mass of compound required to make a 10 mM stock solution in 5 ml of DMSO given that the molecular weight of the compound is 350.26 g/mol?
  • Enter 350.26 in the Molecular Weight (MW) box
  • Enter 10 in the Concentration box and choose the correct unit (mM)
  • Enter 5 in the Volume box and choose the correct unit (mL)
  • Click the “Calculate” button
  • The answer of 17.513 mg appears in the Mass box. In a similar way, you may calculate the volume and concentration.

Dilution Calculator allows you to calculate how to dilute a stock solution of known concentrations. For example, you may Enter C1, C2 & V2 to calculate V1, as detailed below:

What volume of a given 10 mM stock solution is required to make 25 ml of a 25 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=25 μM, V2=25 ml and V1 is the unknown:
  • Enter 10 into the Concentration (Start) box and choose the correct unit (mM)
  • Enter 25 into the Concentration (End) box and select the correct unit (mM)
  • Enter 25 into the Volume (End) box and choose the correct unit (mL)
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  • The answer of 62.5 μL (0.1 ml) appears in the Volume (Start) box
g/mol

Molecular Weight Calculator allows you to calculate the molar mass and elemental composition of a compound, as detailed below:

Note: Chemical formula is case sensitive: C12H18N3O4  c12h18n3o4
Instructions to calculate molar mass (molecular weight) of a chemical compound:
  • To calculate molar mass of a chemical compound, please enter the chemical/molecular formula and click the “Calculate’ button.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
  • Molecular mass (or molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
  • Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
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Reconstitution Calculator allows you to calculate the volume of solvent required to reconstitute your vial.

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  • The answer appears in the Volume (to add to vial) box
In vivo Formulation Calculator (Clear solution)
Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)
Step 2: Enter in vivo formulation (This is only a calculator, not the exact formulation for a specific product. Please contact us first if there is no in vivo formulation in the solubility section.)
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Calculation results

Working concentration mg/mL;

Method for preparing DMSO stock solution mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
             (2) Be sure to add the solvent(s) in order.

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