| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| Targets |
DNA polymerases. dCTP serves as a substrate for DNA polymerases during DNA synthesis, providing the deoxycytidine moiety required for incorporation into growing DNA strands. It pairs with deoxyguanosine (dG) in the template strand through complementary base pairing. dCTP is also a substrate for other enzymes involved in nucleic acid metabolism.
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| ln Vitro |
The study conducted growth factor fingerprinting of 1-beta-D-Arabic Syriac glycosyl clock pin (ara-C) and found that it was present in 13 cell lines generated from murine myeloma, myeloid tumor-like, and mastocytoma. The outer 100-fold folds into each other between the most and least sensitive spectra. The amount of cellular deoxycytidine triphosphate (2'-Deoxycytidine-5'-triphosphate, dCTP) was discovered to be inversely linked with obesity[1], indicating that if cells are exposed to ara-C. Phosphoethanolamine glycol ester transferase uses deoxycytidine triphosphate trisodium salt as a substrate [2].
dCTP is a standard component in PCR reaction mixtures, providing the cytidine nucleotides required for DNA amplification. In vitro, dCTP is incorporated into newly synthesized DNA by DNA polymerases during primer extension. It is essential for the synthesis of DNA in various molecular biology applications including real-time PCR, cDNA synthesis, and DNA sequencing. |
| ln Vivo |
dCTP is used in vivo as a substrate for DNA synthesis during cell division and DNA repair. It is an essential nucleotide for DNA replication in all living cells. In research applications, dCTP is used in various molecular biology techniques. The compound is available as a 100 mM solution for convenient use in laboratory settings.
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| Enzyme Assay |
In vitro enzyme assays using dCTP typically involve DNA polymerase activity assays. The incorporation of dCTP into DNA can be monitored using radiolabeled dCTP (e.g., [α-32P]dCTP) or fluorescently labeled dCTP. DNA polymerases are incubated with a DNA template, primer, and dCTP along with other dNTPs (dATP, dGTP, dTTP). Polymerase activity is quantified by measuring incorporated radioactivity or fluorescence.
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| Cell Assay |
In vitro cellular studies with dCTP are not typically performed because dCTP is a natural nucleotide that is taken up and metabolized by cells. However, cell-based assays may involve the use of dCTP analogs or modified dCTP derivatives to study DNA synthesis, nucleotide metabolism, or the effects of nucleotide analogs on cell proliferation. dCTP is commonly used in cell-free systems for DNA synthesis reactions.
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| Animal Protocol |
dCTP is not administered to animals as a therapeutic agent but is used as a reagent in molecular biology research. In vivo, dCTP is a natural metabolite that is synthesized de novo or via salvage pathways. Exogenously administered dCTP would be rapidly metabolized by phosphatases and other enzymes. Animal studies using dCTP are not typical for this compound.
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| ADME/Pharmacokinetics |
dCTP trisodium salt has a molecular weight of 533.10 and molecular formula C9H13N3Na3O13P3. It is supplied as a colorless to light yellow liquid solution, typically at 100 mM concentration. The compound is stable when stored at -20°C. dCTP is highly soluble in water and aqueous buffers. Purity is typically ≥98% or higher.
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| Toxicity/Toxicokinetics |
dCTP is a naturally occurring nucleotide with low toxicity. It is an essential cellular metabolite and is generally recognized as safe for use in molecular biology applications. When used as a reagent, standard laboratory safety precautions should be followed. No significant toxicity is associated with the use of dCTP in research settings at the concentrations typically employed.
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| References | |
| Additional Infomation |
dCTP is a 2'-deoxycytidine phosphate with cytosine as its nucleobase. It is a metabolite found in mice, *E. coli*, and humans. It is a pyrimidine 2'-deoxyribonucleoside 5'-triphosphate and 2'-deoxycytidine phosphate. It is the conjugate acid of dCTP(3-). dCTP is a metabolite found in or produced by *E. coli* (K12 strain, MG1655 strain). Deoxycytidine 5'-triphosphate is a metabolite found in or produced by *E. coli* (K12 strain, MG1655 strain). 2'-Deoxycytidine-5'-triphosphate has been reported in *Homo sapiens*, cattle, and *Apis cerana*, but the relevant data are unclear. dCTP is a metabolite found or produced in *Saccharomyces cerevisiae*. The RN given here refers to the unlabeled parent compound. See other relationships…
dCTP (2'-deoxycytidine-5'-O-triphosphate) is a nucleoside triphosphate that serves as one of the four building blocks of DNA, along with dATP, dGTP, and dTTP. It is essential for DNA synthesis and repair, and is widely used in molecular biology research. dCTP is a substrate for DNA polymerases in PCR, real-time PCR, cDNA synthesis, and DNA sequencing applications. The compound is not a drug and has no therapeutic indications; it is a research reagent used in biochemistry and molecular biology laboratories. |
| Molecular Formula |
C9H16N3O13P3
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|---|---|
| Molecular Weight |
467.15693
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| Exact Mass |
532.935
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| CAS # |
109909-44-6
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| Related CAS # |
Deoxycytidine triphosphate;2056-98-6
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| PubChem CID |
53486366
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| Appearance |
Colorless to light yellow liquid
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| LogP |
0.713
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
13
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| Rotatable Bond Count |
8
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| Heavy Atom Count |
31
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| Complexity |
805
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| Defined Atom Stereocenter Count |
3
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| SMILES |
P(=O)([O-])(OP(=O)([O-])OP(=O)([O-])O[H])OC([H])([H])[C@]1([H])[C@]([H])(C([H])([H])[C@]([H])(N2C(N=C(C([H])=C2[H])N([H])[H])=O)O1)O[H].[Na+].[Na+].[Na+]
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| InChi Key |
WBIPTAOOMJEGQO-MILVPLDLSA-K
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| InChi Code |
InChI=1S/C9H16N3O13P3.3Na/c10-7-1-2-12(9(14)11-7)8-3-5(13)6(23-8)4-22-27(18,19)25-28(20,21)24-26(15,16)17;;;/h1-2,5-6,8,13H,3-4H2,(H,18,19)(H,20,21)(H2,10,11,14)(H2,15,16,17);;;/q;3*+1/p-3/t5-,6+,8+;;;/m0.../s1
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| Chemical Name |
trisodium;[[[(2R,3S,5R)-5-(4-amino-2-oxopyrimidin-1-yl)-3-hydroxyoxolan-2-yl]methoxy-oxidophosphoryl]oxy-oxidophosphoryl] hydrogen phosphate
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| Synonyms |
Cytidine 2deoxy 5(tetrahydrogen triphosphate) trisodium salt; Cytidine 5(tetrahydrogen triphosphate) 2deoxy trisodium salt; dCTP Thymus
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~250 mg/mL (~468.96 mM)
DMSO :< 1 mg/mL |
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| Solubility (In Vivo) |
Solubility in Formulation 1: 100 mg/mL (187.58 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.
(Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1406 mL | 10.7030 mL | 21.4059 mL | |
| 5 mM | 0.4281 mL | 2.1406 mL | 4.2812 mL | |
| 10 mM | 0.2141 mL | 1.0703 mL | 2.1406 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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