| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| Other Sizes |
| Targets |
Coenzyme Q10 transfers electrons from complexes I and II to complex III in the mitochondrial electron transport chain and exists in oxidized (ubiquinone) and reduced (ubiquinol) forms; it also scavenges lipid peroxyl radicals and regenerates α-tocopherol, thereby protecting membrane lipids from oxidative damage, and it is a cofactor for uncoupling proteins and dihydroorotate dehydrogenase.
|
|---|---|
| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
In vitro, coenzyme Q10-d6 is taken up by cultured hepatocytes, cardiomyocytes, and fibroblasts and incorporated into mitochondrial membranes, where it supports electron transport and ATP production; it inhibits DMN-induced liver fibrosis in H4IIE hepatoma cells through NRF2/ARE activation, and its protective effects are abolished in Nrf2-null cells, indicating NRF2-dependent antioxidant signaling. |
| ln Vivo |
In vivo, dietary coenzyme Q10-d6 is absorbed and distributed to liver, heart, skeletal muscle, and brain, where it enriches mitochondrial and plasma membrane fractions; CoQ10 supplementation improves cardiac function and reduces oxidative stress markers in aging rodents and in models of heart failure, and it attenuates liver fibrosis and renal injury in chronic disease models through antioxidant and mitochondrial-protective mechanisms.
|
| Enzyme Assay |
Noncellular assays quantify coenzyme Q10-d6 and its reduced form ubiquinol-d6 in plasma, tissue homogenates, and mitochondrial fractions by liquid chromatography–tandem mass spectrometry after lipid extraction; mitochondrial respiratory chain activity is measured in isolated submitochondrial particles or permeabilized cells by monitoring NADH or succinate oxidation and cytochrome c reduction, and antioxidant capacity is assessed by lipid peroxidation assays.
|
| Cell Assay |
Cellular assays use cardiomyocytes, hepatocytes, or fibroblasts cultured with coenzyme Q10-d6–loaded lipoproteins or nanoparticles, followed by mitochondrial membrane potential measurement by tetramethylrhodamine fluorescence, ATP content by luciferase assay, and reactive oxygen species by dichlorofluorescein or MitoSOX; NRF2 nuclear translocation is assessed by immunoblotting.
|
| Animal Protocol |
Animal protocols administer coenzyme Q10-d6 orally in diet or by gavage to mice or rats at doses of 10–200 mg/kg/day for 1–12 weeks; tissues are collected for mass spectrometric quantification of CoQ10-d6 and ubiquinol-d6, and cardiac function by echocardiography, mitochondrial respiration by Seahorse analysis, and oxidative stress markers are evaluated to relate tissue CoQ10 enrichment to functional outcomes.
|
| References |
|
| Molecular Formula |
C59H90O4
|
|---|---|
| Molecular Weight |
863.343518733978
|
| Exact Mass |
868.721
|
| CAS # |
110971-02-3
|
| Related CAS # |
Coenzyme Q10;303-98-0
|
| PubChem CID |
10854936
|
| Appearance |
Yellow to orange solid
|
| Density |
1.0±0.1 g/cm3
|
| Boiling Point |
869.0±65.0 °C at 760 mmHg
|
| Flash Point |
324.6±34.3 °C
|
| Vapour Pressure |
0.0±3.3 mmHg at 25°C
|
| Index of Refraction |
1.526
|
| LogP |
20.93
|
| tPSA |
52.6
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
31
|
| Heavy Atom Count |
63
|
| Complexity |
1840
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
C(C1=C(C(=O)C(OC([H])([H])[H])=C(OC([H])([H])[H])C1=O)C)/C=C(\C)/CC/C=C(\C)/CC/C=C(\C)/CC/C=C(\C)/CC/C=C(\C)/CC/C=C(\C)/CC/C=C(\C)/CC/C=C(\C)/CC/C=C(\C)/CCC=C(C)C
|
| InChi Key |
ACTIUHUUMQJHFO-YDQRCUKRSA-N
|
| InChi Code |
InChI=1S/C59H90O4/c1-44(2)24-15-25-45(3)26-16-27-46(4)28-17-29-47(5)30-18-31-48(6)32-19-33-49(7)34-20-35-50(8)36-21-37-51(9)38-22-39-52(10)40-23-41-53(11)42-43-55-54(12)56(60)58(62-13)59(63-14)57(55)61/h24,26,28,30,32,34,36,38,40,42H,15-23,25,27,29,31,33,35,37,39,41,43H2,1-14H3/b45-26+,46-28+,47-30+,48-32+,49-34+,50-36+,51-38+,52-40+,53-42+/i13D3,14D3
|
| Chemical Name |
2-[(2E,6E,10E,14E,18E,22E,26E,30E,34E)-3,7,11,15,19,23,27,31,35,39-decamethyltetraconta-2,6,10,14,18,22,26,30,34,38-decaenyl]-3-methyl-5,6-bis(trideuteriomethoxy)cyclohexa-2,5-diene-1,4-dione
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.1583 mL | 5.7915 mL | 11.5829 mL | |
| 5 mM | 0.2317 mL | 1.1583 mL | 2.3166 mL | |
| 10 mM | 0.1158 mL | 0.5791 mL | 1.1583 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
