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    Chrysophanic Acid (Chrysophanol)
    Chrysophanic Acid (Chrysophanol)

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    This product is for research use only, not for human use. We do not sell to patients.
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    InvivoChem Cat #: V0207
    CAS #: 481-74-3 Purity ≥98%

    Description: Chrysophanic acid (also called Chrysophanol), a naturally occuring anthraquinone isolated from Dianella longifolia, is a selective and potent inhibitor of EGFR/mTOR (epidermal growth factor (EGF) receptor/mammalian target of rapamycin) with potential anti-obesity and antitumor activity. Chrysophanol (CA) improves high-fat diet (HFD)-induced obesity in C57BL/6 Mice. It also showed potent antiproliferative and ancticancer activity in EGFR-overexpressing SNU-C5 human colon cancer cells. It preferentially blocked proliferation of SNU-C5 cells but not in other cell lines with low levels of EGFR expression.

    References: Phytother Res. 2011 Jun;25(6):833-7; Antiviral Res. 2001 Mar;49(3):169-78.   

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    Molecular Weight (MW)254.24
    FormulaC15H10O4
    CAS No.481-74-3
    Storage-20℃ for 3 years in powder form
    -80℃ for 2 years in solvent
    Solubility (In vitro)DMSO: 5 mg/mL (19.66 mM)
    Water: <1 mg/mL
    Ethanol: <1 mg/mL
    Other info

    Chemical Name: 1,8-dihydroxy-3-methylanthracene-9,10-dione

    InChi Key: 1,8-dihydroxy-3-methylanthracene-9,10-dione

    InChi Code: InChI=1S/C15H10O4/c1-7-5-9-13(11(17)6-7)15(19)12-8(14(9)18)3-2-4-10(12)16/h2-6,16-17H,1H3

    SMILES Code: O=C1C2=C(C=CC=C2O)C(C3=CC(C)=CC(O)=C13)=O

    Synonyms Chrysophanic acid; Chrysophanol


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    n Vitro

    In Vitro activity: Chrysophanic acid (Chrysophanol) is a EGFR/mTOR pathway inhibitor. Chrysophanic acid (Chrysophanol) is a natural anthraquinone, has anticancer activity in EGFR-overexpressing SNU-C5 human colon cancer cells. Chrysophanic acid (Chrysophanol) preferentially blocks proliferation in SNU-C5 cells but not in other cell lines (HT7, HT29, KM12C, SW480, HCT116 and SNU-C4) with low levels of EGFR expression. Chrysophanic acid (Chrysophanol) treatment in SNU-C5 cells inhibits EGF-induced phosphorylation of EGFR and suppresses activation of downstream signaling molecules, such as AKT, extracellular signal-regulated kinase (ERK) and the mammalian target of rapamycin (mTOR)/ribosomal protein S6 kinase (p70S6K). Chrysophanic acid also inhibits the replication of poliovirus types 2 and 3 (Picornaviridae) and poliovirus-induced cytopathic effects in BGM (Buffalo green monkey) kidney cells.


    Cell Assay: The cells are seeded at 5×103 cells/mL in 96-well microplates and allowed to attach for 24 h. Chrysophanol (20, 50, 80 and 120 μM) is added to the medium at various concentrations up to 120 μM and for different durations. After treatment, cell cytotoxicity and/or proliferation is assessed by a Cell Counting Kit-8 (CCK-8). Briefly, highly water-soluble tetrazolium salt, WST-8, produces an orange colored water-soluble product, formazan. The amount of formazan dye generated by dehydrogenases in cells is directly proportional to the number of living cells.CCK-8 (10 μL) is added to each well and incubated for 3 h at 37°C, then cell proliferation and cytotoxicity are assessed by measuring the absorbance at 450 nm using a microplate reader.Three replicated wells are used for each experimental condition

    In VivoChrysophanol (CA) improves HFD-induced obesity in C57BL/6 Mice. The in vivo performance of Chrysophanol is performed in male C57BL/6J mice to determine the efficacy of administered Chrysophanol. Mice fed the HFD gained significantly more weight than those fed the standard diet mice. On the other hand, weight gain of Chrysophanol group is significantly less than with the untreated HFD. Mice in the HFD-group gained 23.92 ± 1.74 g of weight, while those in the Chrysophanol group gained 16.72±2 g of weight after 16 weeks
    Animal modelMice: Male 4-week-old C57BL/6J mice are maintained for 1 week prior to experiments. Mice are maintained on a 12-h light/dark cycle in a pathogen-free animal facility, provided with laboratory diet and water ad libitum. To induce obesity, the mice are fed a HFD with 60% kcal% fat. Control group (C) are fed a commercial standard chow diet. HFD group (HFD) mice are fed with HFD only. HFD plus CA group (CA) Mice are fed with HFD for 4 weeks before administration of Chrysophanol (5 mg/kg/day). The mice are divided into three groups (n = 5) that are fed chow diet, HFD, and HFD plus Chrysophanol for 16 weeks. Body weight and food intake are measured three times per week.
    Formulation & Dosage5 mg/kg
    References

    Phytother Res. 2011 Jun;25(6):833-7; Antiviral Res. 2001 Mar;49(3):169-78.


    These protocols are for reference only. InvivoChem does not independently validate these methods.

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