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    BMH-21
    BMH-21

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    This product is for research use only, not for human use. We do not sell to patients.
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    InvivoChem Cat #: V1435
    CAS #: 896705-16-1Purity ≥98%

    Description: BMH-21 (BMH 21; BMH21) is a novel and potent DNA intercalator with potential antineoplastic activity. It acts by binding to ribosomal DNA and inhibiting RNA polymerase I (Pol I) transcription. BMH-21 intercalated into dsDNA and had binding preference towards GC-rich DNA sequences. BMH-21 had wide cytotoxic activities against human cancer cell lines, and acted in p53-independent manner, widely considered as a mediator of many cytotoxic agents. BMH-21 inhibited transcription of RNA polymerase I (Pol I) by binding to ribosomal (r) DNA that caused Pol I blockade and degradation of the large catalytic subunit of Pol I, RPA194. 

    References: Cancer Cell. 2014 Jan 13;25(1):77-90; J Med Chem. 2014 Jun 12;57(11):4950-61. 


    Publications Citing Use of InvivoChem BMH-21Cell Stem Cell. 2020 Jun 4;26(6):845-861.e12

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    Molecular Weight (MW)360.41 
    FormulaC21H20N4O2 
    CAS No.896705-16-1 
    Storage-20℃ for 3 years in powder form
    -80℃ for 2 years in solvent
    Solubility (In vitro)DMSO: 5 mg/mL (13.9 mM) 
    Water: <1 mg/mL
    Ethanol: 2 mg/mL (5.5 mM) 
    Other Info

    Exact Mass: 362.1743 

    Chemical Name: N-(2-(dimethylamino)ethyl)-12-oxo-11a,12-dihydro-5aH-benzo[g]pyrido[2,1-b]quinazoline-4-carboxamide

    SMILES Code: O=C(C1=CC=CN2C1=NC3C=C(C=CC=C4)C4=CC3C2=O)NCCN(C)C

    SynonymsBMH 21; BMH-21; BMH21; 


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    In Vitro

    In vitro activity: BMH-21 causes proteasome-dependent destruction of RPA194, the large catalytic subunit protein of Pol I holocomplex. In U2OS cancer cell line, BMH-21 results in degradation of RPA194 and translocation of NCL with IC50 of 0.05 μM and 0.07 μM, respectively. By causing nucleolar stress, BMH-21 also shows potent inhibition on cell viability.


    Cell Assay: The cells (U2OS osteosarcoma cells) are maintained at 37 °C in a humidified atmosphere containing 5% CO2. U2OS osteosarcoma cells are cultured in DMEM supplemented with 15% fetal bovine serum. Cells are plated in 96-well plates at a density of 10000 cells/well in triplicate and incubated for 48 h with the compounds. Viability is determined using WST-1 cell proliferation reagent.

    In VivoBMH-21 reduces tumor growth in mouse xenografts. 
    Animal modelMouse
    Formulation & Dosage25 mg/kg; 50 mg/kg; injection
    ReferencesCancer Cell. 2014 Jan 13;25(1):77-90; J Med Chem. 2014 Jun 12;57(11):4950-61.  


    These protocols are for reference only. InvivoChem does not independently validate these methods.

    BMH-21

    Cancer Cell. 2014 Jan 13;25(1):77-90.
     

    BMH-21



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