BAPTA Tetrasodium

Alias: BAPTA-Na4 BAPTA Na4 BAPTANa4 BAPTA Tetrasodium
Cat No.:V6123 Purity: ≥98%
BAPTA tetrasodium is a selective calcium chelator.
BAPTA Tetrasodium Chemical Structure CAS No.: 126824-24-6
Product category: Phospholipase
This product is for research use only, not for human use. We do not sell to patients.
Size Price Stock Qty
100mg
250mg
500mg
1g
2g
5g
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Other Forms of BAPTA Tetrasodium:

  • BAPTA
  • BAPTA tetrapotassium
Official Supplier of:
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Purity & Quality Control Documentation

Purity: ≥98%

Product Description
BAPTA tetrasodium is a selective calcium chelator. BAPTA tetrasodium, as a calcium ion indicator, has high selectivity for magnesium ions and calcium ions. BAPTA tetrasodium is extensively used as an intracellular buffer to study the effects of Ca2+ release from intracellular stores or influx through plasma membrane Ca2+ permeable/penetrable channels. BAPTA tetrasodium also inhibits the activity of phospholipase C, independent of its calcium chelating properties.
Biological Activity I Assay Protocols (From Reference)
ln Vitro
BAPTA (0.3-30 μM; 1 h) inhibits calcium signaling in monodifferentiated NH15-CA2 neuroblastoma and glioma hybrid cells, although it can be employed to stop [Ca2+]-induced cell damage [3]. Phospholipase C (PLC) activity is inhibited by BAPTA (0–10 mM) in a dose-dependent and Ca2+-independent manner [2].
References
[1]. R Y Tsien, et al. New calcium indicators and buffers with high selectivity against magnesium and protons: design, synthesis, and properties of prototype structures. Biochemistry. 1980 May 27;19(11):2396-404.
[2]. Roger C Hardie, et al. Inhibition of phospholipase C activity in Drosophila photoreceptors by 1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid (BAPTA) and di-bromo BAPTA. Cell Calcium. 2005 Dec;38(6):547-56.
[3]. M B Collatz, et al. Intracellular calcium chelator BAPTA protects cells against toxic calcium overload but also alters physiological calcium responses. Cell Calcium
These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C22H20N2NA4O10
Molecular Weight
564.37
CAS #
126824-24-6
SMILES
O=C(O[Na])CN(CC(O[Na])=O)C1=CC=CC=C1OCCOC2=CC=CC=C2N(CC(O[Na])=O)CC(O[Na])=O
InChi Key
ZWSMLJACYSGFKD-UHFFFAOYSA-J
InChi Code
InChI=1S/C22H24N2O10.4Na/c25-19(26)11-23(12-20(27)28)15-5-1-3-7-17(15)33-9-10-34-18-8-4-2-6-16(18)24(13-21(29)30)14-22(31)32/h1-8H,9-14H2,(H,25,26)(H,27,28)(H,29,30)(H,31,32)/q4*+1/p-4
Chemical Name
sodium 2,2',2'',2'''-(((ethane-1,2-diylbis(oxy))bis(2,1-phenylene))bis(azanetriyl))tetraacetate
Synonyms
BAPTA-Na4 BAPTA Na4 BAPTANa4 BAPTA Tetrasodium
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month

Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
H2O : ~100 mg/mL (~177.19 mM)
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 1.7719 mL 8.8594 mL 17.7189 mL
5 mM 0.3544 mL 1.7719 mL 3.5438 mL
10 mM 0.1772 mL 0.8859 mL 1.7719 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

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