| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| 250mg |
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| 500mg |
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| 1g |
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| Other Sizes |
| Targets |
APS-5 targets alkaline phosphatase (AP) as a substrate. It is a chemiluminescent substrate for AP, which hydrolyzes the phosphate group, leading to a chemiluminescent reaction. The compound is used for diagnostic immune detection, such as tumor markers, infectious diseases, endocrine function, and hormones.
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| ln Vitro |
Advice (This is our suggested protocol, which should be adjusted to suit your particular circumstances as it simply offers guidance). Activity Assay for Alkaline Phosphatase[1]: 1. Combine the test sample (10 mg) with 100 μL of Lumigen APS-5 and spin at maximum speed for 10 seconds. Ten additional μL of water should be added to the mixture. 2. Non-bacterial ALP can be rendered inactive by heating the combined sample for one minute in a water bath at 75°C. After heating, transfer it to a 96-well plate and start measuring right away. Utilizing a photometer, ascertain ALP activity.
In vitro, APS-5 is a chemiluminescent substrate for alkaline phosphatase. When mixed with alkaline phosphatase, the phosphate group is hydrolyzed, resulting in continuous, stable, and efficient light emission. The compound is very suitable for phosphatase activity solution assays and phosphatase enzyme-linked immunoassays. |
| ln Vivo |
In vivo activity data for APS-5 are not applicable, as it is a diagnostic reagent used in vitro. It is not a therapeutic agent.
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| Enzyme Assay |
The in vitro assay for APS-5 involves mixing the substrate with alkaline phosphatase-conjugated antibodies in an ELISA format. The chemiluminescent signal is measured using a luminometer. The signal intensity is proportional to the amount of alkaline phosphatase present.
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| Cell Assay |
Cellular assays for APS-5 are not typically performed, as it is a diagnostic reagent used in ELISA applications.
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| Animal Protocol |
In vivo animal studies for APS-5 are not applicable, as it is a diagnostic reagent used in vitro.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for APS-5 are not applicable, as it is a diagnostic reagent used in vitro. The compound should be stored at room temperature under inert gas.
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| Toxicity/Toxicokinetics |
Toxicity data for APS-5 are not reported in the available sources. As a diagnostic reagent, it is handled in laboratory settings with standard safety precautions.
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| References | |
| Additional Infomation |
APS-5 is a chemiluminescent substrate for alkaline phosphatase used in ELISA applications. It is used for diagnostic immune detection. The compound is available from commercial suppliers for research and diagnostic purposes.
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| Molecular Formula |
C21H15CLNNA2O4PS
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|---|---|
| Molecular Weight |
489.819405794144
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| Exact Mass |
488.994
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| CAS # |
193884-53-6
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| PubChem CID |
9826873
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
31
|
| Complexity |
626
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CN1C2=CC=CC=C2C(=C(OP(=O)([O-])[O-])SC3=CC=C(C=C3)Cl)C4=CC=CC=C41.[Na+].[Na+]
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| InChi Key |
NDPRDNBCONYNMF-UHFFFAOYSA-L
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| InChi Code |
InChI=1S/C21H17ClNO4PS.2Na/c1-23-18-8-4-2-6-16(18)20(17-7-3-5-9-19(17)23)21(27-28(24,25)26)29-15-12-10-14(22)11-13-15;;/h2-13H,1H3,(H2,24,25,26);;/q;2*+1/p-2
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| Chemical Name |
disodium;[(4-chlorophenyl)sulfanyl-(10-methylacridin-9-ylidene)methyl] phosphate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.0416 mL | 10.2078 mL | 20.4157 mL | |
| 5 mM | 0.4083 mL | 2.0416 mL | 4.0831 mL | |
| 10 mM | 0.2042 mL | 1.0208 mL | 2.0416 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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