| Size | Price | Stock | Qty |
|---|---|---|---|
| 25mg |
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| 50mg |
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| Other Sizes |
| Targets |
The primary target of prochloraz manganese is the biosynthesis of ergosterol, a critical component of fungal cell membranes. By inhibiting specific enzymes involved in ergosterol synthesis, the compound disrupts cell membrane integrity, leading to fungal cell death. The manganese complex enhances stability while enabling dissociation in aqueous environments to release biologically active components.
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|---|---|
| ln Vitro |
In vitro studies have demonstrated the broad-spectrum antifungal activity of prochloraz manganese. The compound is effective against various fungal diseases that can harm crops such as wheat, barley, and fruits like apples and pears. Prochloraz manganese works by inhibiting the growth of fungal cells and preventing their spread. The compound's efficacy has been characterized in standard antifungal assays.
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| ln Vivo |
In vivo studies have confirmed the efficacy of prochloraz manganese in agricultural applications. The compound has protective and curative effects against a variety of fungal pathogens. It is effective against diseases caused by ascomycetes and other fungi. Prochloraz manganese is used in the agricultural industry to protect crops from fungal infections.
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| Enzyme Assay |
For antifungal activity screening, standard agar dilution or broth microdilution methods are used. Fungal pathogens are cultured on appropriate media and exposed to serial dilutions of prochloraz manganese. Mycelial growth inhibition is measured, and EC₅₀ values are calculated. Standard protocols for fungicide activity testing are described in the literature.
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| Cell Assay |
For in vitro cell-based studies, fungal cells are cultured in appropriate media and treated with serial dilutions of prochloraz manganese. Fungal growth is assessed by measuring colony diameter or optical density. The compound's effects on fungal cell membrane integrity and ergosterol content may be measured using biochemical assays.
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| Animal Protocol |
In vivo studies for prochloraz manganese are conducted in greenhouse or field settings. Crops are treated with the compound, and disease severity is assessed. The compound's protective and curative effects are evaluated. Standard protocols for fungicide efficacy testing in plants are followed.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of prochloraz manganese have not been extensively characterized for mammalian systems as the compound is an agricultural fungicide. The complex dissociates in aqueous environments to release prochloraz. Specific mammalian PK parameters have not been reported. The compound's environmental fate has been studied for agricultural use.
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| Toxicity/Toxicokinetics |
Toxicological data for prochloraz manganese are available from regulatory submissions. Prochloraz is a low-toxicity imidazole fungicide. The manganese complex may have a different toxicity profile compared to prochloraz alone. Standard toxicology studies have been conducted for agricultural registration.
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| References |
[1]. Jiang J, et al. Identification and degradation characteristics of Bacillus cereus strain WD-2 isolated from prochloraz-manganese-contaminated soils. PLoS One. 2019 Aug 9;14(8):e0220975.
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| Additional Infomation |
Prochloraz manganese is a commercial fungicide used in the agricultural industry. It is a coordination complex of prochloraz and manganese that enhances stability and efficacy. The compound is effective against various fungal pathogens. No clinical or therapeutic applications have been reported. Prochloraz manganese is available as a research compound for agricultural and analytical applications.
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| Molecular Formula |
C60H64CL14MNN12O8
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|---|---|
| Molecular Weight |
1632.51
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| Exact Mass |
1625
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| CAS # |
75747-77-2
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| PubChem CID |
71306902
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| Appearance |
Typically exists as solid at room temperature
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| LogP |
19.788
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
12
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| Rotatable Bond Count |
24
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| Heavy Atom Count |
95
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| Complexity |
380
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[Mn+2].CCCN(C(N1C=CN=C1)=O)CCOC1=C(Cl)C=C(Cl)C=C1Cl
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| InChi Key |
OXOKTPUZOHPXSA-UHFFFAOYSA-L
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| InChi Code |
InChI=1S/4C15H16Cl3N3O2.2ClH.Mn/c4*1-2-4-20(15(22)21-5-3-19-10-21)6-7-23-14-12(17)8-11(16)9-13(14)18;;;/h4*3,5,8-10H,2,4,6-7H2,1H3;2*1H;/q;;;;;;+2/p-2
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| Chemical Name |
dichloromanganese;N-propyl-N-[2-(2,4,6-trichlorophenoxy)ethyl]imidazole-1-carboxamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.6126 mL | 3.0628 mL | 6.1255 mL | |
| 5 mM | 0.1225 mL | 0.6126 mL | 1.2251 mL | |
| 10 mM | 0.0613 mL | 0.3063 mL | 0.6126 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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