| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| Other Sizes |
| Targets |
Palmitic acid-1-13C does not have a specific biological target itself but serves as a stable isotope tracer for studying lipid metabolism. As a 13C-labeled fatty acid, it can be used to track the metabolic fate of palmitic acid in biological systems. This allows researchers to study fatty acid synthesis, degradation, and incorporation into complex lipids. It is crucial in pharmaceutical research, nutrition science, and metabolic disease studies.
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|---|---|
| ln Vitro |
The in vitro activity of Palmitic acid-1-13C is assessed by its utility as an internal standard and tracer. The compound's isotopic purity and its behavior in analytical methods such as GC-MS or LC-MS are key measures of its utility. It is used to precisely quantify and track lipid metabolism and synthesis in biological systems.
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| ln Vivo |
In vivo studies for Palmitic acid-1-13C are conducted in animal models to study lipid metabolism. The compound is administered via oral gavage or intravenous injection, and its incorporation into various lipid pools is tracked over time by mass spectrometry. These studies provide insights into fatty acid processing and regulation in health and disease.
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| Enzyme Assay |
For analytical applications, Palmitic acid-1-13C is used as an internal standard in GC-MS or LC-MS methods. A known amount of the labeled compound is added to biological samples, and the ratio of labeled to unlabeled palmitic acid is measured. This allows for the precise quantification of palmitic acid in the sample.
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| Cell Assay |
For cellular studies, cells are cultured in media containing Palmitic acid-1-13C. The incorporation of the labeled fatty acid into cellular lipids is analyzed by mass spectrometry. This allows researchers to study fatty acid uptake, metabolism, and incorporation into complex lipids such as triglycerides and phospholipids.
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| Animal Protocol |
For in vivo metabolic studies, animals are administered Palmitic acid-1-13C via oral gavage or intravenous injection. Blood, urine, and tissue samples are collected at various time points. The 13C enrichment in palmitic acid and its metabolites is measured by mass spectrometry to study fatty acid metabolism, oxidation, and incorporation into tissues.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Palmitic acid-1-13C are similar to those of unlabeled palmitic acid. It is a solid with a melting point of 61-64°C. The compound is stable and should be stored under recommended conditions. As a stable isotope-labeled compound, it is not radioactive and poses minimal health risks when handled properly.
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| Toxicity/Toxicokinetics |
Palmitic acid-1-13C is generally considered safe for research use at appropriate concentrations. As a stable isotope-labeled compound, it is not radioactive and poses minimal health risks when handled properly. Standard laboratory safety precautions should be followed when handling this compound. It is intended for research use only and is not for human therapeutic applications.
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| References | |
| Additional Infomation |
Palmitic acid-1-13C is a stable isotope-labeled analog of palmitic acid with a molecular formula of 13CC15H32O2 and a molecular weight of 257.42. It is used as an internal standard for the quantification of palmitic acid by GC-MS or LC-MS. This research compound is for laboratory use only and has not been approved for clinical applications.
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| Molecular Formula |
C1513CH32O2
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|---|---|
| Molecular Weight |
257.42
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| Exact Mass |
257.244
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| CAS # |
57677-53-9
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| Related CAS # |
Palmitic acid;57-10-3;Palmitic acid sodium;408-35-5;Palmitic acid-1,2,3,4-13C4;287100-89-4
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| PubChem CID |
9881577
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| Appearance |
White to off-white solid powder
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| Density |
0.855 g/mL at 25ºC
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| Boiling Point |
271.5ºC100 mm Hg(lit.)
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| Melting Point |
61-64ºC(lit.)
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| Index of Refraction |
1.454
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| LogP |
5.552
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
14
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| Heavy Atom Count |
18
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| Complexity |
178
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CCCCCCCCCCCCCCC[13C](=O)O
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| InChi Key |
IPCSVZSSVZVIGE-LOYIAQTISA-N
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| InChi Code |
InChI=1S/C16H32O2/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16(17)18/h2-15H2,1H3,(H,17,18)/i16+1
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| Chemical Name |
(113C)hexadecanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : 66.67 mg/mL (258.99 mM)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.71 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.8847 mL | 19.4235 mL | 38.8470 mL | |
| 5 mM | 0.7769 mL | 3.8847 mL | 7.7694 mL | |
| 10 mM | 0.3885 mL | 1.9424 mL | 3.8847 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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