| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
Oxolinic acid-d5 is the deuterated form of Oxolinic acid, which is a quinolone antibiotic. Oxolinic acid is effective against both Gram-negative and Gram-positive bacteria and is a DNA/RNA synthesis inhibitor. It can be used for the research of acute and chronic urinary tract infections.
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|---|---|
| ln Vitro |
Drug molecules have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as tracers for quantitation during the drug development process. Due to its potential to alter the pharmacokinetic and metabolic profiles of medications, deuteration has drawn attention[1].
The in vitro activity of Oxolinic acid-d5 mirrors that of Oxolinic acid, which is an antibiotic. The deuterated form is used as an internal standard to accurately quantify Oxolinic acid in cell-based and microbiological experiments. |
| ln Vivo |
In vivo, Oxolinic acid-d5 is used as a tracer to study the pharmacokinetics, residue analysis, and drug metabolism of Oxolinic acid, particularly in veterinary and microbiological research.
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| Enzyme Assay |
The primary application of Oxolinic acid-d5 is as an internal standard in LC-MS/MS or GC-MS analysis. A known amount of the labeled compound is added to biological samples (e.g., plasma, tissue, or milk). Following extraction, the sample is analyzed.
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| Cell Assay |
In cellular or microbiological assays, Oxolinic acid-d5 is used to quantify the concentration of Oxolinic acid. This allows researchers to study its uptake, accumulation, and antibacterial activity.
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| Animal Protocol |
In animal studies, Oxolinic acid-d5 is utilized as an internal standard for the quantification of Oxolinic acid in pharmacokinetic and residue studies. Following administration to animal models, samples are collected and analyzed by LC-MS.
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| ADME/Pharmacokinetics |
Oxolinic acid-d5 has a molecular weight of 266.26 g/mol, compared to 261.23 g/mol for the unlabeled compound.
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| Toxicity/Toxicokinetics |
Oxolinic acid-d5 is intended for research use only and is not for therapeutic or diagnostic use in humans. As a stable isotope, it is non-radioactive and poses minimal radiological risk. Its toxicological profile is expected to be similar to that of Oxolinic acid.
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| References |
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| Additional Infomation |
Oxolinic acid-d5 is a stable isotope-labeled compound used as an internal standard for the bioanalysis of Oxolinic acid. It is a valuable tool for pharmacokinetic, residue analysis, and drug metabolism studies, particularly in veterinary and microbiological research.
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| Molecular Formula |
C13H6D5NO5
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|---|---|
| Molecular Weight |
266.26
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| Exact Mass |
266.095
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| CAS # |
1189890-98-9
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| Related CAS # |
Oxolinic acid;14698-29-4
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| PubChem CID |
46782642
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| Appearance |
White to off-white solid powder
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| Density |
1.5±0.1 g/cm3
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| Boiling Point |
473.2±45.0 °C at 760 mmHg
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| Flash Point |
240.0±28.7 °C
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| Vapour Pressure |
0.0±1.2 mmHg at 25°C
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| Index of Refraction |
1.638
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| LogP |
1.43
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
19
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| Complexity |
446
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[2H]C([2H])([2H])C([2H])([2H])N1C=C(C(=O)C2=CC3=C(C=C21)OCO3)C(=O)O
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| InChi Key |
KYGZCKSPAKDVKC-ZBJDZAJPSA-N
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| InChi Code |
InChI=1S/C13H11NO5/c1-2-14-5-8(13(16)17)12(15)7-3-10-11(4-9(7)14)19-6-18-10/h3-5H,2,6H2,1H3,(H,16,17)/i1D3,2D2
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| Chemical Name |
8-oxo-5-(1,1,2,2,2-pentadeuterioethyl)-[1,3]dioxolo[4,5-g]quinoline-7-carboxylic acid
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| Synonyms |
oxolinic acid d5; Oxolinic acid-d5
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.7557 mL | 18.7786 mL | 37.5573 mL | |
| 5 mM | 0.7511 mL | 3.7557 mL | 7.5115 mL | |
| 10 mM | 0.3756 mL | 1.8779 mL | 3.7557 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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