| Size | Price | Stock | Qty |
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| 1KU |
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| 10KU |
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| Other Sizes |
Purity: Activity: 268780u
| Targets |
The enzymatic target of Nuclease S1 is single-stranded nucleic acids, specifically the phosphodiester bonds of ssDNA and RNA. The enzyme exhibits specificity for single-stranded regions and cleaves both terminal and internal phosphodiester bonds. Nuclease S1 does not efficiently digest double-stranded DNA or DNA-RNA hybrids, making it valuable for selectively removing single-stranded regions from nucleic acid preparations while leaving double-stranded structures intact.
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| ln Vitro |
Nuclease S1 exhibits potent in vitro activity against single-stranded nucleic acid substrates. The enzyme specifically degrades single-stranded oligonucleotides composed of either deoxynucleotides or ribonucleotides. In cell-free systems, Nuclease S1 is used to eliminate non-annealed polynucleotide tails and hairpin loops in DNA-RNA or DNA-DNA duplexes during hybridization studies. The enzyme's activity is optimal at acidic pH (4.6) and 37°C, with a unit definition based on the release of perchloric acid-soluble material from single-stranded nucleic acid substrates.
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| ln Vivo |
Nuclease S1 is an enzyme used in vitro and does not have direct cellular activity in living systems. Its primary applications are in molecular biology research for processing nucleic acid samples. The enzyme can be used to remove single-stranded overhangs from DNA fragments prior to ligation, to map mutational alterations in DNA, and to analyze nucleic acid structure. In cell culture, Nuclease S1 is not typically added directly to living cells but is used to prepare nucleic acid samples extracted from cells.
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| Enzyme Assay |
The standard in vitro assay for Nuclease S1 involves incubation of the enzyme with single-stranded nucleic acid substrate in a buffer containing 30 mM sodium acetate (pH 4.6), 50 mM NaCl, 1 mM ZnSO4, and 5% glycerol. Reactions are typically performed at 37°C for 10-30 minutes. One unit of enzyme activity is defined as the amount that causes 1.0 microgram of single-stranded nucleic acid to become perchloric acid soluble per minute at pH 4.6 at 37°C. The reaction is terminated by addition of EDTA or by heating to 70°C. Digestion products can be analyzed by agarose or polyacrylamide gel electrophoresis to assess the extent of nucleic acid degradation.
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| Cell Assay |
Nuclease S1 is not used directly in cell culture experiments but is employed in the preparation and analysis of nucleic acids from cultured cells. Cells are lysed and nucleic acids are extracted, followed by treatment with Nuclease S1 to remove single-stranded regions. The enzyme can be used to analyze chromatin structure by digesting single-stranded DNA regions in isolated nuclei. In genetic recombination experiments, Nuclease S1 is used to eliminate non-annealed polynucleotide tails and hairpin loops in DNA-DNA duplexes.
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| Animal Protocol |
Nuclease S1 is an enzyme used exclusively in vitro and is not administered to animals. There are no animal experimental protocols for this enzyme as a therapeutic agent. The enzyme is used in research settings to prepare nucleic acid samples from animal tissues or to analyze nucleic acid structure. Tissue samples are homogenized, nucleic acids are extracted, and Nuclease S1 digestion is performed in vitro to remove single-stranded regions for subsequent analysis of DNA structure or for mapping mutational alterations.
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| ADME/Pharmacokinetics |
Nuclease S1 is an enzyme used in biochemical research and does not have pharmacokinetic properties as it is not administered as a drug. When used in vitro, the enzyme is active at acidic pH (4.6) and requires zinc ions for activity. The enzyme is sensitive to high temperatures and is typically inactivated by heating to 70°C or by addition of chelating agents such as EDTA. Storage conditions require -20°C in buffers containing glycerol to maintain enzymatic activity.
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| Toxicity/Toxicokinetics |
Nuclease S1 is a research enzyme and is not intended for therapeutic use, therefore it lacks established toxicity profiles. Standard laboratory safety precautions should be observed when handling the enzyme, including the use of appropriate personal protective equipment. The enzyme is not considered highly toxic but should be handled with care to avoid contact with skin and eyes. As a protein, Nuclease S1 may cause allergic reactions in sensitive individuals. The enzyme is inactivated by heat or by the addition of protein denaturants for disposal.
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| References | |
| Additional Infomation |
Nuclease S1 is a widely used molecular biology reagent for applications including removal of single-stranded overhangs from DNA fragments, mapping of mutational alterations in DNA, analysis of nucleic acid structure, and elimination of non-annealed polynucleotide tails and hairpin loops in hybridization studies. The enzyme is also used in genetic recombination experiments and in the study of DNA-protein interactions. It is isolated from Aspergillus oryzae and is available from multiple commercial suppliers. This product is for research use only and is not for human or veterinary therapeutic applications.
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| Molecular Formula |
C103H206O8
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|---|---|
| Molecular Weight |
1572.73297548294
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| Exact Mass |
1572.574
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| CAS # |
37288-25-8
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| PubChem CID |
161046446
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| Appearance |
Liquid
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| Hydrogen Bond Donor Count |
8
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
25
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| Heavy Atom Count |
111
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| Complexity |
1310
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| Defined Atom Stereocenter Count |
0
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| SMILES |
OC1CCCC(C(C)(C)C)C1CCC.OC1CCC(CCC)C(C1)C(C)(C)C.OC1C(CCC)CCCC1C(C)(C)C.OC1CCC(CCC)CC1C(C)(C)C.OC1CCC(C(C)(C)C)CC1CCC.OC1CC(CCC1CCC)C(C)(C)C.OC1CCC(C)C(C)C1CCCC.OC1CCC(CCC)C(CC(C)C)C1
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| InChi Key |
UBOFXPVXCXUTRL-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/7C13H26O.C12H24O/c1-5-7-10-11(13(2,3)4)8-6-9-12(10)14;1-5-7-10-8-6-9-11(12(10)14)13(2,3)4;1-5-6-10-9-11(13(2,3)4)7-8-12(10)14;1-5-6-10-7-8-11(14)9-12(10)13(2,3)4;1-5-6-10-7-8-11(9-12(10)14)13(2,3)4;1-5-6-10-7-8-12(14)11(9-10)13(2,3)4;1-4-5-11-6-7-13(14)9-12(11)8-10(2)3;1-4-5-6-11-10(3)9(2)7-8-12(11)13/h6*10-12,14H,5-9H2,1-4H3;10-14H,4-9H2,1-3H3;9-13H,4-8H2,1-3H3
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| Chemical Name |
2-butyl-3,4-dimethylcyclohexan-1-ol;2-tert-butyl-4-propylcyclohexan-1-ol;2-tert-butyl-6-propylcyclohexan-1-ol;3-tert-butyl-2-propylcyclohexan-1-ol;3-tert-butyl-4-propylcyclohexan-1-ol;4-tert-butyl-2-propylcyclohexan-1-ol;5-tert-butyl-2-propylcyclohexan-1-ol;3-(2-methylpropyl)-4-propylcyclohexan-1-ol
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| Synonyms |
Nuclease S1
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
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| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.6358 mL | 3.1792 mL | 6.3584 mL | |
| 5 mM | 0.1272 mL | 0.6358 mL | 1.2717 mL | |
| 10 mM | 0.0636 mL | 0.3179 mL | 0.6358 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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