| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
N2-Isobutyryl-2'-O-methylguanosine targets purine nucleoside metabolism pathways. As a purine nucleoside analog, the compound can be incorporated into nucleic acids or interfere with purine metabolism. The 2'-O-methyl modification enhances the stability and properties of the nucleoside. Purine nucleoside analogs have broad antitumor activity.
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|---|---|
| ln Vitro |
In cell-free biochemical systems, this compound is used as a building block for nucleic acid synthesis and modification. The 2'-O-methyl modification enhances the stability of RNA molecules. The isobutyryl protecting group at the N2 position allows for selective deprotection and further functionalization.
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| ln Vivo |
This compound does not exhibit direct cellular activity as it is a protected nucleoside building block. Nucleic acid molecules synthesized using this nucleoside can be delivered into cells for various functional studies. The 2'-O-methyl modification enhances the metabolic stability of nucleic acids in cellular environments.
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| Enzyme Assay |
The standard procedure for using this compound involves its incorporation into nucleic acid sequences during solid-phase synthesis. The protected nucleoside is converted to the phosphoramidite form and coupled to the growing nucleic acid chain. Following chain assembly, the isobutyryl protecting group is removed, and the nucleic acid is deprotected.
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| Cell Assay |
No cell-based experimental protocols are directly applicable to this protected nucleoside as it is a chemical building block. Nucleic acids synthesized using this nucleoside can be evaluated in cell culture experiments. Typical protocols include transfection of modified nucleic acids into mammalian cell lines and assessment of stability and functional effects.
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| Animal Protocol |
This compound is not administered to animals as it is a chemical building block for nucleic acid synthesis. Nucleic acids synthesized using this nucleoside may be evaluated in animal models for therapeutic applications.
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| ADME/Pharmacokinetics |
As a chemical building block, this compound does not have established pharmacokinetic properties. Nucleic acids containing 2'-O-methyl modifications exhibit enhanced metabolic stability. The pharmacokinetic profile depends on the overall nucleic acid sequence and additional modifications.
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| Toxicity/Toxicokinetics |
The compound is not intended for therapeutic use and lacks established toxicity profiles. Standard laboratory safety precautions should be observed when handling this chemical reagent.
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| References | |
| Additional Infomation |
N2-Isobutyryl-2'-O-methylguanosine is a research-grade chemical used in nucleic acid chemistry and molecular biology. It is a purine nucleoside analog with an isobutyryl protecting group at the N2 position and a 2'-O-methyl modification. It is not an approved pharmaceutical and has no clinical trial history. This product is intended for research use only.
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| Molecular Formula |
C15H21N5O6
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|---|---|
| Molecular Weight |
367.36
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| Exact Mass |
367.149
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| CAS # |
63264-29-9
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| PubChem CID |
135536008
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| Appearance |
White to off-white solid powder
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| Density |
1.68±0.1 g/cm3(Predicted)
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| LogP |
-0.1
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
26
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| Complexity |
599
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| Defined Atom Stereocenter Count |
4
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| SMILES |
CC(C)C(=O)NC1=NC2=C(C(=O)N1)N=CN2[C@H]3[C@@H]([C@@H]([C@H](O3)CO)O)OC
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| InChi Key |
RPULCYXEYODQOG-AKAIJSEGSA-N
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| InChi Code |
InChI=1S/C15H21N5O6/c1-6(2)12(23)18-15-17-11-8(13(24)19-15)16-5-20(11)14-10(25-3)9(22)7(4-21)26-14/h5-7,9-10,14,21-22H,4H2,1-3H3,(H2,17,18,19,23,24)/t7-,9-,10-,14-/m1/s1
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| Chemical Name |
N-[9-[(2R,3R,4R,5R)-4-hydroxy-5-(hydroxymethyl)-3-methoxyoxolan-2-yl]-6-oxo-1H-purin-2-yl]-2-methylpropanamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~100 mg/mL (~272.2 mM; with ultrasonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (6.81 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.81 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (6.81 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7221 mL | 13.6106 mL | 27.2213 mL | |
| 5 mM | 0.5444 mL | 2.7221 mL | 5.4443 mL | |
| 10 mM | 0.2722 mL | 1.3611 mL | 2.7221 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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