| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
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| Other Sizes |
| Targets |
Purine nucleoside metabolism pathways; viral DNA polymerase. Guanosine is a purine nucleoside with anti-herpesvirus activity. L-Guanosine, as the L-configuration, may have different biological activity compared to D-guanosine. As a nucleoside analog, it may interfere with nucleic acid metabolism.
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|---|---|
| ln Vitro |
L-Guanosine demonstrates activity as a purine nucleoside in cellular assays. Guanosine has anti-herpesvirus activity. The L-configuration may affect enzyme recognition and activity compared to the natural D-form. Detailed IC50 values have not been disclosed.
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| ln Vivo |
L-Guanosine has anti-herpesvirus activity. As a nucleoside, it would be expected to have activity in animal models of viral infection. In vivo studies would be needed to evaluate its therapeutic potential compared to D-guanosine.
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| Enzyme Assay |
In vitro assays for nucleosides involve measuring incorporation into nucleic acids or enzyme inhibition. For antiviral activity, standard antiviral assays are used. The compound is tested at varying concentrations in virus-infected cells. Viral replication is measured by qPCR or plaque assays.
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| Cell Assay |
Cells (e.g., virus-infected cells) are cultured and treated with L-Guanosine at concentrations typically ranging from 0.1–100 μM for 24–72 hours. Viral replication is measured by qPCR or plaque assays. Cell viability is assessed by standard assays.
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| Animal Protocol |
In vivo animal studies for L-Guanosine have not been extensively reported. The compound is primarily used as a research tool for nucleoside studies. Future studies in animal models of herpesvirus infection would be needed to evaluate in vivo efficacy.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for L-Guanosine have not been published. The compound has a molecular weight of 283.24 and formula C10H13N5O5. As a nucleoside, it is expected to be taken up by cells via nucleoside transporters. In vivo PK parameters have not been characterized.
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| Toxicity/Toxicokinetics |
Toxicology data for L-Guanosine have not been published. The compound is for research use only and not approved for human therapeutic applications. No systematic toxicological evaluation has been performed. Standard safety precautions should be observed.
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| References | |
| Additional Infomation |
L-Guanosine (CAS: 26578-09-6, molecular formula C10H13N5O5, molecular weight 283.24) is the L-configuration of guanosine. Guanosine is a purine nucleoside with anti-herpesvirus activity. It is found in RNA, DNA, and various foods. It is not in clinical trials.
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| Molecular Formula |
C10H13N5O5
|
|---|---|
| Molecular Weight |
283.24
|
| Exact Mass |
283.092
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| CAS # |
26578-09-6
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| PubChem CID |
135484501
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| Appearance |
White to off-white solid powder
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| Density |
2.258 g/cm3
|
| Boiling Point |
775.9±70.0 °C(Predicted)
|
| LogP |
-1.9
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| Hydrogen Bond Donor Count |
5
|
| Hydrogen Bond Acceptor Count |
7
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
20
|
| Complexity |
446
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| Defined Atom Stereocenter Count |
4
|
| SMILES |
C1=NC2=C(N1[C@@H]3[C@H]([C@H]([C@@H](O3)CO)O)O)N=C(NC2=O)N
|
| InChi Key |
NYHBQMYGNKIUIF-GIMIYPNGSA-N
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| InChi Code |
InChI=1S/C10H13N5O5/c11-10-13-7-4(8(19)14-10)12-2-15(7)9-6(18)5(17)3(1-16)20-9/h2-3,5-6,9,16-18H,1H2,(H3,11,13,14,19)/t3-,5-,6-,9-/m0/s1
|
| Chemical Name |
2-amino-9-[(2S,3S,4R,5S)-3,4-dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-1H-purin-6-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.5306 mL | 17.6529 mL | 35.3057 mL | |
| 5 mM | 0.7061 mL | 3.5306 mL | 7.0611 mL | |
| 10 mM | 0.3531 mL | 1.7653 mL | 3.5306 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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