| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
L-Glutamine-15N2 itself does not have a traditional biological target; it is a stable isotope-labeled tracer. The parent compound L-Glutamine is a non-essential amino acid that plays a crucial role in nitrogen metabolism, serving as a nitrogen donor for the synthesis of nucleotides, amino acids, and other nitrogen-containing compounds. L-Glutamine-15N2 is used to track nitrogen flux through metabolic pathways, including the urea cycle, purine and pyrimidine synthesis, and amino acid metabolism.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
L-Glutamine-15N2 does not exhibit direct in vitro biological activity as it is an isotopically labeled tracer. However, the parent compound L-Glutamine is a non-essential amino acid that is involved in many metabolic processes and serves as a carbon source for oxidation in certain cells. In cell culture studies, L-Glutamine-15N2 is used to trace nitrogen metabolic pathways by monitoring the incorporation of ¹⁵N into downstream metabolites using mass spectrometry. |
| ln Vivo |
L-Glutamine-15N2 is not administered as a therapeutic agent; it is used as a metabolic tracer in in vivo studies. When administered to animal models, it allows researchers to track nitrogen flow through various metabolic pathways, including protein synthesis, nucleotide synthesis, and ammonia detoxification. The labeled compound is used to study nitrogen metabolism in healthy and diseased states, including cancer, liver disease, and metabolic disorders.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays for L-Glutamine-15N2 are not applicable, as it is a metabolic tracer rather than a bioactive compound. Quality control assays involve HPLC or mass spectrometry to verify isotopic purity (≥98 atom % ¹⁵N) and chemical purity. The compound is also tested for solubility and stability under recommended storage conditions. It is soluble in water and dilute acids.
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| Cell Assay |
In vitro cellular assays for L-Glutamine-15N2 involve metabolic tracing studies rather than traditional bioactivity assays. Cells are cultured in media containing L-Glutamine-15N2, and the incorporation of ¹⁵N into metabolites such as glutamate, glutamine, and nucleotides is measured by LC-MS or GC-MS. These studies allow researchers to quantify nitrogen flux through various metabolic pathways. The compound is used to study cellular nitrogen metabolism in various cell types.
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| Animal Protocol |
In vivo animal studies for L-Glutamine-15N2 involve metabolic tracing experiments. Animals are administered L-Glutamine-15N2 via oral or intravenous routes. Blood, tissues, and excreta are collected at various time points, and the ¹⁵N enrichment in metabolites is measured by mass spectrometry. These studies provide insights into whole-body nitrogen metabolism, including protein turnover, amino acid metabolism, and nitrogen excretion. They are used in metabolic disease and cancer research.
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| ADME/Pharmacokinetics |
L-Glutamine-15N2 has a molecular formula of C₅H₁₀¹⁵N₂O₃ and a molecular weight of 148.13. The compound appears as a white crystalline powder with a purity of ≥98% and an isotopic enrichment of ≥98 atom % ¹⁵N. It is soluble in water and is stable under recommended storage conditions. The compound is intended for research use only and is not for human consumption.
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| Toxicity/Toxicokinetics |
The toxicity profile of L-Glutamine-15N2 is not extensively characterized, as it is a stable isotope-labeled tracer rather than a therapeutic agent. The parent compound L-Glutamine is a naturally occurring amino acid that is generally recognized as safe. Standard safety data for handling isotopically labeled compounds in laboratory settings would apply. L-Glutamine-15N2 is intended for research use only and is not approved for clinical use.
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| References | |
| Additional Infomation |
L-Glutamine-15N2 (CAS 204451-48-9) is a stable isotope-labeled form of the amino acid L-Glutamine, where two nitrogen atoms are replaced with the isotope nitrogen-15. It has a molecular formula of C₅H₁₀¹⁵N₂O₃ and a molecular weight of 148.13. L-Glutamine is a non-essential amino acid found in large amounts in the human body and is involved in many metabolic processes. This labeled compound is used as a tracer in metabolic studies to track nitrogen flow and utilization. It is intended for research use only and is not approved for clinical use.
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| Molecular Formula |
C5H1015N2O3
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|---|---|
| Molecular Weight |
148.13
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| Exact Mass |
148.063
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| CAS # |
204451-48-9
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| Related CAS # |
L-Glutamine;56-85-9
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| PubChem CID |
16213341
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| Appearance |
White to off-white solid powder
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| Melting Point |
185ºC (dec.)(lit.)
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| LogP |
0.064
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
10
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| Complexity |
146
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| Defined Atom Stereocenter Count |
1
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : 10 mg/mL (67.51 mM)
DMSO : 3.85 mg/mL (25.99 mM ) |
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.7508 mL | 33.7541 mL | 67.5083 mL | |
| 5 mM | 1.3502 mL | 6.7508 mL | 13.5017 mL | |
| 10 mM | 0.6751 mL | 3.3754 mL | 6.7508 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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