| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| Other Sizes |
| Targets |
Not applicable. As a disaccharide standard, L-Digluronic acid disodium does not have a specific pharmacological target. It is used to study hyaluronic acid (hyaluronan) degradation products and as a building block for the synthesis of larger oligosaccharides. It is involved in research on glycosaminoglycan metabolism, particularly the degradation of hyaluronic acid by hyaluronidases, which produce unsaturated disaccharides.
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| ln Vitro |
No in vitro biological activity has been reported. L-Digluronic acid disodium is a disaccharide standard used for analytical and biochemical purposes. It may be used in studies of glycosaminoglycan metabolism, as a substrate or product for enzymes involved in hyaluronic acid degradation, or as a reference standard for the quantification of hyaluronan oligosaccharides by HPLC or LC-MS. It is not a drug and is not used in cell-based assays for therapeutic evaluation.
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| ln Vivo |
No in vivo activity has been reported. As a disaccharide standard, it is not administered as a therapeutic agent. It is used exclusively in analytical chemistry applications for quality control, method development, and as a reference standard in metabolomics and glycosaminoglycan research. It may be used as a substrate or inhibitor in studies of hyaluronidases and other GAG-degrading enzymes.
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| Enzyme Assay |
Not applicable. As an analytical standard, L-Digluronic acid disodium is used in HPLC and LC-MS methods for the analysis of hyaluronic acid and its degradation products. A typical protocol involves dissolving the compound in water or mobile phase (e.g., 50 mM ammonium formate, pH 5.5) to prepare a stock solution of 1-10 mg/mL. Working standards are prepared by serial dilution. For HPLC analysis, 10-20 uL of the standard solution is injected into an HPLC system equipped with a C18 reversed-phase column and UV detection (e.g., 200-230 nm) or CAD (charged aerosol detection). For LC-MS, the compound is analyzed in negative ion mode, and the deprotonated molecule [M-H]- or disodium salt peaks are monitored. Calibration curves are constructed for quantification. No enzyme or receptor binding assays are applicable.
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| Cell Assay |
Not applicable. L-Digluronic acid disodium is not typically used in cell-based assays. It is an analytical standard and disaccharide building block. For studies involving cultured cells, hyaluronan oligosaccharides (including the disaccharide unit) have been shown to have biological effects (e.g., modulation of inflammation, angiogenesis, and cell migration). However, the disodium salt of L-Digluronic acid is primarily an analytical standard, not a test article for cellular studies.
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| Animal Protocol |
No animal studies are conducted with this compound as a test article. It is used as an analytical standard in pharmacokinetic or metabolism studies where hyaluronic acid or its degradation products are being measured in biological samples (e.g., plasma, urine, tissue extracts). The compound is spiked into samples as a standard for calibration or quality control purposes before LC-MS analysis.
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| ADME/Pharmacokinetics |
No pharmacokinetic data has been reported. L-Digluronic acid disodium is a disaccharide (molecular weight 378.22 g/mol as the disodium salt). As a highly polar, anionic carbohydrate, it is not orally bioavailable and is not absorbed systemically. It would be rapidly excreted unchanged in urine if administered intravenously. Its utility is as an analytical standard, not as a drug.
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| Toxicity/Toxicokinetics |
No toxicity data has been reported. L-Digluronic acid disodium is a disaccharide that is a normal component of hyaluronic acid degradation. Glucuronic acid and its disaccharides are generally considered safe and non-toxic. The compound is not intended for human consumption as a pure substance. Standard chemical safety precautions apply.
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| References |
[1]. Spadari CC, et al. Potential Use of Alginate-Based Carriers As Antifungal Delivery System. Front Microbiol. 2017 Jan 30;8:97.
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| Additional Infomation |
L-Digluronic acid disodium (also known as beta-D-GlcpA-(1→4)-D-GlcpA disodium salt) is a disaccharide standard used in hyaluronan (hyaluronic acid) research. Hyaluronic acid is a linear polysaccharide composed of repeating units of D-glucuronic acid and N-acetyl-D-glucosamine. L-Digluronic acid (two glucuronic acid units) is a component of hyaluronan oligosaccharides. The molecular formula is C12H16Na2O13, and the molecular weight is 414.22 (for the disodium salt) or 378.22 for the free acid. It is used as an analytical standard for LC-MS and HPLC methods. It is stored at -20degC. It is not approved for clinical use. It is for research use only.
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| Molecular Formula |
C12H16NA2O13
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|---|---|
| Molecular Weight |
414.227187156677
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| Exact Mass |
414.038
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| CAS # |
1883438-76-3
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| Related CAS # |
L-Diguluronic acid;34044-54-7
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| PubChem CID |
166642517
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
6
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| Hydrogen Bond Acceptor Count |
13
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
27
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| Complexity |
522
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| Defined Atom Stereocenter Count |
10
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| SMILES |
[Na+].[Na+].O([C@H]1[C@H]([C@H]([C@@H]([C@H](C(=O)[O-])O1)O)O)O)[C@@H]1[C@H](C(=O)[O-])O[C@H]([C@H]([C@H]1O)O)O
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| InChi Key |
NTIXLLFKQPXXAX-HXWROTKTSA-L
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| InChi Code |
InChI=1S/C12H18O13.2Na/c13-1-2(14)7(9(18)19)25-12(5(1)17)24-6-3(15)4(16)11(22)23-8(6)10(20)21;;/h1-8,11-17,22H,(H,18,19)(H,20,21);;/q;2*+1/p-2/t1-,2-,3+,4-,5-,6-,7+,8+,11+,12+;;/m0../s1
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| Chemical Name |
disodium;(2R,3S,4S,5S,6R)-6-[(2R,3S,4R,5S,6R)-2-carboxylato-4,5,6-trihydroxyoxan-3-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4141 mL | 12.0706 mL | 24.1412 mL | |
| 5 mM | 0.4828 mL | 2.4141 mL | 4.8282 mL | |
| 10 mM | 0.2414 mL | 1.2071 mL | 2.4141 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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