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KRA-533

Alias: KRA-533; KRA533; KRA 533
Cat No.:V51454 Purity: ≥98%
COA Product Data Instructions for use SDS
KRA-533 is a selective KRAS agonist.
KRA-533 Chemical Structure CAS No.: 10161-87-2
Product category: Ras
This product is for research use only, not for human use. We do not sell to patients.
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BULK INQUIRY
1-708-310-1919 [email protected]
Top Publications Citing lnvivochem Products
Product Description
KRA-533 is a selective KRAS agonist. In cancer cells, constitutively active GTP-bound KRAS accumulates and initiates both apoptotic and autophagic cell death pathways. KRA-533 binds to the GTP/GDP binding pocket in the KRAS protein to prevent GTP cleavage.
Biological Activity I Assay Protocols (From Reference)
Targets
KRAS
Animal Protocol
Nu/Nu nude mice (mutant KRAS xenografts)[1]
0~30 mg/kg
i.p.; 28 days
References

[1]. Small Molecule KRAS Agonist for Mutant KRAS Cancer Therapy [published correction appears in Mol Cancer. 2020 May 20;19(1):93]. Mol Cancer. 2019;18(1):85. Published 2019 Apr 10.

These protocols are for reference only. InvivoChem does not independently validate these methods.
Physicochemical Properties
Molecular Formula
C13H16BRNO3
Molecular Weight
314.175
Exact Mass
313.03
Elemental Analysis
C, 49.70; H, 5.13; Br, 25.43; N, 4.46; O, 15.28
CAS #
10161-87-2
Related CAS #
10161-87-2
Appearance
Solid powder
SMILES
C1=CC(=CC=C1CCCCNC(=O)CBr)C(=O)O
InChi Key
WZQJLTLURXNPQG-UHFFFAOYSA-N
InChi Code
InChI=1S/C13H16BrNO3/c14-9-12(16)15-8-2-1-3-10-4-6-11(7-5-10)13(17)18/h4-7H,1-3,8-9H2,(H,15,16)(H,17,18)
Chemical Name
4-[4-[(2-bromoacetyl)amino]butyl]benzoic acid
Synonyms
KRA-533; KRA533; KRA 533
HS Tariff Code
2934.99.9001
Storage

Powder      -20°C    3 years

                     4°C     2 years

In solvent   -80°C    6 months

                  -20°C    1 month
Shipping Condition
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
Solubility Data
Solubility (In Vitro)
DMSO: ~250 mg/mL (~795.7 mM）
Preparing Stock Solutions 1 mg 5 mg 10 mg
1 mM 3.1829 mL 15.9144 mL 31.8289 mL
5 mM 0.6366 mL 3.1829 mL 6.3658 mL
10 mM 0.3183 mL 1.5914 mL 3.1829 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.

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g/mol
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Note: Chemical formula is case sensitive: C12H18N3O4  c12h18n3o4
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Step 1: Enter information below (Recommended: An additional animal to make allowance for loss during the experiment)
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Calculation results

Working concentration： mg/mL；

Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.

(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
             (2) Be sure to add the solvent(s) in order.

Biological Data

  • KRA-533 activates KRAS in association with growth inhibition of NSCLC cells. Mol Cancer . 2019 Apr 10;18(1):85.

  • KRA-533 directly binds to and activates KRAS in an in vitro cell-free system. Mol Cancer . 2019 Apr 10;18(1):85.

  • K117 site is essential for KRA-533 to bind and activate KRAS. Mol Cancer . 2019 Apr 10;18(1):85.

  • KRA-533 induces KRAS activation leading to apoptotic and autophagic cell death in human lung cancer cells. Mol Cancer . 2019 Apr 10;18(1):85.
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