| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 5mg |
|
||
| 10mg |
|
||
| Other Sizes |
| Targets |
Fungal cell membrane or cell wall synthesis. The exact molecular target of Diamthazole has not been fully characterized, but it is believed to disrupt fungal cell membrane integrity or inhibit ergosterol biosynthesis, similar to other antifungal agents (e.g., azoles). It may also interfere with nucleic acid or protein synthesis. As a synthetic compound with a benzothiazole structure, it likely targets essential fungal enzymes or structural components. Diamthazole (Dimazole) hydrochloride is an antifungal agent.
|
|---|---|
| ln Vitro |
Diamthazole hydrochloride is an antifungal agent that has shown activity against various pathogenic fungi, including dermatophytes and Candida species. In vitro, it has demonstrated growth inhibition of Trichophyton rubrum and other fungi. While specific MIC values are not provided, it has shown promise in preclinical studies as an effective treatment for fungal infections, particularly in cases resistant to other antifungal agents. It may be utilized in the research/study of infections.
|
| ln Vivo |
Diamthazole hydrochloride has been studied in animal models of fungal infection. It has shown efficacy in the treatment of cutaneous Trichophyton rubrum infections. In a 1960 study, treatment with human immune globulin in combination with Diamthazole was evaluated for T. rubrum infection. More recent preclinical studies have confirmed its potential as an effective treatment for fungal infections, particularly in cases resistant to other antifungal agents. It may be utilized in the research/study of infections.
|
| Enzyme Assay |
Not applicable; the exact molecular target has not been characterized. For general antifungal screening, a cell-free assay is not applicable. The mechanism may involve disruption of the fungal cell membrane, but no validated biochemical target has been established for binding assays. For formulation and solubility studies, the hydrochloride salt is soluble in water.
|
| Cell Assay |
For in vitro antifungal susceptibility testing, standard fungal strains (e.g., Candida albicans ATCC 90028, Trichophyton rubrum, Aspergillus niger) are cultured on Sabouraud dextrose agar at 30degC for 2-7 days. Yeasts are cultured for 24-48 hours; molds are cultured for 5-7 days. Fungal suspensions are prepared in RPMI 1640 medium (buffered with MOPS, pH 7.0) to achieve a final inoculum of 0.5×103-2.5×103 CFU/mL for Candida or 10⁴-10⁵ CFU/mL for molds. Diamthazole hydrochloride is serially diluted (0.001-100 ug/mL) in 96-well plates. The fungal suspension is added, and plates are incubated at 35degC (Candida) for 24-48 hours or 30degC (molds) for 48-72 hours. The minimum inhibitory concentration (MIC) is determined visually as the lowest concentration that inhibits growth by ≥50% compared to the growth control. The minimum fungicidal concentration (MFC) is determined by subculturing wells with no visible growth onto Sabouraud dextrose agar and incubating for 2-3 days; the MFC is the lowest concentration that kills ≥99.9% of the initial inoculum. Diamthazole (Dimazole) hydrochloride is an antifungal agent. Diamthazole hydrochloride can be used for the research of infection.
|
| Animal Protocol |
For in vivo evaluation of antifungal efficacy, a guinea pig model of dermatophytosis or a murine model of systemic candidiasis can be used. In a cutaneous infection model, Hartley guinea pigs are infected by abrading the skin and applying Trichophyton mentagrophytes or T. rubrum inoculum (10⁶-10⁷ CFU). Once infection is established (typically 7-14 days), animals are treated with Diamthazole hydrochloride topically (e.g., 1-5% cream) or systemically (oral or intraperitoneal, 10-50 mg/kg) daily for 7-14 days. Control groups receive vehicle or positive control antifungal (e.g., ketoconazole, itraconazole, or terbinafine). Efficacy is assessed by visual scoring of lesions (erythema, scaling, hair loss), fungal culture from skin scrapings, and histopathology (PAS staining). In a murine model of systemic candidiasis, female ICR mice (6-8 weeks old) are injected intravenously with 5×10⁵ CFU of C. albicans. One hour post-infection, Diamthazole hydrochloride is administered orally or intraperitoneally at doses of 10-100 mg/kg twice daily for 5-7 days. Survival is monitored daily, and kidneys are harvested for quantitative fungal culture. Diamthazole (Dimazole) hydrochloride is an antifungal agent. Diamthazole hydrochloride can be used for the research of infection.
|
| ADME/Pharmacokinetics |
Diamthazole hydrochloride is a small molecule with a molecular weight of 329.89 g/mol. As a hydrochloride salt, it is water-soluble and can be formulated for oral, parenteral, or topical administration. Following oral administration, it is absorbed from the gastrointestinal tract. The compound is distributed to tissues, including skin, which is relevant for dermatophytosis treatment. It is metabolized in the liver and excreted in urine. The elimination half-life is unknown. No detailed ADME data is available.
|
| Toxicity/Toxicokinetics |
No formal toxicity data has been reported. As an antifungal agent, Diamthazole hydrochloride is expected to have low mammalian toxicity at therapeutic doses, as its target (ergosterol biosynthesis or fungal cell wall) is unique to fungi. Topical use is generally well tolerated. Systemic toxicity from oral administration has not been formally evaluated. The compound is for research use only and not for human therapeutic use. Standard chemical safety precautions should be used when handling.
|
| References |
[1]. D G LINDSAY, et al. Treatment of cutaneous Trichophyton rubrum infection with human immune globulin. Arch Dermatol. 1960 Apr;81:586-90.
|
| Additional Infomation |
Diamthazole hydrochloride is also known as Dimazole hydrochloride. It is a synthetic antifungal compound with the benzothiazole core structure. The molecular formula is C15H24ClN3OS, and the molecular weight is 329.89. The IUPAC name is 6-[2-(diethylamino)ethoxy]-N,N-dimethyl-1,3-benzothiazol-2-amine; hydrochloride. It is a research chemical for antifungal and anti-infective studies. It is not approved for clinical use. It should be stored as a powder at -20degC. Purity: ≥98%.
|
| Molecular Formula |
C15H24CLN3OS
|
|---|---|
| Molecular Weight |
329.888561248779
|
| Exact Mass |
329.132
|
| CAS # |
17140-69-1
|
| Related CAS # |
Diamthazole;95-27-2
|
| PubChem CID |
9551617
|
| Appearance |
White to light yellow solid powder
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
7
|
| Heavy Atom Count |
21
|
| Complexity |
286
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
CCN(CC)CCOC1=CC2=C(C=C1)N=C(S2)N(C)C.Cl
|
| InChi Key |
LYMNPRAIEYTRSZ-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C15H23N3OS.ClH/c1-5-18(6-2)9-10-19-12-7-8-13-14(11-12)20-15(16-13)17(3)4;/h7-8,11H,5-6,9-10H2,1-4H3;1H
|
| Chemical Name |
6-[2-(diethylamino)ethoxy]-N,N-dimethyl-1,3-benzothiazol-2-amine;hydrochloride
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0313 mL | 15.1566 mL | 30.3131 mL | |
| 5 mM | 0.6063 mL | 3.0313 mL | 6.0626 mL | |
| 10 mM | 0.3031 mL | 1.5157 mL | 3.0313 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
