| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| Other Sizes |
| Targets |
D-Glucose-13C6,d7 itself does not have a traditional biological target; it is a stable isotope-labeled tracer. The parent compound D-Glucose is a primary energy source for cells and serves as a critical signaling molecule in relation to cellular metabolic status and stress response. D-Glucose-13C6,d7 is used to track glucose metabolism, including glycolysis, the pentose phosphate pathway, and glycogen synthesis, through isotopic tracing experiments.
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| ln Vitro |
Drug compounds have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as quantitative tracers while the drugs were being developed. Because deuteration may have an effect on a drug's pharmacokinetics and metabolic properties, it is a cause for concern [1].
D-Glucose-13C6,d7 does not exhibit direct in vitro biological activity as it is an isotopically labeled tracer. However, the parent compound D-Glucose is a carbohydrate sweetener and a critical component of general metabolism. In cell culture studies, D-Glucose-13C6,d7 is used to trace glucose metabolic pathways by monitoring the incorporation of ¹³C and deuterium into downstream metabolites using mass spectrometry. |
| ln Vivo |
D-Glucose-13C6,d7 is not administered as a therapeutic agent; it is used as a metabolic tracer in in vivo studies. When administered to animal models, it allows researchers to track glucose flux through various metabolic pathways, including glycolysis, gluconeogenesis, and the TCA cycle. The dual labeling with ¹³C and deuterium enables more detailed metabolic flux analysis and helps distinguish between different metabolic routes. It is used in diabetes research, metabolic disease studies, and nutritional science.
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| Enzyme Assay |
In vitro enzyme/receptor binding assays for D-Glucose-13C6,d7 are not applicable, as it is a metabolic tracer rather than a bioactive compound. Quality control assays involve HPLC or mass spectrometry to verify isotopic purity (≥98 atom % ¹³C and ≥98 atom % D) and chemical purity. The compound is also tested for solubility and stability under recommended storage conditions. It is soluble in water and other polar solvents.
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| Cell Assay |
In vitro cellular assays for D-Glucose-13C6,d7 involve metabolic tracing studies rather than traditional bioactivity assays. Cells are cultured in media containing D-Glucose-13C6,d7, and the incorporation of ¹³C and deuterium into metabolic intermediates is measured by LC-MS or GC-MS. These studies allow researchers to quantify metabolic flux through glycolysis, the TCA cycle, and other pathways. The compound is used to study cellular metabolism in various cell types, including cancer cells, hepatocytes, and adipocytes.
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| Animal Protocol |
In vivo animal studies for D-Glucose-13C6,d7 involve metabolic tracing experiments. Animals are administered the labeled glucose via oral, intravenous, or intraperitoneal routes. Blood, tissues, and excreta are collected at various time points, and the ¹³C and deuterium enrichment in metabolites is measured by mass spectrometry. These studies provide insights into whole-body glucose metabolism, including glucose uptake, disposal, and conversion to other metabolites. They are used in diabetes and metabolic disease research.
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| ADME/Pharmacokinetics |
D-Glucose-13C6,d7 has a molecular formula of ¹³C₆H₅D₇O₆ and a molecular weight of 193.15. The compound appears as a white to off-white solid powder with a purity of ≥98% and isotopic enrichment of ≥98 atom % ¹³C and ≥98 atom % D. It is soluble in water and is stable under recommended storage conditions. The compound is intended for research use only and is not for human consumption.
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| Toxicity/Toxicokinetics |
The toxicity profile of D-Glucose-13C6,d7 is not extensively characterized, as it is a stable isotope-labeled tracer rather than a therapeutic agent. The parent compound D-Glucose is a naturally occurring sugar that is generally recognized as safe. Standard safety data for handling isotopically labeled compounds in laboratory settings would apply. D-Glucose-13C6,d7 is intended for research use only and is not approved for clinical use.
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| References | |
| Additional Infomation |
Aldose-hexose is a hexose with a (potential) aldehyde group at one end. It is both an aldose and a hexose. D-mannose has been reported in peas (Pisum sativum), and relevant data are available. It is a major energy source for organisms. It exists naturally, in a free state in fruits and other parts of plants. It is used medicinally to replenish body fluids and nutrients. See also: Polydextrose (note moved to); D-allose (note moved to); D-mannose (note moved to)... See more...
D-Glucose-13C6,d7 (CAS 201417-01-8) is a dual-labeled isotopologue of D-Glucose featuring six carbon-13 atoms and seven deuterium atoms. It has a molecular formula of ¹³C₆H₅D₇O₆ and a molecular weight of 193.15. D-Glucose is a monosaccharide that is an important carbohydrate in biology and a critical component of general metabolism. This labeled compound is crucial for studies involving carbohydrate metabolism, tracer experiments, and metabolic flux analysis. It is intended for research use only and is not approved for clinical use. |
| Molecular Formula |
13C6H5D7O6
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|---|---|
| Molecular Weight |
193.15
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| Exact Mass |
193.127
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| CAS # |
201417-01-8
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| Related CAS # |
D-Glucose;50-99-7
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| PubChem CID |
24749
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| Appearance |
White to off-white solid powder
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| Melting Point |
150-152ºC(lit.)
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| LogP |
-2.9
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
12
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| Complexity |
138
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
GZCGUPFRVQAUEE-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C6H12O6/c7-1-3(9)5(11)6(12)4(10)2-8/h1,3-6,8-12H,2H2
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| Chemical Name |
2,3,4,5,6-pentahydroxyhexanal
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : 250 mg/mL (1294.33 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.1773 mL | 25.8866 mL | 51.7732 mL | |
| 5 mM | 1.0355 mL | 5.1773 mL | 10.3546 mL | |
| 10 mM | 0.5177 mL | 2.5887 mL | 5.1773 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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