| Size | Price | Stock | Qty |
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| 1mg |
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| Other Sizes |
| Targets |
Bis-ANS dipotassium is a fluorescent probe that interacts with proteins through its hydrophobic phenyl and naphthyl rings. It binds to tubulin with a Kd of 2 μM. The compound is a potent biphasic modulator of protein liquid-liquid phase separation (LLPS), promoting LLPS at low concentrations. It has been used to label mechanically damaged neurons in acute brain.
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| ln Vitro |
Fluorescent molecules like Bis-ANS have been used to monitor the formation of protein aggregates and to explore exposed hydrophobic regions in proteins. Aniline-containing bivalent naphthalene sulfonate is known as bis-ANS. The TDP-43 low complexity domain (LCD)'s liquid-liquid phase separation is modulated by Bis-ANS[2].
In vitro, Bis-ANS dipotassium is used as a high-affinity non-covalent extrinsic fluorescent dye to analyze protein conformation. It binds to tubulin with a Kd of 2 μM. The compound is a potent biphasic modulator of protein liquid-liquid phase separation (LLPS), promoting LLPS at low concentrations and inhibiting it at higher concentrations. It has been used to label mechanically damaged neurons in acute brain. |
| ln Vivo |
No detailed in vivo activity data has been published for Bis-ANS dipotassium. The compound is primarily used as a research tool for studying protein conformation and liquid-liquid phase separation. It has been used to label mechanically damaged neurons in acute brain. Further studies would be needed to evaluate its in vivo applications.
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| Enzyme Assay |
The binding of Bis-ANS dipotassium to proteins can be assessed using fluorescence spectroscopy. In a typical assay, the compound is incubated with proteins such as tubulin, and the fluorescence intensity is measured. The Kd for tubulin binding is 2 μM. The compound has a maximum excitation at 390 nm. The compound's ability to modulate LLPS can be assessed using microscopy to observe droplet formation.
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| Cell Assay |
The cellular activity of Bis-ANS dipotassium is assessed using cell-based assays for protein conformation and LLPS. Cells are treated with Bis-ANS dipotassium, and protein conformation changes are monitored by fluorescence microscopy. The compound's ability to modulate LLPS can be assessed by observing the formation of protein droplets in cells. The compound has been used to label mechanically damaged neurons in acute brain.
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| Animal Protocol |
No detailed in vivo animal model data has been published for Bis-ANS dipotassium. The compound is primarily used as a research tool for studying protein conformation and LLPS. It has been used to label mechanically damaged neurons in acute brain. Future studies may involve the use of animal models to study protein aggregation and LLPS-related diseases.
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| ADME/Pharmacokinetics |
No detailed pharmacokinetic data has been published for Bis-ANS dipotassium. The compound has a molecular weight of 672.85 g/mol and a molecular formula of C32H22K2N2O6S2. It is typically stored at -20°C, protected from light, dry, and sealed. Further studies would be needed to characterize its absorption, distribution, metabolism, and excretion properties.
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| Toxicity/Toxicokinetics |
No detailed toxicity data has been published for Bis-ANS dipotassium. As a fluorescent probe, it is typically used at low concentrations for research applications. Comprehensive toxicology studies would be required to evaluate its safety profile for potential therapeutic development. The compound is intended for research use only.
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| References | |
| Additional Infomation |
See also: 5,5'-bis(8-(phenylamino)-1-naphthalenesulfonate) (note moved to).
Bis-ANS dipotassium (CAS# 65664-81-5) is a protein fluorescent probe with the molecular formula C32H22K2N2O6S2 and molecular weight 672.85 g/mol. It binds to tubulin with a Kd of 2 μM. The compound is a potent biphasic modulator of protein liquid-liquid phase separation (LLPS). It has a maximum excitation at 390 nm and is used to analyze protein conformation. The compound has been used to label mechanically damaged neurons in acute brain. It has a purity of ≥95%. |
| Molecular Formula |
C32H22K2N2O6S2
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|---|---|
| Molecular Weight |
672.85
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| Exact Mass |
672.019
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| CAS # |
65664-81-5
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| PubChem CID |
16213473
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| Appearance |
Light yellow to yellow solid powder
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| LogP |
9.263
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
44
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| Complexity |
994
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
VWLWTJHKQHRTNC-UHFFFAOYSA-L
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| InChi Code |
InChI=1S/C32H24N2O6S2.2K/c35-41(36,37)29-15-7-13-25-23(17-19-27(31(25)29)33-21-9-3-1-4-10-21)24-18-20-28(34-22-11-5-2-6-12-22)32-26(24)14-8-16-30(32)42(38,39)40;;/h1-20,33-34H,(H,35,36,37)(H,38,39,40);;/q;2*+1/p-2
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| Chemical Name |
dipotassium;8-anilino-5-(4-anilino-5-sulfonatonaphthalen-1-yl)naphthalene-1-sulfonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.4862 mL | 7.4311 mL | 14.8622 mL | |
| 5 mM | 0.2972 mL | 1.4862 mL | 2.9724 mL | |
| 10 mM | 0.1486 mL | 0.7431 mL | 1.4862 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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