| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Targets |
- Andrograpanin may interact with the signaling pathway mediated by stromal cell-derived factor-1α (SDF-1α), potentially involving the SDF-1α receptor (e.g., CXCR4, a common receptor for SDF-1α) [1]
- Andrograpanin exerts anti-inflammatory effects by targeting the p38 mitogen-activated protein kinases (p38 MAPKs) signaling pathway. |
|---|---|
| ln Vitro |
Andrograpanin has anti-inflammatory effects in macrophage cells stimulated by lipopolysaccharide by inhibiting the p38 MAPK signaling pathways[1].
Andrograpanin enhances leukocyte chemotaxis caused by the chemokine SDF-1 in Jurkat, THP-1, and PBL cells[2]. - In vitro chemotaxis assays were performed using human peripheral blood leukocytes (e.g., monocytes or neutrophils). Leukocytes were treated with different concentrations of Andrograpanin (specific concentrations not detailed in the abstract) and stimulated with SDF-1α. The results showed that Andrograpanin significantly enhanced SDF-1α-induced leukocyte chemotaxis, with the enhancement effect being concentration-dependent (specific percentage of enhancement not detailed in the abstract) [1] - In vitro experiments were conducted on lipopolysaccharide (LPS)-stimulated macrophage cells (e.g., RAW264.7 murine macrophages). Macrophages were pretreated with Andrograpanin (specific concentrations not detailed in the abstract) followed by LPS stimulation. The results demonstrated that Andrograpanin significantly reduced the production of pro-inflammatory cytokines, including tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) (specific reduction percentages not detailed in the abstract). Additionally, Western blot analysis revealed that Andrograpanin down-regulated the phosphorylation level of p38 MAPKs (p-p38), thereby inhibiting the activation of the p38 MAPKs signaling pathway [2] |
| Cell Assay |
- For the leukocyte chemotaxis assay: Peripheral blood leukocytes were isolated from human donors. Isolated leukocytes were resuspended in appropriate culture medium and divided into different groups: control group (without Andrograpanin), SDF-1α stimulation group, and Andrograpanin + SDF-1α co-treatment groups (with different concentrations of Andrograpanin). The cells were then added to the upper chamber of a transwell plate, and medium containing SDF-1α was added to the lower chamber. After incubation for a specific period (not detailed in the abstract) at 37°C with 5% CO₂, the number of leukocytes that migrated to the lower chamber was counted under a microscope to evaluate chemotaxis ability [1]
- For the macrophage inflammation assay: Murine macrophage cells (e.g., RAW264.7) were cultured in complete medium until reaching logarithmic growth phase. The cells were then seeded into culture plates and allowed to adhere overnight. Next, the cells were pretreated with different concentrations of Andrograpanin for a specific time (not detailed in the abstract), followed by stimulation with LPS (specific concentration not detailed) for another period. After treatment, the culture supernatant was collected to detect the levels of pro-inflammatory cytokines (TNF-α, IL-6) using enzyme-linked immunosorbent assay (ELISA). Additionally, the cells were lysed to extract total proteins, and Western blot analysis was performed to detect the phosphorylation level of p38 MAPKs (using specific primary antibodies against p-p38 and total p38) [2] |
| References |
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| Additional Infomation |
It has been reported that andrograpanin exists in Andrographis affinis, Andrographis paniculata and Potamogeton natans, and there are related data reports.
- Andrograpanin is a natural compound isolated from Andrographis paniculata, a traditional Chinese medicine with a clear anti-inflammatory effect. This study focuses on the effect of andrograpanin on leukocyte chemotaxis and reveals its potential mechanism in regulating immune cell migration (inflammation and immune response-related processes) [1]. - Andrograpanin is derived from Andrographis paniculata, a traditional anti-inflammatory herb. Reference [2] further elucidates the anti-inflammatory mechanism of Andrograpanin: it inhibits the activation of the p38 MAPK signaling pathway in macrophages induced by LPS, thereby reducing the secretion of pro-inflammatory cytokines and alleviating the inflammatory response [2]. |
| Molecular Formula |
C20H30O3
|
|---|---|
| Molecular Weight |
318.4504
|
| Exact Mass |
318.219
|
| CAS # |
82209-74-3
|
| Related CAS # |
82209-74-3
|
| PubChem CID |
11666871
|
| Appearance |
White to off-white solid
|
| Density |
1.09
|
| Melting Point |
106-107ºC
|
| LogP |
4.021
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
4
|
| Heavy Atom Count |
23
|
| Complexity |
535
|
| Defined Atom Stereocenter Count |
4
|
| SMILES |
C[C@]12[C@H](CCC3=CCOC3=O)C(=C)CC[C@@H]1[C@](CCC2)(C)CO
|
| InChi Key |
WKKBRRFSRMDTJB-JYBIWHBTSA-N
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| InChi Code |
InChI=1S/C20H30O3/c1-14-5-8-17-19(2,13-21)10-4-11-20(17,3)16(14)7-6-15-9-12-23-18(15)22/h9,16-17,21H,1,4-8,10-13H2,2-3H3/t16-,17-,19+,20+/m1/s1
|
| Chemical Name |
4-[2-[(1R,4aS,5R,8aS)-5-(hydroxymethyl)-5,8a-dimethyl-2-methylidene-3,4,4a,6,7,8-hexahydro-1H-naphthalen-1-yl]ethyl]-2H-furan-5-one
|
| Synonyms |
Andrograpanin
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: ~100 mg/mL (~314.0 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1402 mL | 15.7011 mL | 31.4021 mL | |
| 5 mM | 0.6280 mL | 3.1402 mL | 6.2804 mL | |
| 10 mM | 0.3140 mL | 1.5701 mL | 3.1402 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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