| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
|
||
| 10mg |
|
||
| 50mg |
|
||
| 100mg |
|
||
| Other Sizes |
| Targets |
7-Iodo-2',3'-dideoxy-7-deaza-guanosine does not have a defined biological target as it is a chemical reagent for DNA synthesis and sequencing. Its role is to serve as a building block or terminator in DNA synthesis reactions. The compound is a dideoxynucleoside that can be incorporated into DNA strands during synthesis, where it acts as a chain terminator due to the lack of 3'-hydroxyl group.
|
|---|---|
| ln Vitro |
In cell-free biochemical systems, this compound is used in DNA synthesis and sequencing reactions. As a dideoxynucleoside, it can be incorporated into DNA strands by DNA polymerases, where it terminates chain elongation. This property makes it useful for DNA sequencing and other applications where chain termination is desired. The iodine substitution at the 7-position may also allow for detection or labeling.
|
| ln Vivo |
This compound does not exhibit direct cellular activity as it is a chemical reagent for DNA synthesis. It can be used in cell-free systems for DNA sequencing and synthesis. In cellular contexts, the compound may be incorporated into DNA if taken up by cells, potentially leading to chain termination and inhibition of DNA synthesis. However, it is primarily used as a research tool.
|
| Enzyme Assay |
The standard procedure for using this compound involves its incorporation into DNA during in vitro DNA synthesis reactions. It is used as a dideoxynucleotide terminator in Sanger sequencing or other chain termination applications. The compound is added to DNA polymerase reactions along with other dNTPs and a DNA template. The resulting DNA fragments are analyzed by gel electrophoresis or capillary electrophoresis.
|
| Cell Assay |
No cell-based experimental protocols are directly applicable to this dideoxynucleoside as it is a chemical reagent for DNA synthesis. The compound can be used in cell-based assays to study DNA synthesis inhibition, but it is primarily used in cell-free systems. In cell culture, the compound could potentially be used to study the effects of DNA chain termination on cell proliferation.
|
| Animal Protocol |
7-Iodo-2',3'-dideoxy-7-deaza-guanosine is not administered to animals as it is a chemical reagent for DNA synthesis. The compound is used exclusively in vitro for DNA sequencing and synthesis applications. It is not intended for therapeutic use and is not administered to animals for pharmacological studies.
|
| ADME/Pharmacokinetics |
As a chemical reagent, this compound does not have established pharmacokinetic properties. The compound is used in vitro and is not intended for systemic administration. Its stability and solubility in aqueous solutions are relevant for its use in DNA synthesis reactions. The compound is typically stored at 4°C, protected from light.
|
| Toxicity/Toxicokinetics |
The compound is not intended for therapeutic use and lacks established toxicity profiles. Standard laboratory safety precautions should be observed when handling this chemical reagent. As a dideoxynucleoside, it may have effects on DNA synthesis if incorporated into cellular DNA. However, it is primarily used as a research tool and is not intended for human use.
|
| References | |
| Additional Infomation |
7-Iodo-2',3'-dideoxy-7-deaza-guanosine is a research-grade chemical supplied for DNA synthesis and sequencing applications. It is not an approved pharmaceutical and has no clinical trial history. The compound is a dideoxynucleoside used in DNA synthesis and sequencing reactions. This product is intended for research use only and is not for human therapeutic applications.
|
| Molecular Formula |
C11H13IN4O3
|
|---|---|
| Molecular Weight |
376.15
|
| Exact Mass |
376.003
|
| CAS # |
114748-67-3
|
| PubChem CID |
135577906
|
| Appearance |
Off-white to light yellow solid powder
|
| Density |
2.4±0.1 g/cm3
|
| Index of Refraction |
1.882
|
| LogP |
-0.4
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
19
|
| Complexity |
419
|
| Defined Atom Stereocenter Count |
2
|
| SMILES |
C1C[C@@H](O[C@@H]1CO)N2C=C(C3=C2N=C(NC3=O)N)I
|
| InChi Key |
KJNOBXXCZQLWLF-CAHLUQPWSA-N
|
| InChi Code |
InChI=1S/C11H13IN4O3/c12-6-3-16(7-2-1-5(4-17)19-7)9-8(6)10(18)15-11(13)14-9/h3,5,7,17H,1-2,4H2,(H3,13,14,15,18)/t5-,7+/m0/s1
|
| Chemical Name |
2-amino-7-[(2R,5S)-5-(hydroxymethyl)oxolan-2-yl]-5-iodo-3H-pyrrolo[2,3-d]pyrimidin-4-one
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO : ~125 mg/mL (~332.31 mM)
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6585 mL | 13.2926 mL | 26.5851 mL | |
| 5 mM | 0.5317 mL | 2.6585 mL | 5.3170 mL | |
| 10 mM | 0.2659 mL | 1.3293 mL | 2.6585 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
