| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
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| Other Sizes |
| Targets |
The primary target of 5-Galloylquinic acid is reactive oxygen species (ROS), for which it acts as a scavenger. By scavenging ROS, the compound exerts antioxidant activity and protects cells from oxidative damage. It may also target Leishmania parasites, demonstrating anti-leishmanial activity. These targets make it relevant for research on oxidative stress, antioxidant defense, and infectious diseases.
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| ln Vitro |
5-galloylquinic acid, (−)-epigallocatechin-3-O-gallate, and (−)-epicatechin-3-O-gallate are the three primary ROS scavengers found in green tea. acid ester [1].
In vitro, 5-Galloylquinic acid demonstrates potent ROS scavenging activity. It is one of the three primary ROS scavengers found in green tea. The compound exhibits antioxidant activity and anti-leishmanial bioactivities. It is studied in vitro to understand its mechanisms of antioxidant action and its potential protective effects against oxidative stress. These in vitro activities support its use in antioxidant and infectious disease research. |
| ln Vivo |
In vivo, 5-Galloylquinic acid is found in green tea and other plants. It functions as a dietary antioxidant that may contribute to the health benefits associated with green tea consumption. The compound's antioxidant activity suggests potential protective effects against oxidative stress-related diseases. However, detailed in vivo efficacy data are limited. Further studies are needed to evaluate its therapeutic potential in animal models of oxidative stress and infectious diseases.
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| Enzyme Assay |
In vitro antioxidant assays for 5-Galloylquinic acid include DPPH, ABTS, and FRAP radical scavenging assays. The compound is incubated with radical-generating systems at concentrations ranging from 0.1-1000 μM, and scavenging activity is measured spectrophotometrically. ROS scavenging activity is assessed using cell-free or cell-based ROS detection assays. Anti-leishmanial activity is assessed using Leishmania parasite cultures. All assays include appropriate controls and reference compounds (e.g., Trolox, ascorbic acid).
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| Cell Assay |
In vitro cell-based assays for 5-Galloylquinic acid are conducted using various cell lines to assess antioxidant and cytoprotective effects. Cells are treated with compound concentrations ranging from 0.1-1000 μM for 24-72 hours, followed by oxidative stress induction. ROS levels are measured using fluorescent probes. Cell viability is assessed using MTT assays. Anti-leishmanial activity is assessed using infected macrophage cultures. Experiments include vehicle controls and positive controls.
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| Animal Protocol |
In vivo animal studies with 5-Galloylquinic acid are limited, as the compound is primarily studied as a dietary antioxidant. Studies may be conducted in models of oxidative stress, inflammation, or infectious diseases. The compound is administered via oral or intraperitoneal routes at doses ranging from 1-100 mg/kg. Oxidative stress markers and antioxidant enzyme activities are measured. Each group consists of 6-10 animals with vehicle-treated controls. Further studies are needed for comprehensive characterization.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of 5-Galloylquinic acid have not been extensively characterized. As a polar, glycosylated phenolic compound, it is expected to have moderate oral bioavailability. The compound is found in green tea and other plants and likely undergoes metabolism through hydrolysis and conjugation, with elimination via renal excretion. Detailed PK parameters such as half-life, Cmax, and AUC require further investigation.
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| Toxicity/Toxicokinetics |
Toxicological data for 5-Galloylquinic acid indicate that it is generally well-tolerated, as it is a natural compound found in green tea and other plants. No significant toxicity has been reported at typical dietary exposure levels. However, comprehensive toxicological studies have not been conducted. As with all research chemicals, appropriate safety precautions should be taken during handling.
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| References | |
| Additional Infomation |
According to reports, 5-galloylquinic acid has been found in Castanopsis fissa, Arbutus unedo, and Quercus salicina, and relevant data are available for reference.
5-Galloylquinic acid is the main ROS scavenger in green tea and has potent antioxidant activity. It is one of the three primary ROS scavengers found in green tea and is used as an index for the baking intensity of oolong teas. The compound also has anti-leishmanial activity. It is used in research on oxidative stress, antioxidant defense, and infectious diseases. Not approved for clinical therapeutic use; intended for research purposes. |
| Molecular Formula |
C14H16O10
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|---|---|
| Molecular Weight |
344.270845413208
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| Exact Mass |
344.074
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| CAS # |
53584-43-3
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| Related CAS # |
Theogallin;17365-11-6
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| PubChem CID |
14520970
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| Appearance |
White to off-white solid powder
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| Density |
1.9±0.1 g/cm3
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| Boiling Point |
754.4±60.0 °C at 760 mmHg
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| Flash Point |
283.0±26.4 °C
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| Vapour Pressure |
0.0±2.7 mmHg at 25°C
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| Index of Refraction |
1.733
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| Source |
Originated from plants: other families
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| LogP |
-0.01
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| Hydrogen Bond Donor Count |
7
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| Hydrogen Bond Acceptor Count |
10
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
24
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| Complexity |
484
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| Defined Atom Stereocenter Count |
4
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| SMILES |
C1[C@H]([C@@H]([C@@H](C[C@]1(C(=O)O)O)OC(=O)C2=CC(=C(C(=C2)O)O)O)O)O
|
| InChi Key |
LDPLFHGGZNSKDS-AOGLXQGOSA-N
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| InChi Code |
InChI=1S/C14H16O10/c15-6-1-5(2-7(16)10(6)18)12(20)24-9-4-14(23,13(21)22)3-8(17)11(9)19/h1-2,8-9,11,15-19,23H,3-4H2,(H,21,22)/t8-,9-,11+,14-/m1/s1
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| Chemical Name |
(1R,3R,4S,5R)-1,3,4-trihydroxy-5-(3,4,5-trihydroxybenzoyl)oxycyclohexane-1-carboxylic acid
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| Synonyms |
5Galloylquinic acid; 5 Galloylquinic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9047 mL | 14.5235 mL | 29.0470 mL | |
| 5 mM | 0.5809 mL | 2.9047 mL | 5.8094 mL | |
| 10 mM | 0.2905 mL | 1.4523 mL | 2.9047 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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