| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| 5mg |
|
||
| 10mg |
|
||
| Other Sizes |
|
| ln Vitro |
23-epi-26-Deoxyactein (DA: 10 μM and 20 μM) inhibits 3T3-L1 adipogenesis by downregulating the expression of the essential adipogenic transcription factors, C/ebpα, C/ebpβ, and Pparγ[1]. 23-epi-26-Deoxyactein (DA) shields osteoblasts from Antimycin A-induced cell damage and stimulates mitochondrial biogenesis in pancreatic β-cells to prevent methylglyoxal-induced oxidative cell damage[2]. With an IC50 of 21μM, 23-epi-26-Deoxyactein (DA) causes cell cycle arrest in G1 and suppresses the development of MCF7 human breast cancer cells[3]. Antimycin A-induced cell damage is prevented in osteoblasts by 23-epi-26-Deoxyactein (0.1-1 μM)[4].
|
|---|---|
| ln Vivo |
In HFD-fed rats, 23-epi-26-Deoxyactein (DA: 5 and 10 mg/kg/d) significantly reduces liver weight, fat mass, and body weight increase. In HFD-fed animals, 23-epi-26-Deoxyactein (DA) also lowers blood lipoprotein levels and insulin resistance[1]. 23-epi-26-Deoxyactein (DA) stimulates the SIRT1-FOXO1 pathway and AMPK signaling in mice, which in turn enhances adipocyte lipolysis[1].
|
| Cell Assay |
Cell Differentiation Assay[1]
Cell Types: 3T3-L1 preadipocytes. Tested Concentrations: 0-50 μM. Incubation Duration: 8 days. Experimental Results: 10 μM DA inhibited the adipogenesis of 3T3-L1 preadipocytes mainly at the early stage of differentiation. |
| Animal Protocol |
Animal/Disease Models: Diet induced obesity in C57BL/6 mice[1].
Doses: 1-10 mg/kg. Route of Administration: Orally, daily for 12 weeks. Experimental Results: Lowered body weight gain, fat mass, and liver weight. 5 mg/kg/d DA Dramatically improved HFD-induced glucose intolerance. |
| References |
[1]. Jingjing Yuan, et al. Effects of 23-epi-26-deoxyactein on adipogenesis in 3T3-L1 preadipocytes and diet-induced obesity in C57BL/6 mice. Phytomedicine. 2020 Jun 5;76:153264.
[2]. Kwang Sik Suh, et al. Deoxyactein protects pancreatic β-cells against methylglyoxal-induced oxidative cell damage by the upregulation of mitochondrial biogenesis. Int J Mol Med. 2017 Aug;40(2):539-548. [3]. Einbond, L.S., et al. Growth inhibitory activity of extracts and purified components of black cohosh on human breast cancer cells. Breast Cancer Res. Treat. 83, 221–231. Breast Cancer Res Treat. 2004 Feb;83(3):221-31. [4]. Eun Mi Choi, et al. Deoxyactein Isolated from Cimicifuga racemosa protects osteoblastic MC3T3-E1 cells against antimycin A-induced cytotoxicity. J Appl Toxicol. 2013 Jun;33(6):488-94. |
| Molecular Formula |
C37H56O10
|
|---|---|
| Molecular Weight |
660.83
|
| CAS # |
501938-01-8
|
| SMILES |
O(C(C)=O)[C@@H]1C[C@]23[C@@H](CC[C@H]4C(C)(C)[C@H](CC[C@]24C3)O[C@H]2[C@@H]([C@H]([C@@H](CO2)O)O)O)[C@]2(C)C[C@H]3[C@H]([C@H](C)C[C@]4([C@H]5[C@@](C)(CO4)O5)O3)[C@]21C
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
|---|
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.5132 mL | 7.5662 mL | 15.1325 mL | |
| 5 mM | 0.3026 mL | 1.5132 mL | 3.0265 mL | |
| 10 mM | 0.1513 mL | 0.7566 mL | 1.5132 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
