| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
55 μM (HepG2 cells)[1]
2-Hydroxy-3-methylanthraquinone targets multiple cellular pathways involved in cell proliferation, apoptosis, and microbial growth. As an anthraquinone, it can intercalate into DNA and inhibit topoisomerase enzymes. The compound also exhibits antimicrobial activity against various bacterial and fungal pathogens. Its biological effects are related to its ability to generate reactive oxygen species and modulate cellular signaling pathways. |
|---|---|
| ln Vitro |
In cell-free biochemical systems, 2-Hydroxy-3-methylanthraquinone exhibits antimicrobial activity as evaluated by standard disk diffusion or microdilution assays. The compound's ability to inhibit topoisomerase enzymes can be assessed using cell-free enzymatic assays. Its DNA intercalation activity can be studied using spectroscopic methods.
|
| ln Vivo |
In cell-based assays, 2-Hydroxy-3-methylanthraquinone exhibits anticancer effects by inhibiting cell proliferation and inducing apoptosis. The compound's effects are evaluated in cancer cell lines by measuring cell viability, proliferation, and apoptosis. Its antimicrobial effects are assessed in infected cell cultures.
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| Enzyme Assay |
The cell-free assay for antimicrobial activity involves standard disk diffusion or microdilution methods. The compound is applied to agar plates inoculated with bacterial or fungal strains, and the zone of inhibition is measured. The minimum inhibitory concentration (MIC) is determined from broth microdilution assays. Topoisomerase inhibition can be assessed using purified topoisomerase enzymes and DNA substrates.
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| Cell Assay |
Cell-based assays for 2-Hydroxy-3-methylanthraquinone involve culturing cancer cell lines and treating them with the compound at concentrations ranging from 0.1 to 100 μM. Cell viability is assessed using MTT or CCK-8 assays. Apoptosis is evaluated by Annexin V staining or caspase activity assays. Cell cycle analysis is performed by flow cytometry. The compound's effects on DNA synthesis and topoisomerase activity are assessed.
|
| Animal Protocol |
There is no established animal experimental protocol for 2-Hydroxy-3-methylanthraquinone specifically. As an anthraquinone with potential anticancer and antimicrobial activities, the compound could potentially be evaluated in animal models of cancer and infection. Typical studies involve administration of the compound to mice via oral or intraperitoneal routes.
|
| ADME/Pharmacokinetics |
Pharmacokinetic properties of 2-Hydroxy-3-methylanthraquinone have not been extensively reported. As an anthraquinone with a molecular weight of approximately 238.2 g/mol, the compound would be expected to have moderate bioavailability and tissue penetration. Further pharmacokinetic studies would be required for therapeutic development.
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| Toxicity/Toxicokinetics |
2-Hydroxy-3-methylanthraquinone is intended for research use only and lacks established toxicity profiles for therapeutic applications. Standard laboratory safety precautions should be observed when handling this chemical reagent. As an anthraquinone, the compound may have genotoxic and cytotoxic effects. Standard toxicity studies would be required for therapeutic development.
|
| References | |
| Additional Infomation |
2-Hydroxy-3-methylanthraquinone has been reported in Oldenlandia herbacea var. herbacea, Oldenlandia diffusa, and other organisms with available data.
2-Hydroxy-3-methylanthraquinone is a research-grade chemical supplied as a reference standard for emodin impurity analysis. It is not an approved pharmaceutical and has no clinical trial history. The compound is also known as emodin impurity 16. This product is intended for research and analytical use only. |
| Molecular Formula |
C15H10O3
|
|---|---|
| Molecular Weight |
238.24
|
| Exact Mass |
238.062
|
| CAS # |
17241-40-6
|
| PubChem CID |
10889963
|
| Appearance |
Typically exists as solid at room temperature
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| Density |
1.4±0.1 g/cm3
|
| Boiling Point |
439.7±34.0 °C at 760 mmHg
|
| Flash Point |
233.8±22.2 °C
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| Vapour Pressure |
0.0±1.1 mmHg at 25°C
|
| Index of Refraction |
1.676
|
| LogP |
3.83
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
0
|
| Heavy Atom Count |
18
|
| Complexity |
376
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
CC1=CC2=C(C=C1O)C(=O)C3=CC=CC=C3C2=O
|
| InChi Key |
RNJHIYAJJKOFIO-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C15H10O3/c1-8-6-11-12(7-13(8)16)15(18)10-5-3-2-4-9(10)14(11)17/h2-7,16H,1H3
|
| Chemical Name |
2-hydroxy-3-methylanthracene-9,10-dione
|
| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.1974 mL | 20.9872 mL | 41.9745 mL | |
| 5 mM | 0.8395 mL | 4.1974 mL | 8.3949 mL | |
| 10 mM | 0.4197 mL | 2.0987 mL | 4.1974 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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