| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
The primary target of 2,4'-dichloroacetanilide is fungi. The compound has antifungal activity. It inhibits Trichophyton asteroides with a MIC value of 6.25 µg/mL. The precise molecular targets and mechanisms of action have not been fully elucidated. Further studies are needed to identify its specific targets.
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|---|---|
| ln Vitro |
In vitro studies have demonstrated the antifungal activity of 2,4'-dichloroacetanilide. The compound inhibits T. asteroides with a MIC of 6.25 µg/mL. 2,4'-Dichloroacetanilide is a fungistatic agent. Its in vitro activity supports its potential for further investigation.
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| ln Vivo |
In vivo activity of 2,4'-dichloroacetanilide has not been extensively reported. The compound is primarily used as a research tool for in vitro studies. Animal model studies evaluating its in vivo efficacy have not been extensively published. Further studies are needed to assess its therapeutic potential.
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| Enzyme Assay |
For antifungal activity screening, standard agar diffusion or broth microdilution methods are used. Fungi such as Trichophyton asteroides are cultured on appropriate media and exposed to serial dilutions of 2,4'-dichloroacetanilide. MIC values are determined after 24-48 hours of incubation. Standard protocols for antifungal activity testing are described in the literature.
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| Cell Assay |
For in vitro cell-based studies, fungal cells are cultured in appropriate media and treated with serial dilutions of 2,4'-dichloroacetanilide. Fungal growth is assessed. Cytotoxicity against mammalian cells may be assessed in parallel.
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| Animal Protocol |
In vivo animal studies for 2,4'-dichloroacetanilide have not been extensively reported. For similar antifungal compounds, mouse models of fungal infection may be used. The compound would be administered via oral or intraperitoneal routes. Standard protocols for in vivo efficacy studies can be adapted from the literature.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of 2,4'-dichloroacetanilide have not been comprehensively characterized. As a small molecule with a molecular weight of 204.05 g/mol, it is expected to have reasonable membrane permeability. The compound has a melting point of 168-170 °C. Specific PK parameters such as half-life and bioavailability have not been reported.
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| Toxicity/Toxicokinetics |
Toxicological data for 2,4'-dichloroacetanilide are limited. As a research compound, standard toxicological studies have not been extensively published. Cytotoxicity against mammalian cell lines may be assessed in parallel with activity studies. The compound is for research use only and is not intended for human therapeutic use.
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| References |
[1]. Yamazoe H, et al. [Studies on the experimental chemotherapy of dermatomycosis and candidiasis. V. On the antifungal activity for various arylhaloacetamide compounds in vitro and in vivo]. Yakugaku Zasshi. 1968 Dec;88(12):1602-9. Japanese.
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| Additional Infomation |
2,4'-Dichloroacetanilide is a research compound used in studies of antifungal agents. It has antifungal activity and inhibits T. asteroides with a MIC of 6.25 µg/mL. The compound is a fungistatic agent. No clinical trials or therapeutic applications have been reported.
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| Molecular Formula |
C8H7CL2NO
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|---|---|
| Molecular Weight |
204.05
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| Exact Mass |
202.99
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| CAS # |
3289-75-6
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| PubChem CID |
94920
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| Appearance |
Light brown to brown solid
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| Density |
1.4±0.1 g/cm3
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| Boiling Point |
368.8±27.0 °C at 760 mmHg
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| Melting Point |
168ºC
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| Flash Point |
176.8±23.7 °C
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| Vapour Pressure |
0.0±0.8 mmHg at 25°C
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| Index of Refraction |
1.606
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| LogP |
2.6
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
12
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| Complexity |
155
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1=C(C=CC(=C1)NC(=O)CCl)Cl
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| InChi Key |
UDRCRMHFHHTVSN-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C8H7Cl2NO/c9-5-8(12)11-7-3-1-6(10)2-4-7/h1-4H,5H2,(H,11,12)
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| Chemical Name |
2-chloro-N-(4-chlorophenyl)acetamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~100 mg/mL (~490.1 mM; with ultrasonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (12.25 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (12.25 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.9008 mL | 24.5038 mL | 49.0076 mL | |
| 5 mM | 0.9802 mL | 4.9008 mL | 9.8015 mL | |
| 10 mM | 0.4901 mL | 2.4504 mL | 4.9008 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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