| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| 50mg |
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| ln Vitro |
ZINC69391 has an IC50 ranging from 41 to 54 μM, which inhibits the development of U937, HL-60, KG1A, and Jurkat cells[1]. The enzymatic activity of caspase 3 is enhanced in a concentration-dependent manner by ZINC69391 (50-100 μM; 24 hours) [1]. ZINC69391 (0-125 μM; 72h) inhibits human glioma cell growth [2]. ZINC69391 causes cell cycle arrest at 50–100 μM for 48 hours [2]. Human acute leukemia cells undergo apoptosis in response to ZINC69391 (50 μM; 24 hours) [1].
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| ln Vivo |
In syngeneic animal models, ZINC69391 (25 mg/kg; i.p.; once daily for 21 days) inhibits the colonization of the lung that metastasizes [3].
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| Cell Assay |
Cell proliferation assay[2]
Cell Types: U-87 MG, LN229 Cell Tested Concentrations: 0-125μM Incubation Duration: 72 hrs (hours) Experimental Results: Cell proliferation diminished in a concentration-dependent manner. Cell cycle analysis[2] Cell Types: LN229 Cell Tested Concentrations: 50, 100 μM Incubation Duration: 48 hrs (hours) Experimental Results: The percentage of sub-G0/G1 phase cells increased Dramatically in a concentration-dependent manner. Apoptosis analysis[1] Cell Types: HL-60, U937 and KG1A Cell lines Tested Concentrations: 50 μM Incubation Duration: 24 hrs (hours) Experimental Results: Resulted in a significant increase in apoptotic cells. |
| Animal Protocol |
Animal/Disease Models: Specific pathogen-free female BALB/c inbred mice (carrying F3II cells) [3]
Doses: 25 mg/kg body weight Route of Administration: intraperitoneal (ip) injection; one time/day for 21 days Experimental Results: Total number of lung metastatic colonies The formation is Dramatically diminished by approximately 60%. |
| References |
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| Molecular Formula |
C14H14F3N5
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|---|---|
| Molecular Weight |
309.289672374725
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| Exact Mass |
309.12
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| CAS # |
303094-67-9
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| PubChem CID |
5728890
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| Appearance |
White to off-white solid powder
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| LogP |
2.7
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
22
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| Complexity |
388
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC1=CC(=NC(=N1)/N=C(\N)/NC2=CC=CC=C2C(F)(F)F)C
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| InChi Key |
BEZGMANANMSUSQ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C14H14F3N5/c1-8-7-9(2)20-13(19-8)22-12(18)21-11-6-4-3-5-10(11)14(15,16)17/h3-7H,1-2H3,(H3,18,19,20,21,22)
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| Chemical Name |
2-(4,6-dimethylpyrimidin-2-yl)-1-[2-(trifluoromethyl)phenyl]guanidine
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| Synonyms |
ZINC69391 ZINC-69391 ZINC 69391
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~25 mg/mL (~80.83 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.2332 mL | 16.1661 mL | 32.3321 mL | |
| 5 mM | 0.6466 mL | 3.2332 mL | 6.4664 mL | |
| 10 mM | 0.3233 mL | 1.6166 mL | 3.2332 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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