| Size | Price | Stock | Qty |
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| 1mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| ln Vitro |
Close-range IC50 values of 90, 170, 46, 45, and 79 nM were observed for YM-53601 squalene synthase activity in liver microsomes from hamsters, guinea pigs, rhesus monkeys, and human HepG2 cells[1]. YM-53601 also inhibits hamster delta squalene synthase, which converts [3H] farnesyl diphosphate into [3H] squalene, with an IC50 of 170 nM [2]. In H35 cells, YM-53601 (1 μM) increases thapsigargin cooking, while in HepG2 and H35 cells, it decreases cooking levels [4]. Assay for Cell Viability[4]
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| ln Vivo |
Suspension biosynthesis using YM-53601 suspension solution (ED50, 32 mg/kg)[1]. In addition, YM-53601 decreased curtain non-HDL cholesterol levels in hamsters by almost 70% over the course of five days at a dose of 50 mg/kg/day [2]. In vivo doxorubicin-induced hepatocellular carcinoma (HCC) development and cell death are enhanced by YM-53601 [4].
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| Cell Assay |
Cell Viability Assay[4]
Cell Types: H35 and HepG2 cells Tested Concentrations: 1 μM Incubation Duration: 24 hrs (hours) Experimental Results: diminished mitochondrial cholesterol levels in H35 and HepG2 cells. |
| Animal Protocol |
Animal/Disease Models: SD (SD (Sprague-Dawley)) rat, body weight 150- 170 g[1]
Doses: 6.25, 12.5, 25 or 50 mg/kg Route of Administration: Single oral administration Experimental Results: In a dose-dependent manner in rats Acetate inhibits cholesterol biosynthesis. The ED50 value of YM-53601 for cholesterol biosynthesis inhibition is 32 mg/kg. Animal/Disease Models: Five to sixweeks old male BALB/c athymic (nu/nu) nude mice [4] Doses: 15 mg/kg Route of Administration: Daily po (oral gavage) treatment for 2 weeks Experimental Results: Dramatically diminished intratumoral Cholesterol levels. |
| References |
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| Molecular Formula |
C21H22CLFN2O
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|---|---|
| Molecular Weight |
372.86
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| Exact Mass |
372.14
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| CAS # |
182959-33-7
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| Related CAS # |
YM-53601 free base;182959-28-0
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| PubChem CID |
60196293
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| Appearance |
Off-white to light brown solid powder
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| LogP |
5.389
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
26
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| Complexity |
528
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1=CC=C2C(=C1)C3=C(C=C(C=C3)OC/C(=C/4\CN5CCC4CC5)/F)N2.Cl
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| InChi Key |
JWXYVHMBPISIJQ-TVWXOORISA-N
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| InChi Code |
InChI=1S/C21H21FN2O.ClH/c22-19(18-12-24-9-7-14(18)8-10-24)13-25-15-5-6-17-16-3-1-2-4-20(16)23-21(17)11-15;/h1-6,11,14,23H,7-10,12-13H2;1H/b19-18-;
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| Chemical Name |
2-[(2E)-2-(1-azabicyclo[2.2.2]octan-3-ylidene)-2-fluoroethoxy]-9H-carbazole;hydrochloride
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| Synonyms |
YM-53601 YM53601 YM 53601
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~268.20 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.6820 mL | 13.4099 mL | 26.8197 mL | |
| 5 mM | 0.5364 mL | 2.6820 mL | 5.3639 mL | |
| 10 mM | 0.2682 mL | 1.3410 mL | 2.6820 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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