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    XL413 (BMS-863233) HCl
    XL413 (BMS-863233) HCl

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    This product is for research use only, not for human use. We do not sell to patients.
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    InvivoChem Cat #: V1543
    CAS #: 1169562-71-3Purity ≥98%

    Description: XL413 HCl (BMS-863233) is a novel, highly potent and selective cell division cycle 7 homolog (CDC7) kinase inhibitor with IC50 of 3.4 nM, it shows 63-, 12- and 35-fold selectivity over CK2, Pim-1 and pMCM2, respectively. In MDA-MB-231T and Colo-205 cell lines, XL413 results in inhibition of CDC7 specific phosphorylation of MCM2. XL413 also inhibits the cell proliferation, decreases cell viability and elicits the caspase 3/7 activity in Colo-205 cells. Moreover, XL413 results in modified S phase progression that subsequently leads to apoptotic cell death.

    References: Bioorg Med Chem Lett. 2012 Jun 1;22(11):3727-31.

    Related CAS #: 1169562-71-3 (HCl)   1169558-38-6 (free base)   

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    Molecular Weight (MW)326.18 
    FormulaC14H13Cl2N3O2 
    CAS No.1169562-71-3 
    Storage-20℃ for 3 years in powder form
    -80℃ for 2 years in solvent
    Solubility (In vitro)DMSO: <1 mg/mL
    Water: 46 mg/mL (141.02 mM)
    Ethanol: <1 mg/mL
    Other infoChemical Name: (S)-8-chloro-2-(pyrrolidin-2-yl)benzofuro[3,2-d]pyrimidin-4(3H)-one hydrochloride

    InChi Key: UNDKJUKLBNARIZ-FVGYRXGTSA-N

    InChi Code: InChI=1S/C14H12ClN3O2.ClH/c15-7-3-4-10-8(6-7)11-12(20-10)14(19)18-13(17-11)9-2-1-5-16-9;/h3-4,6,9,16H,1-2,5H2,(H,17,18,19);1H/t9-;/m0./s1

    SMILES Code: O=C1C(OC2=CC=C(Cl)C=C23)=C3N=C([[email protected]]4NCCC4)N1.[H]Cl

    SynonymsXL413; XL-413; XL 413; BMS863233; BMS-863233; BMS 863233.


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    In Vitro

    In vitro activity: In MDA-MB-231T and Colo-205 cell lines, XL413 results in inhibition of CDC7 specific phosphorylation of MCM2. XL413 also inhibits the cell proliferation, decreases cell viability and elicits the caspase 3/7 activity in Colo-205 cells. Moreover, XL413 results in modified S phase progression that subsequently leads to apoptotic cell death.


    Kinase Assay: Kinase activity and compound inhibition are determined using the luciferase-luciferin-coupled chemiluminescence assay and measured as the percentage of ATP utilized following the kinase reaction in a 384-well format. The final CDC7 kinase assay condition is 6 nM CDC7/ASK, 1 μM ATP, 50 mM Hepes pH 7.4, 10 mM MgCl2, 0.02% BSA, 0.02% brij 35, 0.02% tween 20 and 1 mM DTT. It is worthy to note that the CDC7/ASK protein exhibits substrate-independent ATP utilization. All kinase reactions are incubated at room temperature for 1-2 h.


    Cell Assay: The cell proliferation is measured by BrdU incorporation assay, and viability is assayed by Cell Titer–Glo kits. Celllin used: Colo-205 cells.

    In VivoIn a Colo-205 xenograft model, XL413, at the 3 mg/kg dose, causes 70% inhibition of phosphorylated MCM2, and causes significant tumor growth regression at the 100 mg/kg dose.
    Animal modelColo-205 xenograft model
    Formulation & Dosage3, 100 mg/kg
    References

    Bioorg Med Chem Lett. 2012 Jun 1;22(11):3727-31. 


    These protocols are for reference only. InvivoChem does not independently validate these methods.

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