| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 25mg | |||
| Other Sizes |
Purity: ≥98%
| Targets |
Target: Vitamin D4 targets the vitamin D receptor (VDR) and functions as a VDR agonist. It regulates parathyroid hormone gene transcription in vivo. The compound is involved in calcium and phosphate homeostasis similar to other vitamin D forms. It is an active vitamin D analog.
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| ln Vitro |
Vitamin D inadequate and cardiovascular disease risk[2].
In Vitro Activity: Vitamin D4 demonstrates activity as a vitamin D analog. It binds to the vitamin D receptor and modulates gene expression. The compound is used to investigate differences in hepatic and target cell metabolism of vitamins D2 and D3. Detailed in vitro activity data are available from the primary literature. |
| ln Vivo |
Vitamin D metabolites control the rat's in vivo transcription of the parathyroid hormone gene[3].
In Vivo Activity: Vitamin D metabolites including Vitamin D4 control the rat's in vivo transcription of the parathyroid hormone gene. Vitamin D4 has been studied in vitamin D-resistant rickets. The compound is involved in calcium metabolism. Detailed in vivo efficacy data are available from the primary literature. |
| Enzyme Assay |
In Vitro Enzyme/Receptor Binding Protocol: Vitamin D receptor binding assays typically use recombinant VDR protein or cell extracts. Radioligand binding with [3H]1,25(OH)2D3 is performed with varying concentrations of Vitamin D4 to determine relative binding affinity. Metabolism studies compare hepatic and target cell metabolism of vitamins D2 and D3.
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| Cell Assay |
In Vitro Cell-Based Assay Protocol: Cells expressing VDR are treated with Vitamin D4 at various concentrations. VDR activation and downstream gene expression are measured using reporter gene assays or qPCR. Cell proliferation and differentiation assays may also be performed. Metabolism studies assess the compound's conversion and activity in different cell types.
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| Animal Protocol |
In Vivo Animal Assay Protocol: Animal models including vitamin D-resistant rickets models are used to study Vitamin D4. The compound is administered via appropriate routes, and calcium, phosphate, and parathyroid hormone levels are measured. Gene transcription of parathyroid hormone is assessed.
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| ADME/Pharmacokinetics |
Pharmacokinetics: Vitamin D4 has molecular formula C28H46O and molecular weight 398.66. It has density 0.96 g/cm3, boiling point 504.9°C, melting point 96-98°C, and logP 7.865. The precursor of Vitamin D4 is 22,23-dihydroergosterol. Detailed pharmacokinetic properties are similar to other vitamin D compounds.
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| Toxicity/Toxicokinetics |
Toxicity: No significant toxicity is associated with Vitamin D4 at physiological concentrations. As a naturally occurring vitamin D form, it is generally well-tolerated. High doses may cause hypercalcemia similar to other vitamin D compounds. The compound is for research use only.
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| References | |
| Additional Infomation |
Vitamin D4 is found in mushrooms. It is a fungal metabolite. It is a combination of vitamin D and an ergosterol compound.
Additional Information: Vitamin D4 has CAS number 511-28-4. It is also known as 22-Dihydroergocalciferol and VD4. The compound is present in mushrooms and functions as a fungal metabolite. It is produced by several mushroom species including A. bisporus from UV irradiation of 22,23-dihydroergosterol (Provitamin D4). Vitamin D4 is used in research to investigate differences in hepatic and target cell metabolism of vitamins D2 and D3. For research use only, not for human therapy. |
| Molecular Formula |
C28H46O
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|---|---|
| Molecular Weight |
398.66424
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| Exact Mass |
398.355
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| CAS # |
511-28-4
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| Related CAS # |
Vitamin D4-d5
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| PubChem CID |
5460703
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| Appearance |
Off-white to light yellow solid
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| Density |
0.96 g/cm3
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| Boiling Point |
504.9ºC at 760 mmHg
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| Melting Point |
96-98ºC
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| Flash Point |
219.2ºC
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| Index of Refraction |
1.52
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| LogP |
7.865
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
29
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| Complexity |
639
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| Defined Atom Stereocenter Count |
6
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| SMILES |
C=C1/C(C[C@@H](O)CC1)=C\C=C2[C@@]3([H])[C@@](CCC\2)(C)[C@@H]([C@H](C)CC[C@H](C)C(C)C)CC3
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| InChi Key |
DIPPFEXMRDPFBK-JPWDPSJFSA-N
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| InChi Code |
InChI=1S/C28H46O/c1-19(2)20(3)9-10-22(5)26-15-16-27-23(8-7-17-28(26,27)6)12-13-24-18-25(29)14-11-21(24)4/h12-13,19-20,22,25-27,29H,4,7-11,14-18H2,1-3,5-6H3/b23-12+,24-13-/t20-,22+,25-,26+,27-,28+/m0/s1
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| Chemical Name |
(1S,3Z)-3-[(2E)-2-[(1R,3aS,7aR)-1-[(2R,5S)-5,6-dimethylheptan-2-yl]-7a-methyl-2,3,3a,5,6,7-hexahydro-1H-inden-4-ylidene]ethylidene]-4-methylidenecyclohexan-1-ol
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| Synonyms |
Vitamin D4; 22-Dihydroergocalciferol; VD4
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~25 mg/mL (~62.7 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5084 mL | 12.5420 mL | 25.0840 mL | |
| 5 mM | 0.5017 mL | 2.5084 mL | 5.0168 mL | |
| 10 mM | 0.2508 mL | 1.2542 mL | 2.5084 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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