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500mg |
ln Vitro |
By triggering CRF2, urocortin II, mice induces positive inotropy and positive degeneration in ventricular muscle cells [1]. Mouse urocortin II activates CRF2 receptors in a manner that is reliant on both Ca2+/CaMKII and cAMP/PKA [1].
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ln Vivo |
The expression of Fos is sensed by Urocortin II, a mouse (1–10 μg; icv and iv; island SD electrode) [2]. Involves the central autonomic nerve system and Urocortin II, mouse (1 μg; icv; island electrode SD electrode)
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Animal Protocol |
Animal/Disease Models: Adult male SD (SD (Sprague-Dawley)) rat (250-300 g) [2]
Doses: 1, 5, 10 μg administered Method: intracerebroventricular injection and intravenous (iv) (iv)injection; abdominal control [2]. 1, 5 or 10 μg per animal dissolved in 2 μL of normal saline (for intravenous (iv) (iv)injection) or 200 μL (for intravenous (iv) (iv)injection). Experimental Results: Fos expression was induced in a dose-dependent manner. Animal/Disease Models: Adult male SD (SD (Sprague-Dawley)) rat (250-300 g) [2] Doses: 1 μg Route of Administration: Intracerebroventricular injection Experimental Results: diminished food intake over a 12-hour interval. |
References |
[1]. Yang LZ, et, al. cAMP- and Ca²(+) /calmodulin-dependent protein kinases mediate inotropic, lusitropic and arrhythmogenic effects of urocortin 2 in mouse ventricular myocytes. Br J Pharmacol. 2011 Jan;162(2):544-56.
[2]. Reyes TM, et, al. Urocortin II: a member of the corticotropin-releasing factor (CRF) neuropeptide family that is selectively bound by type 2 CRF receptors. Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2843-8. [3]. Reyes TM, et, al. Urocortin II: a member of the corticotropin-releasing factor (CRF) neuropeptide family that is selectively bound by type 2 CRF receptors. Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2843-8. |
Molecular Formula |
C187H320N56O50
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Molecular Weight |
4152.95
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CAS # |
330648-32-3
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 0.2408 mL | 1.2040 mL | 2.4079 mL | |
5 mM | 0.0482 mL | 0.2408 mL | 0.4816 mL | |
10 mM | 0.0241 mL | 0.1204 mL | 0.2408 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.