| Size | Price | Stock | Qty |
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| 10mg |
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| 25mg |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg | |||
| Other Sizes |
| Targets |
Uridine diphosphate glucuronic acid ammonium is a key metabolite and a central precursor in sugar nucleotide biosynthesis. It is a substrate for various enzymes, including C4-epimerases and decarboxylases. These enzymes convert UDP-GlcA to other sugar nucleotides, such as UDP-galacturonic acid (UDP-GalA) and UDP-pentose products, which are essential for the synthesis of polysaccharides. In pathogenic fungi like Cryptococcus neoformans, UDP-GlcA is a key metabolite for polysaccharide synthesis. Its role as a precursor for plant cell wall polysaccharides is also well-established.
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| ln Vitro |
In vitro, Uridine diphosphate glucuronic acid ammonium is used as a substrate in enzyme assays to study the activity of various enzymes involved in sugar nucleotide metabolism, such as C4-epimerases and decarboxylases. It is a key component in the synthesis of sugar nucleotides and polysaccharides. The compound is used in biochemical research to investigate the biosynthetic pathways of glycans and polysaccharides in both plants and fungi. It is also used as a standard in analytical chemistry for the detection and quantification of sugar nucleotides.
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| ln Vivo |
In vivo, Uridine diphosphate glucuronic acid ammonium is a naturally occurring metabolite involved in sugar nucleotide biosynthesis. It plays a critical role in the synthesis of polysaccharides in various organisms, including plants and fungi. In pathogenic fungi like Cryptococcus neoformans, it is a key metabolite for pod polysaccharide synthesis. Its in vivo role is essential for the production of cell wall components and other polysaccharides. However, it is not used as a therapeutic agent but as a research tool to study these metabolic pathways.
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| Enzyme Assay |
In vitro experiments with Uridine diphosphate glucuronic acid ammonium typically involve its use as a substrate in enzyme assays. For example, it is used to measure the activity of UDP-glucose dehydrogenase, the enzyme that produces it. In these assays, the enzyme is incubated with its substrate (UDP-glucose) and cofactor (NAD+), and the formation of UDP-GlcA is measured spectrophotometrically. UDP-GlcA is also used as a substrate for C4-epimerases and decarboxylases to study their activity. The compound is dissolved in an appropriate buffer, and the reaction products are analyzed by HPLC or mass spectrometry.
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| Cell Assay |
In vitro cell-based assays using Uridine diphosphate glucuronic acid ammonium are not typical, as the compound is primarily used as a substrate in enzyme assays rather than as a modulator of cellular function. However, it can be used to study the effects of manipulating sugar nucleotide levels on polysaccharide synthesis in cell culture. Cells (e.g., plant cells or fungal cells) could be treated with the compound or with inhibitors of its metabolism to assess the impact on cell wall or capsule formation. The compound is typically dissolved in an appropriate buffer for addition to cell culture media.
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| Animal Protocol |
Specific in vivo animal experiment protocols for Uridine diphosphate glucuronic acid ammonium are not detailed in the available literature. The compound is a naturally occurring metabolite and is not typically administered as a drug. Its study in vivo would involve investigating its role in metabolic pathways, such as by using isotopic labeling to track its incorporation into polysaccharides. For example, labeled UDP-GlcA could be administered to animals or plants to study polysaccharide biosynthesis. The route of administration and dosing would depend on the specific experimental design.
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| ADME/Pharmacokinetics |
Uridine diphosphate glucuronic acid ammonium has the molecular formula C15H22N2O18P2·xNH3 and is also known as UDP-α-D-glucuronic acid ammonium. It is a central precursor in sugar nucleotide biosynthesis and is a cofactor formed by UDP-glucose dehydrogenase. The compound is soluble in water and is typically stored at -20°C. Its pharmacokinetic properties have not been extensively characterized, as it is a naturally occurring metabolite rather than a drug.
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| Toxicity/Toxicokinetics |
Specific toxicological data for Uridine diphosphate glucuronic acid ammonium are not provided in the available sources. As a naturally occurring metabolite, it is likely to have a low toxicity profile. However, as a research chemical, it is intended for laboratory use only and is not for human consumption. Standard laboratory safety precautions should be followed when handling this compound, including the use of appropriate personal protective equipment. Its purity is typically >98%.
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| References | |
| Additional Infomation |
Uridine diphosphate glucuronic acid ammonium (UDP-GlcA ammonium) is a central precursor in sugar nucleotide biosynthesis. It has the molecular formula C15H22N2O18P2·xNH3 and is also known as UDP-α-D-glucuronic acid ammonium. UDP-GlcA is a cofactor formed by the catalytic activity of UDP-glucose dehydrogenase. It serves as a common substrate for C4-epimerases and decarboxylases, which release UDP-galacturonic acid (UDP-GalA) and UDP-pentose products, respectively. It is a key metabolite for pod polysaccharide synthesis in the pathogenic fungus Cryptococcus neoformans and is a precursor of many plant cell wall polysaccharides. The compound is used in research to study sugar nucleotide metabolism and polysaccharide biosynthesis and is intended for research use only.
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| CAS # |
43195-60-4
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| Related CAS # |
Uridine diphosphate galuronic acid trisodium;148407-07-2
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| PubChem CID |
71312081
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| Appearance |
Typically exists as solid at room temperature
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| LogP |
0
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| Hydrogen Bond Donor Count |
10
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| Hydrogen Bond Acceptor Count |
19
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| Rotatable Bond Count |
9
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| Heavy Atom Count |
38
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| Complexity |
1040
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| Defined Atom Stereocenter Count |
9
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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