| Size | Price | Stock | Qty |
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| 100mg |
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| 250mg | |||
| 500mg | |||
| 1g | |||
| Other Sizes |
| Targets |
Uniblue A sodium targets proteins for staining applications. As a reactive protein dye, it covalently binds to proteins, allowing for their detection and visualization in gels. The compound has also been found to possess various biological activities, including anti-inflammatory, anti-tumor, and anti-microbial properties. Its mechanism of action for these activities is not fully detailed. The dye may also interact with the P2X purinoceptor 1 (P2RX1).
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| ln Vitro |
Advice (This is our suggested protocol, which should be adjusted to suit your particular requirements as it simply offers guidance) [1]. 1. Make the derivatization buffer (pH 8–9), 10% SDS, and 100 mM NaHCO3. 2. To 90 μL of protein solution, add 10 μL of dissolved 200 mM Uniblue A solution in derivatization buffer. 3. To stain, heat the sample for one minute at 100°C. 4. Pour in 100 μL of a reducing solution (20 mM DTT and 10% glycerol) in 200 mM Tris buffer (pH 6.8). 5. In electrophoresis, excess Uniblue A combines with Tris to form a blue compound that serves as an electrophoresis front indicator. 6. For effective reduction, heat the sample for an extra minute at 100°C, then let it cool to room temperature. 7. Incubate for five minutes after adding 20 μL of an alkylation solution containing 550 mM iodoacetamide (IAA). 8. Apply SDS-PAGE to the sample. Note: Derivatization buffer can be used to directly dilute protein samples that are dried or in compatible buffers (i.e., free of amines) to a concentration of 5 mg/mL. In other situations, it is advised to perform a prior acetone/trichloroacetic acid (TCA) precipitation or buffer exchange via ultrafiltration (see below). Bovine serum albumin (BSA) in derivatization buffer at a concentration of 10 mg/mL was utilized as a positive control.
In vitro, Uniblue A sodium is used as a reactive protein stain for covalent pre-gel staining of proteins. The dye exhibits excitation at 594 nm, allowing for fluorescence-based detection. The compound is used in biochemical research for protein staining applications. Uniblue A sodium has also been found to possess anti-inflammatory, anti-tumor, and anti-microbial properties in various biological assays. |
| ln Vivo |
Uniblue A sodium is not used as a therapeutic agent and has no established in vivo pharmacological activity. Its primary application is as a research tool for protein staining in biochemical research. The compound has been found to possess various biological activities, including anti-inflammatory, anti-tumor, and anti-microbial properties. It is not administered to animals for therapeutic purposes but is used in the preparation of solutions for biological experiments.
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| Enzyme Assay |
In vitro experiments with Uniblue A sodium typically involve its use as a protein stain in gel electrophoresis. Proteins are separated by SDS-PAGE, and the gel is stained with Uniblue A sodium for covalent pre-gel staining. The dye covalently binds to proteins, allowing for their detection. The stained proteins can be visualized by fluorescence with excitation at 594 nm. The compound is typically dissolved in appropriate buffers for staining.
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| Cell Assay |
Uniblue A sodium is not typically used in cell-based assays as it is primarily a protein stain for gel electrophoresis. However, its biological activities, including anti-inflammatory, anti-tumor, and anti-microbial properties, could be studied in cell-based assays. The compound could be tested for its effects on cell viability, inflammation, and microbial growth. The compound is typically dissolved in DMSO or water for preparation of stock solutions.
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| Animal Protocol |
Specific in vivo animal experiment protocols for Uniblue A sodium are not detailed in the available literature. The compound is primarily used as a protein stain for in vitro applications. Its potential biological activities could be studied in animal models, but such applications are not standard.
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| ADME/Pharmacokinetics |
Uniblue A sodium has a molecular weight of 506.48 g/mol and the formula C22H15N2NaO7S2. The compound is also known as Acid Blue 215. It exhibits excitation at 594 nm. For storage, the compound is kept at room temperature. Its purity is typically ≥70%. Uniblue A sodium is a reactive protein dye used for covalent pre-gel staining of proteins. It has been found to possess various biological activities, including anti-inflammatory, anti-tumor, and anti-microbial properties.
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| Toxicity/Toxicokinetics |
Specific toxicological data for Uniblue A sodium are not provided in the available sources. As a research chemical, it is intended for laboratory use only and is not for human consumption. Standard laboratory safety precautions should be followed when handling this compound, including the use of gloves and eye protection. The dye may cause irritation to skin, eyes, and respiratory tract upon contact.
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| References | |
| Additional Infomation |
Uniblue A sodium (CAS 14541-90-3) is a reactive protein dye that can be used for covalent pre-gel staining of proteins. It has the molecular formula C22H15N2NaO7S2 and a molecular weight of 506.48 g/mol. The dye exhibits excitation at 594 nm. Uniblue A sodium has been found to possess various biological activities, including anti-inflammatory, anti-tumor, and anti-microbial properties. The compound is also known as Acid Blue 215. It is used in biochemical research for protein staining applications. The compound is intended for research use only.
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| Molecular Formula |
C22H16N2O7S2.NA
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|---|---|
| Molecular Weight |
506.48300
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| Exact Mass |
506.022
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| CAS # |
14541-90-3
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| PubChem CID |
23672572
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| Appearance |
Typically exists as solid at room temperature
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| Melting Point |
>300ºC(lit.)
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| LogP |
5.424
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
34
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| Complexity |
1020
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C=CS(C1C=CC=C(NC2=CC(S([O-])(=O)=O)=C(N)C3C(C4=CC=CC=C4C(=O)C2=3)=O)C=1)(=O)=O
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| InChi Key |
ANOULJGZTVOIFB-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C22H16N2O7S2.Na/c1-2-32(27,28)13-7-5-6-12(10-13)24-16-11-17(33(29,30)31)20(23)19-18(16)21(25)14-8-3-4-9-15(14)22(19)26;/h2-11,24H,1,23H2,(H,29,30,31);/q;+1/p-1
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| Chemical Name |
sodium;1-amino-4-(3-ethenylsulfonylanilino)-9,10-dioxoanthracene-2-sulfonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.9744 mL | 9.8721 mL | 19.7441 mL | |
| 5 mM | 0.3949 mL | 1.9744 mL | 3.9488 mL | |
| 10 mM | 0.1974 mL | 0.9872 mL | 1.9744 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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