| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| 1g |
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| Other Sizes |
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| ln Vitro |
Advice (This is our suggested protocol, which should be adjusted to suit your particular requirements as it simply offers guidance) [1]. 1. Make the derivatization buffer (pH 8–9), 10% SDS, and 100 mM NaHCO3. 2. To 90 μL of protein solution, add 10 μL of dissolved 200 mM Uniblue A solution in derivatization buffer. 3. To stain, heat the sample for one minute at 100°C. 4. Pour in 100 μL of a reducing solution (20 mM DTT and 10% glycerol) in 200 mM Tris buffer (pH 6.8). 5. In electrophoresis, excess Uniblue A combines with Tris to form a blue compound that serves as an electrophoresis front indicator. 6. For effective reduction, heat the sample for an extra minute at 100°C, then let it cool to room temperature. 7. Incubate for five minutes after adding 20 μL of an alkylation solution containing 550 mM iodoacetamide (IAA). 8. Apply SDS-PAGE to the sample. Note: Derivatization buffer can be used to directly dilute protein samples that are dried or in compatible buffers (i.e., free of amines) to a concentration of 5 mg/mL. In other situations, it is advised to perform a prior acetone/trichloroacetic acid (TCA) precipitation or buffer exchange via ultrafiltration (see below). Bovine serum albumin (BSA) in derivatization buffer at a concentration of 10 mg/mL was utilized as a positive control.
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|---|---|
| References |
| Molecular Formula |
C22H16N2O7S2.NA
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|---|---|
| Molecular Weight |
506.48300
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| Exact Mass |
506.022
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| CAS # |
14541-90-3
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| PubChem CID |
23672572
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| Appearance |
Typically exists as solid at room temperature
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| Melting Point |
>300ºC(lit.)
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| LogP |
5.424
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
34
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| Complexity |
1020
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C=CS(C1C=CC=C(NC2=CC(S([O-])(=O)=O)=C(N)C3C(C4=CC=CC=C4C(=O)C2=3)=O)C=1)(=O)=O
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| InChi Key |
ANOULJGZTVOIFB-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C22H16N2O7S2.Na/c1-2-32(27,28)13-7-5-6-12(10-13)24-16-11-17(33(29,30)31)20(23)19-18(16)21(25)14-8-3-4-9-15(14)22(19)26;/h2-11,24H,1,23H2,(H,29,30,31);/q;+1/p-1
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| Chemical Name |
sodium;1-amino-4-(3-ethenylsulfonylanilino)-9,10-dioxoanthracene-2-sulfonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.9744 mL | 9.8721 mL | 19.7441 mL | |
| 5 mM | 0.3949 mL | 1.9744 mL | 3.9488 mL | |
| 10 mM | 0.1974 mL | 0.9872 mL | 1.9744 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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