| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| 250mg | |||
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| Targets |
TSQ does not have a specific biological target. It is a fluorescent probe that selectively binds to zinc ions (Zn²⁺). It accumulates in cells in a zinc content-dependent manner. By binding to Zn²⁺, it produces a fluorescent signal that can be used to visualize and quantify intracellular zinc levels. It is used in research to study zinc biology.
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| ln Vitro |
For TSQ, a working concentration of 0.1 μM–10 μM is advised.
In vitro, TSQ is used as a fluorescent probe to detect and image intracellular zinc ions. It is membrane-permeable and can be used to measure extracellular and intracellular Zn²⁺ levels in biological systems. It produces blue fluorescence upon binding to Zn²⁺. Specific activity data are not applicable. |
| ln Vivo |
In vivo applications of TSQ are not typical, as it is primarily used in vitro for imaging zinc in cells or tissues. It is not administered to living animals for therapeutic or diagnostic purposes. Its use is confined to laboratory research settings.
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| Enzyme Assay |
A cell-free assay for TSQ would involve measuring its fluorescence upon binding to Zn²⁺. In such an assay, the probe is incubated with varying concentrations of Zn²⁺, and the increase in fluorescence is measured. This can be used to quantify zinc levels in samples. Its selectivity for Zn²⁺ over other metal ions can also be assessed.
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| Cell Assay |
Cellular experiments with TSQ typically involve loading cells with the probe and imaging them using fluorescence microscopy. Cells are incubated with TSQ, which enters the cells and binds to intracellular Zn²⁺. The resulting blue fluorescence can be visualized and quantified. The probe is used to study zinc homeostasis and signaling in various cell types.
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| Animal Protocol |
In vivo animal experiments for TSQ are not applicable, as it is a fluorescent probe used in vitro on cells or tissue sections.
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| ADME/Pharmacokinetics |
TSQ is a small molecule with a molecular weight of 328.39. Its pharmacokinetic properties are not relevant as it is not a therapeutic drug. The compound is used in vitro for research applications. It should be stored at 0-10°C.
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| Toxicity/Toxicokinetics |
Toxicity data for TSQ are not provided in the available sources. As a fluorescent probe, it is generally considered to have low toxicity for its intended research applications. However, specific toxicological information is not available. The compound is intended for research use only and not for human consumption.
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| References | |
| Additional Infomation |
TSQ (CAS: 109628-27-5) is a cytosolic zinc ion fluorescence probe. It is also known as N-(6-Methoxy-8-quinolyl)-4-toluenesulfonamide. The probe is membrane-permeable and can be used for intracellular imaging of zinc proteins. TSQ selectively binds to Zn²⁺ in the presence of Ca²⁺ and Mg²⁺, producing blue fluorescence. It has an excitation/emission maximum of approximately 334/495 nm. Its molecular formula is C17H16N2O3S.
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| Molecular Formula |
C17H16N2O3S
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|---|---|
| Molecular Weight |
328.38554
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| Exact Mass |
328.088
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| CAS # |
109628-27-5
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| PubChem CID |
130606
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| Appearance |
Light brown to brown solid powder
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| Density |
1.334g/cm3
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| Boiling Point |
518.8ºC at 760mmHg
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| Melting Point |
133–134 °C (lit.)
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| Flash Point |
267.5 °C - closed cup (lit.)
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| Vapour Pressure |
7.27E-11mmHg at 25°C
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| Index of Refraction |
1.654
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| LogP |
4.506
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
23
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| Complexity |
483
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
HKRNYOZJJMFDBV-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C17H16N2O3S/c1-12-5-7-15(8-6-12)23(20,21)19-16-11-14(22-2)10-13-4-3-9-18-17(13)16/h3-11,19H,1-2H3
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| Chemical Name |
N-(6-methoxyquinolin-8-yl)-4-methylbenzenesulfonamide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 100 mg/mL (~304.52 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0452 mL | 15.2258 mL | 30.4516 mL | |
| 5 mM | 0.6090 mL | 3.0452 mL | 6.0903 mL | |
| 10 mM | 0.3045 mL | 1.5226 mL | 3.0452 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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