| Size | Price | Stock | Qty |
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| 5mg |
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| Other Sizes |
| Targets |
Target: His-tagged proteins (polyhistidine tag, typically 6xHis or 10xHis). Tris-NTA contains three NTA groups that coordinate Ni2+ ions. When Ni2+ is loaded, Tris-NTA forms a trivalent chelating platform that interacts with the His-tag on recombinant proteins with dramatically increased avidity (picomolar KD) compared to monovalent NTA (micromolar KD). This enables purification, immobilization, and detection of His-tagged proteins.
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| ln Vitro |
His-tagged proteins can be bound by the His-tagged protein ligand Tris-NTA [1][2].
In vitro, Tris-NTA is a His-tagged protein ligand used to bind and capture His-tagged proteins from cell lysates or conditioned media. The compound can be used in affinity chromatography, protein pull-down assays, surface plasmon resonance (SPR) biosensors, and protein labeling. It dramatically increases the affinity and selectivity for His-tagged proteins (KD in the picomolar range) compared to monovalent Ni-NTA (KD ∼10-20 uM). No direct pharmacological activity is reported. |
| ln Vivo |
No in vivo studies have been performed with Tris-NTA. This compound is not intended for in vivo administration; it is a biochemical reagent for protein purification and analysis in vitro. It is used exclusively in laboratory research for protein biochemistry and molecular biology applications. No animal studies have been reported.
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| Enzyme Assay |
For cell-free assays (protein purification): Tris-NTA resin or beads are equilibrated with lysis buffer (50 mM Tris-HCl, pH 8.0, 300 mM NaCl, 10 mM imidazole, 0.1% Triton X-100). Cell lysates containing His-tagged protein are added to the resin and incubated for 30-60 min at 4degC with rotation. Beads are washed with wash buffer (20-40 mM imidazole) to remove non-specifically bound proteins. Bound protein is eluted with elution buffer (250-500 mM imidazole, pH 8.0). Purified fractions are analyzed by SDS-PAGE and Western blot. For SPR immobilization: Tris-NTA is immobilized on a gold sensor chip via thiol groups, loaded with Ni2+, and used to capture His-tagged proteins for kinetic analysis.
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| Cell Assay |
No cell-based assays are performed with Tris-NTA. This reagent is used for protein purification from cell lysates, not for direct addition to living cells. His-tagged proteins purified using Tris-NTA may be subsequently added to cells for functional studies, but the Tris-NTA reagent itself is not used in cell-based assays.
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| Animal Protocol |
No animal studies are conducted with Tris-NTA. The compound is used exclusively in vitro for protein biochemistry research. It is not administered to animals for efficacy or safety studies. Its use is confined to laboratory bench applications.
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| ADME/Pharmacokinetics |
PK properties are not applicable for Tris-NTA, as it is not a drug candidate and is not intended for systemic administration. It is a research reagent used in vitro. No absorption, distribution, metabolism, or excretion studies have been performed. The compound is for research use only.
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| Toxicity/Toxicokinetics |
No toxicity data are available for Tris-NTA. As a biochemical reagent used in protein purification, it is not intended for human consumption. The compound contains NTA groups, which can chelate metal ions and may have mild toxicity if ingested. However, no formal toxicity studies have been performed. The compound is for research use only and should be handled with appropriate laboratory safety precautions.
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| References |
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| Additional Infomation |
Tris-NTA is a research reagent, not a drug. It is not FDA-approved for clinical use. It is widely used in molecular biology, protein biochemistry, and biophysics for purification, immobilization, and analysis of His-tagged recombinant proteins. Applications include affinity chromatography, protein interaction studies (SPR, BLI), protein labeling (fluorescent tags), and biosensor development. The high-affinity Tris-NTA platform enables robust and sensitive detection of His-tagged proteins even in complex biological samples.
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| Molecular Formula |
C43H68N8O22
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|---|---|
| Molecular Weight |
1049.04043292999
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| Exact Mass |
1048.44
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| CAS # |
862778-60-7
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| Related CAS # |
(Rac)-Tris-NTA;1146592-99-5
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| PubChem CID |
124202503
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| Appearance |
White to light yellow solid powder
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| LogP |
-12.9
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| Hydrogen Bond Donor Count |
10
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| Hydrogen Bond Acceptor Count |
26
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| Rotatable Bond Count |
32
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| Heavy Atom Count |
73
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| Complexity |
1910
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| Defined Atom Stereocenter Count |
3
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| SMILES |
C1CN(CCN(CCCN(CCN(C1)C(=O)CC[C@@H](C(=O)O)N(CC(=O)O)CC(=O)O)C(=O)CC[C@@H](C(=O)O)N(CC(=O)O)CC(=O)O)C(=O)CC[C@@H](C(=O)O)N(CC(=O)O)CC(=O)O)C(=O)CCCCCN
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| InChi Key |
SRHQOOQNBLZULK-DTXPUJKBSA-N
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| InChi Code |
InChI=1S/C43H68N8O22/c44-13-3-1-2-6-31(52)45-14-4-15-47(33(54)11-8-29(42(70)71)50(24-37(60)61)25-38(62)63)20-21-48(34(55)12-9-30(43(72)73)51(26-39(64)65)27-40(66)67)17-5-16-46(19-18-45)32(53)10-7-28(41(68)69)49(22-35(56)57)23-36(58)59/h28-30H,1-27,44H2,(H,56,57)(H,58,59)(H,60,61)(H,62,63)(H,64,65)(H,66,67)(H,68,69)(H,70,71)(H,72,73)/t28-,29-,30-/m0/s1
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| Chemical Name |
(2S)-5-[11-(6-aminohexanoyl)-4,8-bis[(4S)-4-[bis(carboxymethyl)amino]-4-carboxybutanoyl]-1,4,8,11-tetrazacyclotetradec-1-yl]-2-[bis(carboxymethyl)amino]-5-oxopentanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~47.66 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 0.9533 mL | 4.7663 mL | 9.5325 mL | |
| 5 mM | 0.1907 mL | 0.9533 mL | 1.9065 mL | |
| 10 mM | 0.0953 mL | 0.4766 mL | 0.9533 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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