| Size | Price | Stock | Qty |
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| 5g |
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| Other Sizes |
| Targets |
TOPS is a chromogenic substrate that reacts with 4-aminoantipyrine in the presence of H2O2 and peroxidase to produce a colored product. The compound binds to cholesterol esters and inhibits their hydrolysis by cholesterol esterase. Its active form inhibits prostaglandin E2 production in THP-1 and Caco-2 cells, possibly due to its peroxidase-like activity. TOPS is used as a reagent in diagnostic tests for hydrogen peroxide, uric acid, and cholesterol. It is a Trinder's reagent widely used in clinical chemistry.
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| ln Vitro |
In vitro, TOPS undergoes an oxidative coupling reaction with 4-aminoantipyrine in the presence of H2O2 and peroxidase. The compound binds to cholesterol esters, inhibiting their hydrolysis by cholesterol esterase. Its active form inhibits prostaglandin E2 production in THP-1 and Caco-2 cells. TOPS is used as a chromogenic substrate in diagnostic tests and biochemical assays. Its peroxidase-like activity contributes to its bioactivity. The compound is highly water-soluble.
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| ln Vivo |
In vivo, TOPS is used as a diagnostic reagent in clinical chemistry tests. It is widely used in diagnostic tests and biochemical tests. The compound’s ability to detect hydrogen peroxide, uric acid, and cholesterol makes it valuable for clinical diagnostics. Its high water solubility and stability enhance its utility in various applications. TOPS is not typically used as a therapeutic agent but as a diagnostic reagent.
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| Enzyme Assay |
TOPS’s in vitro assays involve oxidative coupling reactions with 4-aminoantipyrine in the presence of H2O2 and peroxidase. The reaction produces a colored product that is measured spectrophotometrically. The compound’s efficacy as a Trinder's reagent is assessed by measuring the absorbance at specific wavelengths. For cholesterol esterase inhibition, enzyme activity is measured by monitoring cholesterol ester hydrolysis. For prostaglandin E2 inhibition, ELISA-based assays are performed in cell culture supernatants. Assays are performed in appropriate buffer systems with positive controls.
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| Cell Assay |
TOPS’s in vitro cell-based assays are conducted in THP-1 monocytes and Caco-2 intestinal cells. Cells are cultured in appropriate media and treated with TOPS at varying concentrations. Prostaglandin E2 levels in culture supernatants are measured by ELISA. Cell viability is assessed by standard assays. The compound’s peroxidase-like activity is evaluated. Experiments are performed in triplicate with appropriate controls. The compound’s effects on cholesterol metabolism may also be assessed in appropriate cell models.
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| Animal Protocol |
TOPS is used as a diagnostic reagent in clinical settings. Its utility in detecting hydrogen peroxide, uric acid, and cholesterol has been validated in various diagnostic tests. The compound is widely used in biochemical tests and clinical diagnostics. Its high water solubility and stability make it suitable for use in aqueous assay systems. TOPS is not used as a therapeutic agent but as a research and diagnostic reagent.
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| ADME/Pharmacokinetics |
TOPS (MW 279.33 g/mol, C12H18NNaO3S) is a highly water-soluble aniline derivative. The compound is a Trinder's reagent widely used in diagnostic tests. It is stable under recommended storage conditions. TOPS is typically used as a solution in aqueous buffers. The compound’s high water solubility enhances its utility in colorimetric assays. Pharmacokinetic properties are not typically characterized as it is a diagnostic reagent.
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| Toxicity/Toxicokinetics |
TOPS is generally considered safe as a laboratory reagent. The compound is widely used in diagnostic tests and biochemical assays. No significant adverse effects have been reported at research-use concentrations. The compound is intended for research use only. Standard laboratory safety practices should be employed when handling this compound. Comprehensive toxicological evaluation would be required for therapeutic development.
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| Additional Infomation |
TOPS is a Trinder's reagent, a highly water-soluble aniline derivative widely used in diagnostic tests and biochemical tests. It is used for the colorimetric determination of hydrogen peroxide, uric acid, and cholesterol. TOPS undergoes an oxidative coupling reaction with 4-aminoantipyrine in the presence of H2O2 and peroxidase. The compound binds to cholesterol esters and inhibits their hydrolysis. Its active form inhibits prostaglandin E2 production in THP-1 and Caco-2 cells. All applications are limited to non-human research use.
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| Molecular Formula |
C12H18NNAO3S
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|---|---|
| Molecular Weight |
279.3310
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| Exact Mass |
279.09
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| CAS # |
40567-80-4
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| PubChem CID |
23679008
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| Appearance |
White to off-white solid powder
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| LogP |
2.837
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
18
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| Complexity |
316
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CCN(CCCS(=O)(=O)[O-])C1=CC=CC(=C1)C.[Na+]
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| InChi Key |
NJZLCEFCAHNYIR-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C12H19NO3S.Na/c1-3-13(8-5-9-17(14,15)16)12-7-4-6-11(2)10-12;/h4,6-7,10H,3,5,8-9H2,1-2H3,(H,14,15,16);/q;+1/p-1
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| Chemical Name |
sodium;3-(N-ethyl-3-methylanilino)propane-1-sulfonate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 42 mg/mL (~150.36 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.5800 mL | 17.9000 mL | 35.7999 mL | |
| 5 mM | 0.7160 mL | 3.5800 mL | 7.1600 mL | |
| 10 mM | 0.3580 mL | 1.7900 mL | 3.5800 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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